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New camellia oleifera high-yield cultivation molecular design method

A molecular design and technology of camellia oleifera forest, applied in the biological field, can solve the problems of self-abortion, low-yield forest, and self-abortion of camellia oleifera forest, and achieve easy promotion and expansion, increase fruit setting rate and fruiting rate, and simple operation Effect

Active Publication Date: 2015-11-25
贵州山茶产业综合开发有限公司
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Problems solved by technology

But so far, there is no technical standard for the construction of Camellia oleifera heterocross forests, so it is impossible to fundamentally increase the yield of Camellia oleifera and achieve high yield and high efficiency of Camellia oleifera
[0005] The prior art mainly adopts bud stock grafting seedlings to build a new camellia oleifera forest. If the adjacent plants of the new camellia oleifera forest built by this method are the same genotype of self-incompatibility (SI), self-fertility is more likely to occur, thereby forming a low-yield plant. Forest
At present, there is no method to fundamentally solve the problem of how to avoid self-abortion in Camellia oleifera forest

Method used

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  • New camellia oleifera high-yield cultivation molecular design method
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  • New camellia oleifera high-yield cultivation molecular design method

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Effect test

Embodiment 1

[0026] 1. Select the Nei's genetic distance range of the best Camellia oleifera pollination combination

[0027] (1) Using 49 excellent clones of camellia oleifera as materials, the DNA was extracted respectively by using the improved CTAB method, and the steps were as follows:

[0028] 1) Put 10 grams of Camellia oleifera into a mortar, pour an appropriate amount of liquid nitrogen into the grinder, transfer to a centrifuge tube pre-filled with 700 μL 2×CTAB, place in a 65°C water bath for 45-60 minutes, and centrifuge (4°C, 1000rpm, 10min) Obtain supernatant I;

[0029] 2) Take 600 μL of the supernatant from the centrifuge tube in step 1) in a new centrifuge tube, add a mixture of phenol, chloroform and isoamyl alcohol (volume ratio 25:24:1) with a total volume of 600 μL, shake well and centrifuge ( 4°C, 1000rpm, 10min) to obtain supernatant II;

[0030] 3) Take 550 μL of the supernatant II in a centrifuge tube, add 500 μL of 10×CTAB, shake well, place in a 65°C water bath...

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Abstract

The invention relates to a new camellia oleifera high-yield cultivation molecular design method which mainly comprises the following three main steps: (1) determining a Neigenetic distance range of optimal oiltea camellia pollination combination by virtue of an SSR molecular recognition method; (2) detecting a natural cultivation population and wild population mating system; and (3) determining a high-outcross pollination allocation pattern. The Neigenetic distance range of optimal oiltea camellia pollination combination, which is obtained by virtue of the SSR molecular recognition method provided by the invention, is 0.431-0.480, and the fruit-setting rate and the yield rate are respectively not less than 80% and 59%; and by virtue of combination with the high-outcross pollination allocation pattern, the fruit-setting rate and yield rate of oiltea camellia are greatly improved, and the problem of low oiltea camellia yield is solved.

Description

technical field [0001] The invention relates to designing a high-yield cultivation configuration pattern of camellia oleifera through molecular recognition, and belongs to the field of biotechnology. Background technique [0002] Camellia oleifera is known as the world's four major woody oil plants along with olive, oil palm and coconut, and has a cultivation history of more than 2,300 years in my country. Camellia oil extracted from Camellia oleifera seeds is a high-quality green health-care edible oil with high nutritional value. The Food and Agriculture Organization of the United Nations has listed it as a healthy high-grade edible oil that is mainly promoted. Camellia oleifera has high economic value and strong ecological function. It is one of the excellent native tree species with excellent economic and ecological benefits unique to our country, and occupies a very important position in the southern economic forest industry. By the end of 2014, the area of ​​camellia ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02C12Q1/68
Inventor 阮成江
Owner 贵州山茶产业综合开发有限公司
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