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Nano-sensor for colorimetric/fluorescence dual-mode rapid detection of antibiotics and antibiotic detection method

A nano-sensor and antibiotic technology, applied in the fields of biomedicine and nano-medicine, can solve the problems of timely monitoring of drug residues, inability to screen samples at the same time, etc., to reduce detection concentration and detection limit, improve detection reliability, and make a good choice. sexual effect

Pending Publication Date: 2015-11-11
惠州出入境检验检疫局检验检疫综合技术中心 +2
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Problems solved by technology

Most of these methods require complex and cumbersome sample pretreatment, and cannot screen a large number of samples at the same time, which is not suitable for timely monitoring of drug residues

Method used

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  • Nano-sensor for colorimetric/fluorescence dual-mode rapid detection of antibiotics and antibiotic detection method
  • Nano-sensor for colorimetric/fluorescence dual-mode rapid detection of antibiotics and antibiotic detection method

Examples

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Embodiment 1

[0030] Preparation of nanosensors for the measurement of chloramphenicol and a colorimetric / fluorometric dual-modal rapid detection of antibiotics:

[0031] (1) Preparation of gold nanoparticles (AuNPs) with a diameter of 13nm: weigh 0.04726gHAuCl 4 Dissolve in a round-bottomed flask with 100mL of ultrapure water, put a magnetic stirrer, then connect a condenser tube and a stopper to the two ports of the two-necked flask, turn on the condensate, turn on the magnetic stirrer and start heating. In addition, 1.3693 g of trisodium citrate was dissolved in ultrapure water, and configured into a 120 mL solution to obtain a 38.8 mM trisodium citrate solution. When the reaction solution boils and the condensed water begins to flow back at a rate of 1 drop per second, remove the stopper, quickly add 12 mL of 38.8 mM trisodium citrate, and re-plug the stopper. At this time, the color of the solution will gradually change from light yellow to dark red, and then continue to heat and refl...

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Abstract

The invention provides a nano-sensor for colorimetric / fluorescence dual-mode rapid detection of antibiotics. The nano-sensor includes gold nanoparticles, by means of electrostatic adsorption, the surfaces of the gold nanoparticles are adsorbed with aptamer able to specifically bind with specific antibiotic, and the aptamer is marked with a fluorescent dye. The invention aims to provide the nano-sensor for colorimetric / fluorescence dual-mode rapid detection of antibiotics with short detection time and high detection precision, and also provides a method for colorimetric / fluorescence dual-mode rapid detection of antibiotics.

Description

technical field [0001] The invention relates to the fields of biomedicine and nanomedicine, more specifically, to a nanometer sensor for rapidly detecting antibiotics in a colorimetric / fluorescent dual mode, and a method for rapidly detecting antibiotics in a colorimetric / fluorescent dual mode. Background technique [0002] Aptamers are a class of RNA or DNA single-stranded oligonucleotides that can specifically bind to target molecules and are screened out using the SELEX system. It has high affinity and specificity, does not depend on the existence of organisms, and can specifically bind to many substances such as metal ions and amino acids. When combined with the target, the tertiary structure of the nucleic acid aptamer changes, transforming into a hairpin structure, pocket structure, G-tetrad structure, etc., and its adsorption properties, steric hindrance, etc. also change. The use of gold nanoparticles can convert these signals into signals that can be recognized by ...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N21/33G01N21/64
Inventor 吕飞熊贝贝谢堂堂
Owner 惠州出入境检验检疫局检验检疫综合技术中心
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