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Aureomycin fermentation culture medium and aureomycin fermentation production method by using same

A fermentation medium and chlortetracycline technology, which is applied in the field of chlortetracycline preparation, can solve the problems of high price of soybean cake flour, influence on chlortetracycline fermentation production effect, lack of innovation, etc., and achieve stable fermentation quality, good fermentation effect, Excellent fermentation effect

Active Publication Date: 2015-11-18
ZHUMADIAN HUAZHONG CHIA TAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In the existing chlortetracycline fermentation preparation process, soybean cake powder is usually used as a long-term nitrogen source, but due to the high price of soybean cake powder, in the actual production process, it is usually replaced by peanut cake powder, corn flour, etc. The use of soybean cake powder, but as a result of material substitution, it is often necessary to redesign the nutrient composition of the medium to adapt to and obtain the best fermentation effect. Due to the process and stabilization of aureomycin production process, In the actual production process, there is often a lack of innovation in the composition of the optimal medium, which affects the improvement of the production effect of aureomycin fermentation to a certain extent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The aureomycin fermentation medium that present embodiment adopts, the specific composition in every 1L medium is:

[0030] 12g peanut cake powder, 20g soybean cake powder, 100g corn starch, 14g corn syrup,

[0031] Yeast powder 5g, soybean oil 5g, amylase 0.1g,

[0032] Sodium chloride 2.5g, ammonium sulfate 5g, calcium carbonate 7g, magnesium sulfate 0.25g, potassium dihydrogen phosphate 0.17g, pH natural.

[0033] After its improvement, the same amount of sesame cake powder was used to completely replace the soybean cake powder, that is, the substitution rate of sesame cake powder for soybean cake powder was 100%, and other components and formulas were the same.

[0034] After the above medium was sterilized in a conventional manner, a 10L fermenter was used for a small-scale fermentation experiment.

[0035] The complete fermentation process is as described in the description part, and during the brief period, only the seed liquid preparation process is taken as a...

Embodiment 2

[0039] The fermented medium formula that contains soybean cake powder in the present embodiment is the same as embodiment 1, but when adopting sesame cake powder to replace soybean cake powder in the present embodiment, the replacement rate is 50%, that is, every 1L medium contains soybean cake powder 10g, Sesame cake powder 10g.

[0040] The fermentation process of the present embodiment is the same as embodiment 1, but adopted 60m 3 The fermenter was used for actual production experiments.

[0041] After the fermentation, the measurement results showed that the number of bacteria in the fermentation medium containing soybean cake powder (data level is billion level) was 1069, while the number of bacteria in the fermentation medium using sesame cake powder (data level The same billion level) is 1102, which increases the fermentation effect by about 1.5%. The above data showed that the two culture media showed good fermentation effect.

Embodiment 3

[0043] The fermented medium formula that contains soybean cake powder in the present embodiment is the same as embodiment 1, but when adopting sesame cake powder to replace soybean cake powder in the present embodiment, the replacement rate is 90%, that is, every 1L medium contains soybean cake powder 2g, Sesame cake powder 18g.

[0044] The fermentation process of the present embodiment is the same as embodiment 1, but adopted 60m 3 The fermenter was used for actual production experiments.

[0045] After the fermentation, the measurement results showed that the number of bacteria in the fermentation medium containing soybean cake powder (data level is billion level) was 1063, while the number of bacteria in the fermentation medium using sesame cake powder (data level The same billion level) is 1103, which increases the fermentation effect by about 2.4%. The above data showed that the two culture media showed good fermentation effects.

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Abstract

The invention belongs to the technical field of aureomycin preparation, and particularly relates to an aureomycin fermentation culture medium and an aureomycin fermentation production method by using the same. Every 1L of culture medium is composed of 12-22g of peanut meal, 8-20g of soybean cake powder, 50-100g of corn starch, 10-14g of corn steep liquor, 5.0-9.0g of yeast powder, 2.5-5g of bean oil, 0.1-0.2g of amylase, 1.5-3.5g of sodium chloride, 5-8g of ammonium sulfate, 5.6-8.0g of calcium carbonate, 0.1-0.3g of magnesium sulfate and 0.1-0.5g of potassium dihydrogen phosphate. The pH value is natural. The invention provides an optimized aureomycin fermentation culture medium. The sesame meal is partially or completely substituted for the soybean cake powder for further optimization, thereby obtaining favorable fermentation effects.

Description

technical field [0001] The invention belongs to the technical field of aureomycin preparation, and in particular relates to a chlortetracycline fermentation medium and a method for producing chlortetracycline by fermentation using the medium. Background technique [0002] Aureomycin is a commonly used antibiotic additive in the process of livestock and poultry breeding. Its preparation process is generally obtained from Streptomyces aureus as the starting strain through biological fermentation and purification. [0003] In the existing chlortetracycline fermentation preparation process, soybean cake powder is usually used as a long-term nitrogen source, but due to the high price of soybean cake powder, in the actual production process, it is usually replaced by peanut cake powder, corn flour, etc. The use of soybean cake powder, but as a result of material substitution, it is often necessary to redesign the nutrient composition of the medium to adapt to and obtain the best f...

Claims

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Application Information

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IPC IPC(8): C12P29/00C12R1/49
Inventor 李书至白雪原樊春光卜令军
Owner ZHUMADIAN HUAZHONG CHIA TAI
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