New application of berberine serving as agricultural fungicide

An agricultural fungicide and new-purpose technology, applied in the field of pesticide application, can solve the problems of less research on berberine and achieve the effect of enriching plant varieties

Active Publication Date: 2015-11-25
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, berberine is mainly used in the field of medicine, but there are few stu...

Method used

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  • New application of berberine serving as agricultural fungicide
  • New application of berberine serving as agricultural fungicide
  • New application of berberine serving as agricultural fungicide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Inhibitory Effect of Berberine on Phytopathogenic Fungi

[0021] With reference to Mu Liyi's (1991) mycelial growth rate method, the influence of test samples on the mycelial growth rate of different plant pathogenic fungi was tested.

[0022] (1) Activation of the test strain: Inoculate the test fungus in a 9cm PSA plate culture dish and cultivate it in a constant temperature incubator at 28°C. Punch holes on the edge of the colony with a bacteria puncher to make a cake with a diameter of 7 mm for later use.

[0023] (2) Preparation of poisonous plate medium: Weigh an appropriate amount of test sample, dissolve it completely with methanol, dilute it with sterilized water to make a sample solution, draw the sample solution and add it to a quantitative 100mL hot melt and cool it down. Put it into the PSA hot medium at about 50°C, mix it well, pour it into a 9cm-diameter petri dish to make a poisonous medium with a uniform thickness, and fully solidify it for...

Embodiment 2

[0031] Example 2: Inhibitory effect of berberine on bacteria

[0032] Refer to the agar punching diffusion method in the zone of inhibition method, the operation steps are as follows:

[0033] (1) Preparation of bacterial liquid: Streak the tested bacteria in NA medium for activation, and culture them in a constant temperature incubator at 28°C for 48 hours, then pick the cultured bacteria into NA liquid culture with a sterilizing loop, and place them in 28°C, 120r / m shaking incubator for 24h shaking culture, set aside.

[0034] (2) Preparation of bacteria-carrying medium: Melt 100mL of NA medium completely, wait for it to cool to about 45°C, draw 1mL of the cultured bacterial suspension and inject it into the medium, shake it well, and pour it evenly into the medium In a 9cm petri dish, about 25mL NA medium per dish, set aside after solidification.

[0035] (3) Preparation of sample solution: take the completely dissolved sample solution, dilute it with sterilized water t...

Embodiment 3

[0043] Example 3: Toxicity of berberine to rice bacterial streak

[0044] Refer to Du Lianxiang (2005) OD value method to determine the toxicity of the samples to the tested bacteria.

[0045] (1) Strain preparation: the strain activation method is the same as above, and the absorbance of the pathogenic bacteria is tested by an ultraviolet spectrophotometer, and the bacterial liquid is diluted to 1×109cfu / mL for later use.

[0046](2) Sample solution preparation: take the completely dissolved sample solution, dilute it with sterilized water to prepare the required concentration, add it into 50mL liquid NA bottles (the control group contains the same amount of sample dissolution solvent), and prepare different concentrations of Drug-containing liquid medium, spare.

[0047] (3) Pipette 0.5 mL of bacterial liquid into 50 mL of liquid NA culture to make a bacterial suspension with a bacterial content of 1×107 cfu / mL. After mixing evenly, incubate at 28°C and 130r / m for 24h, t...

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Abstract

The invention belongs to the technical field of pesticide application, and relates to new application of berberine, in particular to new application of berberine serving as an agricultural fungicide. The new application refers to preventing and controlling plant diseases caused by plant pathogenic bacteria. Studies show that berberine has good inhibiting effect on various plant pathogenic bacteria, and a theoretic foundation is laid for developing berberine into a new pesticide.

Description

technical field [0001] The invention belongs to the technical field of pesticide application, and relates to a new application of berberine, in particular to a new application of berberine as an agricultural fungicide. Background technique [0002] Berberine is also called berberine. Is a common isoquinoline alkaloid, molecular formula C 20 h 18 NO 4 , whose structure is as follows: [0003] [0004] Berberine exists in many plants of four families and ten genera including Berberidaceae. In 1826 M.-E. Chavalier and G. Peltin obtained for the first time from the bark of Xanthoxylonclava. Berberine is a quaternary ammonium alkaloid. Yellow needle-like crystals can be precipitated from ether; melting point is 145°C; soluble in water, insoluble in benzene, ether and chloroform. It is mainly used to treat intestinal infections such as gastroenteritis and bacillary dysentery, conjunctivitis, and suppurative otitis media. Recently, it has also been found that this produc...

Claims

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Application Information

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IPC IPC(8): A01N43/90A01P3/00A01P1/00
Inventor 袁高庆周荣金黎起秦林纬
Owner GUANGXI UNIV
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