Preserving fluid and preparation method therefor

A technology for preserving liquid and solution, applied in the field of stem cell preparation, can solve the problems of separation operation interference, affecting cell activity, and lack of nutrients, avoiding shape variation or differentiation, fast preparation process, and preventing blood agglutination.

Inactive Publication Date: 2015-11-11
SHEN ZHEN ISTEM REGENERATIVE MEDICINE SCI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, normal saline can maintain an osmotic pressure environment in a short period of time, but it does not have nutrients and cannot provide the nutritional needs of tissues or cells; while culture medium is rich in nutrients and can provide cell metabolism, but acidic products accumulate during tissue metabolism. It is easy to cause the pH to drop, thereby affecting cell viability
These two types of preservation solutions are insufficient in maintaining the osmotic pressure of the extracellular environment and resisting pollution. In particular, they cannot inhibit the blood coagulation of the placenta and umbilical cord tissue, which often makes the blood coagulation of the placenta and umbilical cord tissue interfere with the subsequent separation operation.

Method used

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  • Preserving fluid and preparation method therefor

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preparation example Construction

[0025] On the basis of the above-mentioned preservation solution components, the present invention further proposes a preparation method of the above-mentioned preservation solution, including the following steps (configured according to a standard volume of 1 L):

[0026] S01, according to the amount described in the above preservation solution, weigh 6-7g sodium chloride, 200-250mg calcium chloride, 200-400mg potassium chloride, 1-1.2g glucose one by one, and then make the first solution with water;

[0027] S02, according to the amount described in the above storage solution, weigh 10-30 g of human serum albumin, dissolve it in water to make a second solution;

[0028] S03, taking 50-60g of dextran powder and dissolving it in water to make a third solution;

[0029] S04, make antibiotic solution with a small amount of water according to the solid antibiotic in the above preservation solution;

[0030] S05, after mixing the first solution, the second solution, the third solut...

Embodiment 1

[0036] S01, take penicillin for injection (800,000 units / bottle), add 4 mL of water for injection, fully dissolve to a concentration of 200,000 units / mL, store at -20°C for later use;

[0037] Take streptomycin sulfate for injection (1g / bottle), add 5mL of water for injection, fully dissolve to a concentration of 200mg / mL, store at -20°C for later use.

[0038] S02, take 0.15L water for injection, weigh one by one and add 6g sodium chloride, 200mg calcium chloride, 400mg potassium chloride, 1g glucose, stir with a magnetic stirrer until the solution is uniform and transparent to obtain solution A;

[0039] S03, take 0.2L of water for injection, weigh and add 30g of human serum albumin, stir with a magnetic stirrer until the solution is uniform and transparent to obtain solution B;

[0040] S04, take 0.5L of water for injection, weigh and add 50g of dextran powder, stir with a magnetic stirrer until the solution is uniform and transparent to obtain solution C.

[0041] S05, tr...

Embodiment 2

[0044] S01, take 2.5 mL of purchased liquid gentamicin and pre-make 10 mL of gentamicin solution.

[0045] S02, take 0.15L water for injection, weigh one by one and add 7g sodium chloride, 250mg calcium chloride, 200mg potassium chloride, 1.2g glucose, stir with a magnetic stirrer until the solution is uniform and transparent to obtain solution A;

[0046] S03, take 0.2L of water for injection, weigh and add 10g of human serum albumin, stir with a magnetic stirrer until the solution is uniform and transparent to obtain solution B;

[0047] S04, take 0.5L of water for injection, weigh and add 60g of dextran powder, stir with a magnetic stirrer until the solution is uniform and transparent to obtain solution C.

[0048] S05, transfer solutions A, B, and C to a beaker for mixing, and add 10 mL of gentamycin solution prepared in step S01 respectively, and stir evenly;

[0049] S06, replenish the total volume of the solution to 1 L, adjust the pH of the solution to 7.2 with 1 mol / ...

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Abstract

The invention provides a preserving fluid and a preparation method therefor. The preserving fluid is used for preserving umbilical cords and/or placentas. The preserving fluid comprises 50-60g/L of dextran, 6-7g/L of sodium chloride, 200-250mg/L of calcium chloride, 200-400mg/L of potassium chloride, 1-1.2g/L of glucose and 10-30g/L of human serum albumin. The invention further discloses a preparation method for the preserving fluid. Compared with a conventional preserving fluid, the preserving fluid disclosed by the invention reduces the metabolism amount of umbilical cord tissue cells in a process of preserving the umbilical cords, and also can provide certain basic nutrient substances and energy substances to keep a low metabolism level, so that the cell activity is in a stable state, and the conditions such as shape variation or differentiation due to growth and cultivation are avoided; and moreover, the dextran in the components has an anticoagulation effect and can prevent the blood agglutination, so that the interference on the follow-up separation operation is avoided.

Description

technical field [0001] The invention belongs to the technical field of stem cell preparation, and in particular relates to a preservation solution for preserving umbilical cord and / or placenta and a preparation method thereof. Background technique [0002] According to the source, mesenchymal stem cells (mesenchymal stem cells, MSC) include bone marrow mesenchymal stem cells, umbilical cord mesenchymal stem cells and placental mesenchymal stem cells. Compared with MSCs derived from bone marrow, MSCs isolated from placenta or umbilical cord can avoid the traumatic operation of collecting bone marrow, and the in vitro isolation and culture during the collection process are relatively simple, with strong proliferation and differentiation potential and low immunogenicity , Stable biological performance, so it has higher application value. [0003] In order to ensure the properties and cell viability of the obtained umbilical cord MSCs, it is usually prepared from fresh placenta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 曾宪卓鲁菲
Owner SHEN ZHEN ISTEM REGENERATIVE MEDICINE SCI TECH CO LTD
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