Detection method of quinolones residue in animal tissue

A quinolone and detection method technology, which is applied in the field of detection of quinolone drug residues in animal tissues, can solve the problems of rapid detection of unfavorable samples, long time consumption, etc., achieves good recovery rate and reduces the effect of matrix effect

Inactive Publication Date: 2015-11-11
威海出入境检验检疫局检验检疫技术中心
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Problems solved by technology

However, it takes a long time to process samples by membrane dialysis (dialysis time ≥ 6h), which is not conducive to the rapid detection of samples

Method used

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  • Detection method of quinolones residue in animal tissue
  • Detection method of quinolones residue in animal tissue
  • Detection method of quinolones residue in animal tissue

Examples

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Embodiment Construction

[0013] 1. Sample pretreatment

[0014] Accurately weigh 5.00g of crushed sample, add 20mL of 1% acetic acid to acidify acetonitrile, mix well, ultrasonicate for 20min, add 5g of baked anhydrous sodium sulfate and mix well, centrifuge at 8000r / min for 5min, take about 12mL supernatant Slowly add to the ultrafiltration tube along the tube wall, centrifuge at 5000r / min for 10min, take out 10mL of the centrifuged liquid and dry it with nitrogen gas, and dilute the mobile phase to 1.0mL for HPLC-MS / MS analysis.

[0015] 2. Liquid chromatography conditions

[0016] The chromatographic column used in this example is WatersXbridgeC18, 3.5μm2.1×150mm, Agilent ZorbaxSB-C18, XDB-C18 and other octadecyl bonded phase silica gel can also meet the separation requirements.

[0017] Injection volume: 20 μL; mobile phase: A: 0.1% formic acid solution; B: acetonitrile; flow rate: 0.2 mL / min; see Table 1 for gradient elution conditions.

[0018] Table 1 Liquid Chromatography Gradient Elution ...

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Abstract

The invention discloses a detection method of quinolones residue in animal tissue. The detection method comprises following steps: tissue samples to be tested are subjected to pretreatment; a supernate is obtained via extraction, and is delivered into ultrafiltration tubes; the ultrafiltration tubes are delivered into a centrifugal machine for centrifugation; a liquid obtained via centrifugation is dried with nitrogen flow; a mobile phase is used for dilution; and instrument measurement analysis is carried out. The detection method can be used for simultaneous detection of ten quinolones residue, and is high in recovery rate, stability, and repeatability. Macromolecular impurities are intercepted, so that matrix effects are reduced greatly. The detection method is capable of satisfying daily detection requirements of ten quinolones in animal source food.

Description

technical field [0001] The invention belongs to the field of detection of veterinary drug residues, in particular to a detection method for quinolone drug residues in animal tissues. Background technique [0002] Quinolones (QNs) drugs are a class of synthetic antibacterial drugs with strong antibacterial effect and broad antibacterial spectrum, which are widely used in clinical diagnosis, animal disease prevention and growth promotion. Excessive or inappropriate use of QNs in animal-derived foods will cause potential "three-cause" (carcinogenic, teratogenic, and mutagenic) effects in consumers, induce drug resistance in bacteria, and threaten human health. Therefore, many countries and organizations restrict its use and formulate corresponding maximum residue limits (MRLs): the United States prohibits the use of FQNs in food animal breeding; the only QNs approved for use in Japan are enrofloxacin, difloxacin, a Bifloxacin, dafloxacin, marbofloxacin, ofloxacin, and oxolinic...

Claims

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Application Information

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IPC IPC(8): G01N30/06
Inventor 李兆杰王静鞠玲燕胡巧茹杨丽君
Owner 威海出入境检验检疫局检验检疫技术中心
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