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Micro device for enhancing fluorescence of fluorescent molecules

A fluorescent molecule and fluorescence enhancement technology, applied in fluorescence/phosphorescence, material analysis by optical means, instruments, etc., can solve the problems of poor repeatability, experimental test uniformity and repeatability are not very good, and achieve reliability and reliability. Good reproducibility and enhanced fluorescence

Active Publication Date: 2015-11-18
SUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although it is known that the two competing phenomena of enhancement and quenching are inseparable from the distance between molecules and nanoparticles, there is still a lack of devices for fluorescence enhancement of fluorescent molecules in the prior art. There are two methods for the distance between fluorescent molecules: one method is to isolate nanoparticles and fluorescent molecules through light-transmitting materials such as silicon dioxide, and this method often requires precise control of the thickness of silicon dioxide , poor reproducibility; Another method is to mix nanoparticles into organic solvents mixed with fluorescent molecules
Although this method can get a higher fluorescence enhancement factor, the uniformity and repeatability of the experimental test are not very good

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  • Micro device for enhancing fluorescence of fluorescent molecules
  • Micro device for enhancing fluorescence of fluorescent molecules
  • Micro device for enhancing fluorescence of fluorescent molecules

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preparation example Construction

[0033] In this embodiment, the preparation method of the miniature fluorescent molecular fluorescence enhancement device includes the following steps, such as figure 2 As shown, it mainly includes the configuration of seed growth solution, the synthesis of Au rods, the synthesis of AuAg composite nanorod dispersion, the substrate modification, the assembly of AuAg composite nanorods and the spin-coating of PMMA, as follows:

[0034] (a) Configuration of seed growth solution: HAuCl 4(0.01~0.05mol / L, 0.2~0.5ml) solution is dissolved in cetyltrimethylammonium bromide solution (abbreviated as CTAB, 0.1~0.5mol / L, 5~10ml), and then add ice water cooling NaBH 4 Solution (0.01~0.05mol / L, 0.5~1ml), stirred for 2~3 minutes to form seed growth solution;

[0035] (b) Synthesis of Au rods: take CTAB again (since the same solution is selected, its concentration is the same as above, and the volume is 30~50ml), and add HAuCl to it in turn 4 solution (since the same solution is selected, ...

Embodiment 1

[0041] The invention provides a preparation method of a miniature fluorescent molecular fluorescence enhancement device, specifically:

[0042] (1) The configuration of the seed growth solution: add 0.25ml of 0.01M (mol / L) HAuCl 4 Dissolve in 7.5ml0.1MCTAB solution, then add 0.6ml0.01M freshly prepared NaBH 4 (ice water cooling) solution, gently stirred for 2-3 minutes to form a seed growth solution, and the seed growth solution was stored at 27°C for 2-5 hours for later use;

[0043] (2) Synthesis of Au rods: After 3 hours, add 0.6ml 0.01M HAuCl to 40ml 0.1M CTAB solution in sequence 4 ,0.5ml0.01MAgNO 3 and 0.38ml of 0.1M ascorbic acid solution, stirred gently for 1 minute, after the solution became colorless, added 0.1ml of the above-mentioned synthetic seed solution, stirred gently for 2 minutes, and stood overnight. In this example, by adding AgNO 3 The amount of Au rods can be adjusted to adjust the aspect ratio, and the longitudinal absorption peak of the final synth...

Embodiment 2

[0049] This embodiment provides an application of a micro-fluorescent molecular fluorescence enhancement device, specifically: putting the fluorescence enhancement device prepared in Example 1 into a pre-prepared concentration of 10 -6 Fluorescent molecules of M (crystal violet, chemical structures such as figure 2 As shown; crystal violet is a molecule that is cheap and has high application value, such as being able to combine with protein molecules or DNA for biological imaging or detection, etc.) solution; take it out after 6 hours to test the photoluminescence performance; the photoluminescence performance adopts The test is excited by a 514nm laser. The fluorescence excitation wavelength of crystal violet molecules is around 580-590nm, and the emission wavelength is in the region of 630-640nm, but the luminous efficiency of this fluorescent molecule is relatively low, only 0.019; the test results show that with the increase of PMMA organic layer, the substrate Fluoresce...

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Abstract

The invention relates to a micro device for enhancing fluorescence of fluorescent molecules. The micro device comprises a base, multiple precious metal core-shell nanoparticles assembled on any surface of the base as well as a polymeric coating which is formed on the surface of the base and covers the precious metal core-shell nanoparticles, wherein the polymeric coating is 40-90 nm thick, and each precious metal core-shell nanoparticle is 20-50 nm thick. According to the micro device for enhancing the fluorescence of the fluorescent molecules, on one hand, the precious metal core-shell nanoparticles rich in free electrons are laid on any surface of the base, and the polymeric coating which covers the precious metal core-shell nanoparticles is formed on the surface of the base; on the other hand, the thickness of the precious metal core-shell nanoparticles and the polymeric coating can be accurately controlled, the fluorescence of the fluorescent molecules can be enhanced, and the reliability and the repeatability are better.

Description

technical field [0001] The invention belongs to the field of micro devices, and relates to a light enhancement device, in particular to a micro fluorescent molecular fluorescence enhancement device. Background technique [0002] Noble metal nanoparticles, especially gold and silver nanoparticles, have a unique surface plasmon resonance (LSPR) property under external stimuli due to their rich free electrons. The unique LSPR properties of noble metal nanoparticles make them have great research prospects and application value in molecular detection (SERS) and fluorescence enhancement (MEF). [0003] Studies have shown that there are many molecules that can fluoresce in nature. Many fluorescent molecules can also be synthesized artificially in modern times, but due to the relatively low luminous efficiency of molecules and the existence of limitations such as short fluorescence lifetimes, the application of fluorescent molecules in modern industry and medicine has been greatly ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/01G01N21/64
Inventor 江林孙俊孙迎辉谭昌会
Owner SUZHOU UNIV
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