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Blood compatible polymer layer and preparation method thereof

A technology of blood compatibility and polymer layer, which is applied in the fields of coating, surgery, medical science, etc., can solve the problem that the hydrophobic property does not achieve the anti-fouling effect, and achieve excellent anti-red blood cell adhesion performance, controllable experimental conditions, Excellent anti-platelet adhesion effect

Active Publication Date: 2021-04-02
JIAXING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although it has been reported that AACA monomers have been successfully used to construct blood-compatible polymer layers (Colloids and Surfaces B: Biointerfaces, 2018, 161, 73-82, DOI: 10.1016 / j.colsurfb.2017.10.035), but The strong hydrophobic properties of the monomer itself did not achieve a very ideal antifouling effect

Method used

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  • Blood compatible polymer layer and preparation method thereof
  • Blood compatible polymer layer and preparation method thereof
  • Blood compatible polymer layer and preparation method thereof

Examples

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Embodiment 1

[0047] A method for preparing a blood-compatible polymer layer. Under the condition of ultraviolet light irradiation, the copolymerization reaction of 6-acrylamidocaproic acid and N-vinylpyrrolidone is initiated on the surface of a silanized silicon wafer, and the grafted polymer layer is obtained. Hemocompatible polymer layers on silanized samples, such as Figure 9 As shown, the specific steps are as follows:

[0048] (1) Place the silicon chip in mixed solution I (a mixed solution of concentrated sulfuric acid and hydrogen peroxide, concentrated sulfuric acid is a sulfuric acid solution with a mass fraction of 90%, and hydrogen peroxide is a hydrogen peroxide with a volume fraction of 30%), and heat at 80°C After taking it out for 30 minutes, clean the surface with deionized water, blow dry with high-purity nitrogen (purity 99.999%), and obtain a silicon wafer with surface hydroxylation, named Si-OH, and denoted as A; where concentrated sulfuric acid and hydrogen peroxide ...

Embodiment 2

[0067] A preparation method of a blood-compatible polymer layer, the specific steps are as follows:

[0068] (1) Place the substrate (ceramic) in mixed solution I (a mixed solution of concentrated sulfuric acid and hydrogen peroxide, concentrated sulfuric acid is a sulfuric acid solution with a mass fraction of 98%, and hydrogen peroxide is a hydrogen peroxide with a volume fraction of 30%), and heat Heating at lower temperature for 30min, cleaning the surface with deionized water after taking it out, and drying it with high-purity nitrogen gas (purity 99.999%) to obtain the substrate of surface hydroxylation; the volume ratio of concentrated sulfuric acid and hydrogen peroxide in the mixed solution I is 3: 1;

[0069] (2) carrying out silanization treatment to the surface hydroxylated substrate obtained in step (1) to obtain a silanized substrate;

[0070] The process of specific silanization treatment is: the surface hydroxylated substrate obtained in step (1) is placed in ...

Embodiment 3

[0075] A preparation method of a blood-compatible polymer layer, the specific steps are as follows:

[0076] (1) Put the substrate (glass) in the mixed solution I (a mixed solution of concentrated sulfuric acid and hydrogen peroxide, where the concentrated sulfuric acid is a sulfuric acid solution with a mass fraction of 98%, and the hydrogen peroxide is a hydrogen peroxide with a volume fraction of 30%). After heating for 32min, take it out, clean the surface with deionized water, and dry it with high-purity nitrogen gas (purity 99.999%) to obtain a hydroxylated substrate on the surface; the volume ratio of concentrated sulfuric acid and hydrogen peroxide in the mixed solution I is 3: 1;

[0077] (2) carrying out silanization treatment to the surface hydroxylated substrate obtained in step (1) to obtain a silanized substrate;

[0078] The process of specific silanization treatment is: the surface hydroxylated substrate obtained in step (1) is placed in mixed solution II (sil...

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Abstract

The invention relates to a blood compatible polymer layer and a preparation method thereof. Under the condition of ultraviolet irradiation, 6-acrylamide aminocaproic acid and N-vinyl pyrrolidone are initiated to be subjected to a copolymerization reaction on the surface of a silanized substrate, and the blood compatible polymer layer is prepared; and the water contact angle of the prepared blood compatible polymer layer is 40-45 degrees. After the surface of the silanized substrate is grafted with the blood compatible polymer layer, the BSA and Fib protein adsorption amounts are respectively reduced by 75-80% and 60-68%, and no obvious adhesion phenomenon is observed on an erythrocyte adhesion test picture and a platelet adhesion test picture by adopting a laser confocal microscopy test. According to the invention, ultraviolet light is utilized to initiate copolymerization of AACA and NVP monomers, the blood compatible polymer layer with hydrophilic-hydrophobic balance capability is introduced to the surface of the substrate, the operation is simple, the reaction process is controllable, the method is clean and pollution-free, and large-scale production can be realized; and the prepared blood compatible polymer layer has excellent protein adsorption resistance, erythrocyte adhesion resistance and platelet adhesion resistance.

Description

technical field [0001] The invention belongs to the technical field of medical biomaterials, and relates to a blood-compatible polymer layer and a preparation method thereof. Background technique [0002] In recent years, with the rapid development of economy and science and technology, people's demand for biomedical materials is increasing, especially artificial blood vessels, tissue scaffolds, bionic joints, etc. However, due to the surface non-polar and hydrophobic characteristics of biological materials such as general-purpose polymer materials, metal materials, and ceramics, protein adsorption, coagulation, and hemolysis often occur after contact with blood, resulting in acute toxicity, inflammation, teratogenicity, and Foreign body rejection and other reactions severely limit the application in the field of biomedicine. Therefore, the development of blood-compatible medical biomaterials has application value and social significance. [0003] Polymer materials have be...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F292/00C08F220/58C08F226/10A61L31/10A61L31/14
CPCC08F292/00A61L31/10A61L31/14C08F220/58C08F226/10
Inventor 杜艳秋姜旸李义代正伟
Owner JIAXING UNIV
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