Surface plasma resonator chip based on modification of hyaluronic acid assisted by dopamine and preparation method of chip

A hyaluronic acid modification, surface plasma technology, applied in the direction of instruments, scientific instruments, material testing products, etc., can solve the problems of low efficiency, cumbersome operation, long time consumption, etc., to achieve simple operation, improved surface hydrophilicity, utilization of rate increase effect

Active Publication Date: 2015-11-11
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, in the modification process of carboxymethyl dextran sensor chip, the operation is cumbersome, time-consuming (more than 12h), strong corrosive substances (bromoacetic acid) are used, and the efficiency is low

Method used

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  • Surface plasma resonator chip based on modification of hyaluronic acid assisted by dopamine and preparation method of chip
  • Surface plasma resonator chip based on modification of hyaluronic acid assisted by dopamine and preparation method of chip
  • Surface plasma resonator chip based on modification of hyaluronic acid assisted by dopamine and preparation method of chip

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Embodiment 1

[0037] A 2 nm thick chromium layer was first coated on the BK7 glass substrate by vacuum sputtering, and then a 45 nm gold film was coated to obtain a bare gold chip.

Embodiment 2

[0039] A 2 nm thick chromium layer was first coated on the BK7 glass substrate by vacuum sputtering, and then a 48 nm gold film was coated to obtain a bare gold chip.

Embodiment 3

[0041] A 2 nm thick chromium layer was first coated on the BK7 glass substrate by vacuum sputtering, and then a 50 nm gold film was coated to obtain a bare gold chip.

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Abstract

The invention relates to a surface plasma resonator chip based on modification of hyaluronic acid assisted by dopamine, and a preparation method of the chip. The chip comprises a structure consisting of a glass layer, a chromium layer, a sensing layer, and a dopamine and hyaluronic acid mixed layer in sequence. The preparation method comprises: firstly, coating a BK7 glass substrate with the chromium layer through vacuum sputtering, and then coating the chromium layer with the sensing layer to obtain a sensing chip; immersing the sensing chip in a solution mixed by concentrated sulfuric acid and hydrogen peroxide; and immersing the obtained chip into a hyaluronic acid solution of dopamine to form the dopamine and hyaluronic acid mixed layer. The surface plasma resonator chip based on hyaluronic acid is high in stability, and is unchangeable in pollution resistance after stored for 4 months in a dry state or for 7 days in a phosphate buffer with the pH of 7.4. The chip can be recyclable for reuse, and after ten times recycling, the chip can also keep good pollution resistance. The preparation method is simple in operation and has good economic practicability.

Description

technical field [0001] The invention relates to a surface plasmon resonance chip based on dopamine-assisted hyaluronic acid modification and a preparation method thereof, which belongs to the design and preparation method of an anti-pollution chip of a surface plasmon resonance spectrometer, and is widely used in medical testing, environmental science, food safety, etc. field. Background technique [0002] Surface contamination is a major problem to be solved in medical diagnosis, environmental monitoring, organ transplantation and other industries, especially in the field of biosensing and detection. In SPR sensing detection, with the continuous complexity of the detection system, non-specific substances in the solution to be detected will be adsorbed on the surface of the chip, which will not only reduce the performance of the diagnostic instrument, but also cause "false positive" detection results. ". Therefore, the development of biosensing chips with excellent anti-po...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N21/553
Inventor 苏荣欣黄仁亮刘霞齐崴
Owner TIANJIN UNIV
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