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A temperature-sensitive regenerable capillary, its preparation method and application

A temperature-sensitive, capillary technology, applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve the problems of complex coating process and difficult regeneration of capillary wall coating materials, and achieve weak anti-protein adsorption ability and anti-protein adsorption ability strong effect

Active Publication Date: 2019-06-25
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] For this reason, the technical problem to be solved by the present invention lies in the problem that the existing capillary wall coating material coating process is complex and difficult to regenerate, and then provide a temperature-sensitive regenerative capillary, which can pass through the layer The layer self-assembly method is carried out in the aqueous phase, and the preparation process is simple and fast

Method used

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  • A temperature-sensitive regenerable capillary, its preparation method and application
  • A temperature-sensitive regenerable capillary, its preparation method and application
  • A temperature-sensitive regenerable capillary, its preparation method and application

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preparation example Construction

[0037] The preparation method of the above-mentioned temperature-sensitive regenerative capillary is as follows: image 3 shown, including the following steps:

[0038] S0, capillary internal activation pretreatment

[0039] Concentrations of 0.09-0.11mol·L were used successively -1 Sodium hydroxide solution, water, the concentration is 0.09-0.11mol L -1 Flush the capillary with hydrochloric acid solution, water and methanol for 25-35min, 8-12min, 25-35min, 8-12min and 8-12min respectively, and then dry the capillary with inert gas to obtain an activated capillary.

[0040] S1, the concentration is 2.0mg·ml -1 -4.0mg·ml -1 The photosensitive diazo resin solution, the temperature-sensitive polyethylene glycol solution and water with a concentration of 4.0mg·ml-1-8.0mg·ml-1 are slowly injected into the capillary, and repeated 2-8 times; the photosensitive diazo The mass ratio of the resin to the temperature-sensitive polyethylene glycol is 1:1-1:2, the weight-average molecu...

Embodiment 1

[0051] A temperature-sensitive regenerable capillary, the inner wall of the capillary is modified with a regenerable anti-protein adsorption covalently bonded coating formed by a temperature-sensitive high molecular polymer material, and the regenerable anti-protein adsorption covalently bonded coating It is in a stretched or contracted state as the temperature changes, so that the pore size of the capillary changes with the temperature change. The temperature-sensitive polymer material layer is a regenerative anti-protein adsorption covalently bonded coating formed by diazo resin and temperature-sensitive polyethylene glycol. When the temperature is lower than the formed regenerative anti-protein adsorption covalently When the temperature is lower than the lower critical solution temperature of the valence bonded coating, the polymer material layer is in a stretched state; when the temperature is higher than the lower critical solution temperature of the formed regenerative an...

Embodiment 2

[0063] The structure of the temperature-sensitive regenerable capillary of this embodiment is the same as that of the embodiment, and its preparation method includes the following steps:

[0064] S0, capillary internal activation pretreatment

[0065] successively with a concentration of 0.09mol·L -1 Sodium hydroxide solution, distilled water, the concentration is 0.11mol L -1 Flush the capillary with hydrochloric acid solution, distilled water and methanol for 35min, 8min, 35min, 8min and 8min respectively, and then use N 2 The activated capillary can be obtained by drying the capillary.

[0066] S1, the concentration is 3.0mg·ml -1 8ml of photosensitive diazo resin solution, 2ml of distilled water, the concentration is 4.0mg·ml -1 8ml of temperature-sensitive polyethylene glycol solution and 2ml of distilled water are slowly injected into the capillary successively, thus completing an electrostatic self-assembly cycle; the mass ratio of the photosensitive diazo resin to ...

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Abstract

The invention designs a thermo-sensitive regenerative capillary tube. A regenerative protein adsorption-resistant covalent bonding coating formed by a thermo-sensitive macromolecular polymer is modified on the inner wall of the capillary tube, and the regenerative protein adsorption-resistant covalent bonding coating is in an extended or contraction state along with variation of temperature, so that the pore size of the capillary tube is changed along with the variation of the temperature; the thermo-sensitive regenerative capillary tube can be obtained in an aqueous phase in a layer-by-layer self-assembly way, so that preparation process is simple and quick. Chemical bonding of the coating is realized by carrying out photocuring crosslinking reaction on sulfydryl of a thermo-sensitive polyethylene glycol polymer and silicon hydroxyl on the inner wall of the capillary tube by virtue of photosensitive diazoresin, the coating is stable, and the coating can be regenerated by adopting a temperature regulating way.

Description

technical field [0001] The invention relates to the field of regenerable capillary tubes, in particular to a temperature-sensitive regenerable capillary tube and a method for preparing and using the capillary tube. Background technique [0002] In recent years, capillary electrophoresis has been widely used in the fields of drug detection, life science, environmental protection and food inspection, especially in various It has been rapidly developed and widely used in the separation and analysis of biological macromolecules such as proteins and RNA. However, due to the electrostatic interaction, van der Waals force and hydrogen bond interaction between the unmodified capillary inner wall surface and the protein, non-specific adsorption occurs on the inner wall of the capillary, which affects the separation and analysis of proteins in complex systems. At present, coating the inner wall of the capillary is the most effective and commonly used method to improve the separation ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/447
CPCG01N27/447G01N27/44704
Inventor 丛海林于冰陈新
Owner QINGDAO UNIV
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