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Coating intravascular stent with chitosan carrying siRNA and preparation method of coating intravascular stent

A vascular stent and chitosan technology, applied in the field of vascular stents, can solve the problems of poor water solubility, low transfection efficiency, poor targeting, etc., to prevent thrombus and recurrence, induce cell apoptosis, and treat cell proliferation diseases Effect

Inactive Publication Date: 2015-11-11
李健 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention provides a chitosan-carrying siRNA-coated vascular stent and a preparation method thereof, which solves the problems of low transfection efficiency and poor water solubility of the vascular stent in the prior art, which are easily formed in the stent, restenosis and its coating carrier and poor targeting

Method used

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  • Coating intravascular stent with chitosan carrying siRNA and preparation method of coating intravascular stent
  • Coating intravascular stent with chitosan carrying siRNA and preparation method of coating intravascular stent
  • Coating intravascular stent with chitosan carrying siRNA and preparation method of coating intravascular stent

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preparation example Construction

[0036] A kind of preparation method that chitosan carries siRNA coating vascular stent, comprises the following steps:

[0037] (1) Construction of TF-siRNA target sequence: TF-mRNA167-186bp sequence, the sequence is 5-GCGCTTCAGGCACTACAAAT-3, labeled with fluorescein to obtain TF-siRNA tissue factor;

[0038] (2) Preparation of hydroxybutyl chitosan-carried tissue factor siRNA nanoparticles:

[0039] (a) Take the layered sample of hydroxybutyl chitosan, tear it into thin slices with clean tweezers, put it in a flat glass petri dish, put it under the ultraviolet lamp and irradiate the front and back sides for 50-80 minutes, then dissolve it in acetic acid solution and filter Sterilize to get hydroxybutyl chitosan acetic acid solution;

[0040] (b) Take sodium tripolyphosphate (TPP), add water to dissolve, stir evenly, filter and sterilize to obtain TPP aqueous solution;

[0041] (c) Take the TF-siRNA tissue factor obtained in step (1) and add it to DEPC (diethylpyrocarbonate) w...

Embodiment 1

[0051] A preparation method of hydroxybutyl chitosan carrying tissue factor siRNA coating vascular stent, comprising the following steps:

[0052] (1) Construction of the TF-siRNA target sequence: First, search for the full-length 2104bp mRNA sequence of coagulation factor III (TF) in NCBI (M16553.1, GI: 339503); the TF-siRNA target sequence screened in this experiment was selected for The 167-186bp sequence of TF-mRNA (5-GCGCTTCAGGCACTACAAAT-3) was handed over to the company to design its silencing sequence and labeled with FAM fluorescence to obtain TF-siRNA tissue factor.

[0053] (2) Preparation of hydroxybutyl chitosan-carried tissue factor siRNA nanoparticles:

[0054] (a) Accurately weigh 3 mg of dry hydroxybutyl chitosan sheet-like sample, tear it into thin slices with clean tweezers, lay it flat on a glass petri dish, place it under a UV lamp for 60 minutes on the front and back sides, and then dissolve it in 3 mL Acetic acid solution (concentration of acetic acid is...

Embodiment 2

[0062] A kind of preparation method that chitosan carries siRNA coating vascular stent, comprises the following steps:

[0063] (1) Construction of the TF-siRNA target sequence: First, search for the full-length 2104bp mRNA sequence of coagulation factor III (TF) in NCBI (M16553.1, GI: 339503); the TF-siRNA target sequence screened in this experiment was selected for The TF-mRNA167-186bp sequence (5-GCGCTTCAGGCACTACAAAT-3) was handed over to the company to design its silencing sequence and labeled with fluorescein to obtain TF-siRNA tissue factor.

[0064] (2) Preparation of hydroxybutyl chitosan-carried tissue factor siRNA nanoparticles:

[0065] (a) Accurately weigh 1.5 mg of dry hydroxybutyl chitosan sheet-like sample, tear it into thin slices with clean tweezers, lay it flat on a glass petri dish, place it under a UV lamp for 50 minutes on the front and back sides, and then dissolve it in 3mL acetic acid solution (the concentration of acetic acid is 0.2M, the pH value is ...

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Abstract

The invention discloses a coating intravascular stent with chitosan carrying siRNA and a preparation method of the coating intravascular stent and belongs to the technical field of intravascular stents. The intravascular stent comprises a stent body. A nanometer coating is arranged on the outer surface of the stent body. The nanometer coating is a nanometer coating with the hydroxylbutyl chitosan carrying the tissue factor siRNA. The invention further provides the preparation method of the intravascular stent. The preparation method includes the steps that the tissue factor siRNA is constructed, hydroxylbutyl chitosan nanometer particles carrying the tissue factor siRNA are prepared, alkali treatment is performed on the surface of a bare metal intravascular stent, and the nanometer coating with the hydroxylbutyl chitosan carrying the tissue factor siRNA is prepared. According to the preparation method, the hydroxylbutyl chitosan is made into nanometer particle compounds through the nanometer technology, the hydroxylbutyl chitosan nanometer compounds carrying the tissue factor siRNA are attached to the intravascular stent, RNA genes are quiet by releasing tissue factors, excessive hyperplasia of smooth muscle cells is inhibited, and stent thrombosis and restenosis are prevented.

Description

technical field [0001] The invention belongs to the technical field of vascular stents, in particular to a hydroxybutyl chitosan-carrying tissue factor siRNA-coated vascular stent and a preparation method thereof. Background technique [0002] After coronary artery stenting, it stimulates the excessive proliferation and migration of vascular smooth muscle cells (VSMC), stimulates the expression of local tissue factor in the body to increase significantly, makes the body in a state of high thrombus load, and easily forms in-stent thrombosis and in-stent restenosis. For hyperproliferation of vascular smooth muscle cells (VSMCs), local direct drug delivery strategies, such as drug-eluting stents (DES), can be used to reduce the incidence of restenosis. At the same time, vascular endothelial cells are inhibited to varying degrees. During this period, smooth muscle cells (SMC) are prone to excessive proliferation, migration, and release of tissue factor, causing platelet aggregat...

Claims

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Application Information

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IPC IPC(8): A61L31/10A61L31/16
Inventor 李健李丹安毅万康
Owner 李健
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