Enzymatic pretreatment assisted curcumin extraction technology
A technology of auxiliary extraction and curcumin, applied in the separation/purification of carbonyl compounds, organic chemistry and other directions, can solve the problems of curcumin extract that is not easy to dry, destroy curcumin activity, accelerate curcumin dissolution, etc., and achieve easy control of reaction conditions. , The effect of improving clarity and stable product quality
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[0032]
[0033] A process for the auxiliary extraction of curcumin by enzymatic pretreatment, which comprises the following steps:
[0034] Step 1: Take 1 part of turmeric crushed and sieved to 60 mesh, then add 3 parts of tap water and stir evenly, seal the water bath at 60°C for 60min, stir 2 times in the water bath, then cool down and keep constant to 52°C to obtain turmeric slurry;
[0035] Step 2: Add a complex enzyme consisting of 8U / g turmeric cellulase, 60U / g turmeric amylase and 5U / g turmeric papain to the turmeric slurry, and react for 2h under pH 3.8 to obtain the enzyme Solution
[0036] Step 3: Take the enzymatic hydrolysis solution described in Step 2, and add 7 times its volume multiple of ethanol solution, homogenize for 1 min under the condition of 8000r / min to obtain curcumin extract, wherein the mass fraction of the ethanol solution is 65%;
[0037] Step 4: Add 0.3g / L of chitosan to the extract and stir for 30 minutes, filter, and pass the obtained clear liquid thro...
Example Embodiment
[0038]
[0039] A process for the auxiliary extraction of curcumin by enzymatic pretreatment, which comprises the following steps:
[0040] Step 1: Take 1 part of turmeric crushed and sieved to 80 mesh, then add 6 parts of tap water and stir well, seal the water bath at 70°C for 60min, stir 3 times in the water bath, then cool down and keep it constant to 58°C to obtain a turmeric slurry;
[0041] Step 2: Add a complex enzyme composed of 12U / g turmeric cellulase, 90U / g turmeric amylase and 10U / g turmeric papain to the turmeric slurry, and react for 4h under pH 4.5 to obtain the enzyme Solution
[0042] Step 3: Take the enzymatic hydrolysis solution described in Step 2, add 9 times its volume multiple of ethanol solution, homogenize for 2 min under the condition of 9000r / min to obtain curcumin extract, wherein the mass fraction of the ethanol solution is 70%;
[0043] Step 4: Add 0.5g / L of sodium polyacrylate to the extract and stir for 60 minutes, then filter, and pass the obtained cl...
Example Embodiment
[0044]
[0045] A process for the auxiliary extraction of curcumin by enzymatic pretreatment, which comprises the following steps:
[0046] Step 1: Take 1 part of turmeric crushed and sieved to 70 mesh, then add 5 parts of tap water and stir evenly, seal the water bath at 65℃ for 60min, stir 3 times in the water bath, then cool down and keep constant to 55℃ to obtain turmeric slurry;
[0047] Step 2: Add a complex enzyme composed of 10 U / g turmeric cellulase, 80 U / g turmeric amylase and 7 U / g turmeric papain to the turmeric slurry, and react for 3 hours under pH 4.0 to obtain the enzyme Solution
[0048] Step 3: Take the enzymatic hydrolysate described in Step 2, add 8 times its volume multiple of ethanol solution, homogenize for 1.5 min under the condition of 8500r / min to obtain curcumin extract, wherein the mass fraction of the ethanol solution is 68%;
[0049] Step 4: Add 0.5g / L of polyacrylamide to the extract and stir for 50 minutes, filter, and pass the clear liquid through an a...
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