Enzymatic pretreatment assisted curcumin extraction technology

A technology of auxiliary extraction and curcumin, applied in the separation/purification of carbonyl compounds, organic chemistry and other directions, can solve the problems of curcumin extract that is not easy to dry, destroy curcumin activity, accelerate curcumin dissolution, etc., and achieve easy control of reaction conditions. , The effect of improving clarity and stable product quality

Inactive Publication Date: 2015-11-18
BAOTOU MEDICAL COLLEGE OF INNER MONGOLIA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in order to increase the extraction efficiency and accelerate the stripping of curcumin, the organic solvent extraction usually needs to increase the extraction temperatu

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0032]

[0033] A process for the auxiliary extraction of curcumin by enzymatic pretreatment, which comprises the following steps:

[0034] Step 1: Take 1 part of turmeric crushed and sieved to 60 mesh, then add 3 parts of tap water and stir evenly, seal the water bath at 60°C for 60min, stir 2 times in the water bath, then cool down and keep constant to 52°C to obtain turmeric slurry;

[0035] Step 2: Add a complex enzyme consisting of 8U / g turmeric cellulase, 60U / g turmeric amylase and 5U / g turmeric papain to the turmeric slurry, and react for 2h under pH 3.8 to obtain the enzyme Solution

[0036] Step 3: Take the enzymatic hydrolysis solution described in Step 2, and add 7 times its volume multiple of ethanol solution, homogenize for 1 min under the condition of 8000r / min to obtain curcumin extract, wherein the mass fraction of the ethanol solution is 65%;

[0037] Step 4: Add 0.3g / L of chitosan to the extract and stir for 30 minutes, filter, and pass the obtained clear liquid thro...

Example Embodiment

[0038]

[0039] A process for the auxiliary extraction of curcumin by enzymatic pretreatment, which comprises the following steps:

[0040] Step 1: Take 1 part of turmeric crushed and sieved to 80 mesh, then add 6 parts of tap water and stir well, seal the water bath at 70°C for 60min, stir 3 times in the water bath, then cool down and keep it constant to 58°C to obtain a turmeric slurry;

[0041] Step 2: Add a complex enzyme composed of 12U / g turmeric cellulase, 90U / g turmeric amylase and 10U / g turmeric papain to the turmeric slurry, and react for 4h under pH 4.5 to obtain the enzyme Solution

[0042] Step 3: Take the enzymatic hydrolysis solution described in Step 2, add 9 times its volume multiple of ethanol solution, homogenize for 2 min under the condition of 9000r / min to obtain curcumin extract, wherein the mass fraction of the ethanol solution is 70%;

[0043] Step 4: Add 0.5g / L of sodium polyacrylate to the extract and stir for 60 minutes, then filter, and pass the obtained cl...

Example Embodiment

[0044]

[0045] A process for the auxiliary extraction of curcumin by enzymatic pretreatment, which comprises the following steps:

[0046] Step 1: Take 1 part of turmeric crushed and sieved to 70 mesh, then add 5 parts of tap water and stir evenly, seal the water bath at 65℃ for 60min, stir 3 times in the water bath, then cool down and keep constant to 55℃ to obtain turmeric slurry;

[0047] Step 2: Add a complex enzyme composed of 10 U / g turmeric cellulase, 80 U / g turmeric amylase and 7 U / g turmeric papain to the turmeric slurry, and react for 3 hours under pH 4.0 to obtain the enzyme Solution

[0048] Step 3: Take the enzymatic hydrolysate described in Step 2, add 8 times its volume multiple of ethanol solution, homogenize for 1.5 min under the condition of 8500r / min to obtain curcumin extract, wherein the mass fraction of the ethanol solution is 68%;

[0049] Step 4: Add 0.5g / L of polyacrylamide to the extract and stir for 50 minutes, filter, and pass the clear liquid through an a...

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Abstract

The invention provides an enzymatic pretreatment assisted curcumin extraction technology. The enzymatic pretreatment assisted curcumin extraction technology comprises steps as follows: after 1 part of curcuma powder is taken, ground, sieved, added with water and stirred uniformly, the mixture is subjected to water bath for 60 min and is cooled to the constant temperature of 52-58 DEG C, and curcuma pulp is obtained; compound enzyme comprising cellulase, amylase and papain is added to the curcuma pulp and reacts for 2-4 h, and enzymatic hydrolysate is obtained; the enzymatic hydrolysate is taken, added with an ethanol solution and homogenized for 1-2 min under the condition of 8,000-9,000 r/min, and a curcumin extracting solution is obtained; finally, a flocculant is added to the extracting solution, the mixture is stirred and filtered, a clear liquid flows through an activated carbon chromatographic column and is eluted with an alkaline solution, and a curcumin desorbed solution is obtained. The technology has the advantages of high extraction speed, low temperature, low energy consumption, high yield of the effective component curcumin, stable product quality and controllable reaction conditions, and is applicable to industrial large-scale production.

Description

technical field [0001] The invention relates to an extraction process of curcumin. More specifically, the present invention relates to a process for assisting the extraction of curcumin by enzymatic pretreatment, which can effectively improve the extraction efficiency and purity of curcumin. Background technique [0002] Curcumin is extracted from turmeric, a traditional Chinese medicine, and is the most important component of turmeric to exert its pharmacological effects. Curcumin can promote the formation of immune cells called macrophages, which can clear a type of amyloid that can form Alzheimer's-related, and has excellent preventive effects on Alzheimer's disease. In addition, curcumin also has physiological and pharmacological effects such as promoting gallbladder and protecting liver, lowering blood fat, promoting blood circulation and promoting menstrual flow, anti-HIV, anti-breast cancer, anti-prostate cancer, and anti-atherosclerosis. [0003] There are many rep...

Claims

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Application Information

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IPC IPC(8): C07C45/78C07C49/255C07C45/79
CPCC07C45/78C07C45/79C07C49/255
Inventor 齐瑞芳高洪波方欣石瑞丽马宝慧吕军陈晓东时静华徐继辉王立军
Owner BAOTOU MEDICAL COLLEGE OF INNER MONGOLIA UNIV OF SCI & TECH
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