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A method for high-yield cellulase utilizing Trichoderma fungus

A technology of cellulase and Trichoderma, which is applied in the field of high-yield cellulase using Trichoderma fungus, can solve problems affecting the popularization and application of cellulase, increase the cost of cellulase, increase production costs, etc., and achieve improved induction effect , Enzyme system structure is perfect, the effect of saving production cost

Active Publication Date: 2018-01-05
湖南省核农学与航天育种研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Cellulase is an inducing compound enzyme. Different substrates have different inducing effects on different enzymes in the enzyme system. At present, corn stalks, wheat straws and other crop straws are most used for induction, but the effect is not ideal, so many Companies are increasingly using wood as an alternative inducer
On the one hand, this will increase production costs, leading to an increase in the cost of cellulase, which will directly affect the popularization and application of cellulase. On the one hand, it will lead to a shortage of forest resources, which is not conducive to environmental protection.

Method used

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  • A method for high-yield cellulase utilizing Trichoderma fungus

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Experimental program
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Effect test

Embodiment 1

[0045] a kind of like figure 1 Shown utilizes the method for Trichoderma viride (Trichoderma viride) high-yield cellulase, comprises the following steps:

[0046] (1) The spores of Trichoderma viride were inoculated on the PDA plate culture medium in a sterile environment, cultured with shaking at 30° C. for 96 hours, and then a ring was picked and inoculated in the seed medium. The production process of the seed medium is as follows: cut 200g of potatoes into small pieces, add 800ml of water, boil for 30min, filter with gauze, add 20g of glucose after the filtrate is cooled, dilute to 1L, and sterilize at 120°C for 15min. The shaker culture was carried out in the seed medium, and the temperature was 30° C., and the rotation speed was 130 r / min. The shaker was cultured for 48 hours, and the seed liquid was obtained after the shaker culture.

[0047](2) Insert the seed liquid obtained in step (1) into the fermented enzyme production medium according to the amount of 5% of the ...

Embodiment 2

[0058] a kind of like figure 1 Shown utilize Trichoderma reesei (Trichoderma reesei) high-yield cellulase method, comprises the following steps:

[0059] (1) The spores of Trichoderma reesei were inoculated on a PDA plate culture medium under a sterile environment, cultured with shaking at 28° C. for 96 hours, and then a loop was picked and inoculated in the seed medium. The production process of the seed medium is as follows: cut 200g of potatoes into small pieces, add 800ml of water, boil for 30min, filter with gauze, add 20g of glucose after the filtrate is cooled, dilute to 1L, and sterilize at 120°C for 15min. The shaker culture was carried out in the seed medium, and the temperature was 28° C., and the rotation speed was 130 r / min. The shaker was cultured for 48 hours, and the seed liquid was obtained after the shaker culture.

[0060] (2) Insert the seed solution obtained in step (1) into the fermented enzyme production medium according to the insertion amount of 4%, a...

Embodiment 3

[0071] a kind of like figure 1 Shown utilizes Trichoderma koningi (Trichoderma koningi) high-yield cellulase method, comprises the following steps:

[0072] (1) The spores of Trichoderma konii were inoculated on a PDA plate medium for culture in a sterile environment, cultured with shaking at 30° C. for 96 hours, and then a loop was picked and inoculated in the seed medium. The production process of the seed medium is as follows: cut 200g of potatoes into small pieces, add 800ml of water, boil for 30min, filter with gauze, add 20g of glucose after the filtrate is cooled, dilute to 1L, and sterilize at 120°C for 15min. The shaker culture was carried out in the seed medium, and the temperature was 30° C., and the rotation speed was 130 r / min. The shaker was cultured for 48 hours, and the seed liquid was obtained after the shaker culture.

[0073] (2) Insert the seed solution obtained in step (1) into the fermented enzyme production medium according to the amount of 6% of the in...

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Abstract

The invention discloses a method for high-yielding cellulose through trichoderma fungi. The irradiation technology is introduced to modify rice straw, the inducing effect is improved, and the segmentation inducing fermentation technology is adopted. The method specifically comprises the following steps that under the aseptic environment, spores of the trichoderma fungi are inoculated to a PDA plating medium to be cultured, a circle of spores are picked to be inoculated to a seed medium, and seed liquid is obtained after shaking table shake culturing is conducted; the obtained seed liquid is inoculated to a fermentation enzyme producing culture medium containing lactose to be subjected to fermentation cultivation according to a certain inoculation amount; a fresh carbon source containing sophorose is added to a fermentation system through aseptic operation, and fermentation cultivation continues to be conducted; after fermentation is finished, the obtained fermentation liquid is subjected to centrifugal separation, and obtained supernate is the high-yielding cellulose enzyme liquid. A cellulose enzyme system obtained through the method is more perfect in structure and high in yield.

Description

technical field [0001] The invention relates to the technical field of cellulase fermentation production, in particular to a method for high-yielding cellulase by using Trichoderma fungus. Background technique [0002] Cellulase is the key catalyst to catalyze the conversion of cellulose in straw into sugar and realize the energy conversion of straw, mainly produced by microorganisms. At present, cellulase in China is mainly used in textile, feed, food and other fields, and the annual output is low, which is difficult to meet the current market demand. If the key technology of straw energy conversion is broken through, the market demand for cellulase will rise in a straight line, and it is predicted that the demand will reach 250,000 to 400,000 tons per year, and the market prospect is huge. [0003] Cellulase is an inducing compound enzyme. Different substrates have different inducing effects on different enzymes in the enzyme system. At present, corn stalks, wheat straws ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/42C12R1/885
CPCC12N9/2437
Inventor 陈亮王克勤武小芬张勇苏小军胡秋龙李文革
Owner 湖南省核农学与航天育种研究所
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