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Application of triterpenoid in preventing or treating hyperlipidemia

A technology for hyperlipidemia and composition, which is applied in the direction of medical preparations containing active ingredients, applications, food science, etc., and can solve problems such as reports on the therapeutic effect of betulinic acid that have not been seen.

Inactive Publication Date: 2015-11-25
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] So far there is no report about the therapeutic effect of betulinic acid on hyperlipidemia

Method used

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  • Application of triterpenoid in preventing or treating hyperlipidemia
  • Application of triterpenoid in preventing or treating hyperlipidemia
  • Application of triterpenoid in preventing or treating hyperlipidemia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] In order to study the effect of betulinic acid on hyperlipidemia, normal liver cells LO 2 The cells were planted in a 6-well plate, and after 18 hours of normal growth, they were starved for 6 hours and administered. group, cells were collected 24 hours after administration. For the determination of triglyceride content, cells were lysed with lysate for 10 min, 20 uL was taken from each group, 180 uL of triglyceride determination kit reagent was added, incubated for 30 min, and the absorbance at 546 nm was detected by a microplate reader. For the determination of the cholesterol content, the cholesterol in the cells was extracted, and the cholesterol content was detected by a gas phase-mass spectrometer (GC-MS). Because the n-hexane solvent was volatile, cholestane was used as an internal reference. The chromatographic column is a hp-5 capillary column (15m×0.25mm, 0.25μm); the carrier gas is helium, and the flow rate is 9psi; the inlet temperature is 295°C; the detect...

Embodiment 2

[0031] Preparation of betulinic acid

[0032] Tablets of betulinic acid

[0033] Table betulinic acid 10mg, starch 88g, magnesium stearate 3g

[0034] Preparation process: take epi-betulinic acid and pass through a 100-mesh sieve, add starch and magnesium stearate, mix evenly, make granules, dry, and press into tablets.

[0035] Epi-Betulinic Acid Capsules

[0036] Table betulinic acid 10mg, starch 88g, magnesium stearate 3g

[0037] Preparation process: Take epibetulinic acid and pass through a 100-mesh sieve, add starch and magnesium stearate, mix evenly, make granules, dry, pack into capsules, and obtain.

Embodiment 3

[0039] Food preparations expressing betulinic acid

[0040] 5g dry yeast, 90ml warm water, a little water, 150g flour, 5mg betulinic acid, 10g vegetable oil, a little low sodium salt

[0041] Biscuit method: Sprinkle the yeast in warm water and stir to dissolve, add betulinic acid. Stir in the flour, then add the vegetable oil and knead into a smooth dough; form the dough into 0.2cm thick sheets. Press out the shape, puncture holes, sprinkle water on the surface, sprinkle a little low-sodium salt, and ferment at room temperature for 10 minutes; preheat the oven at 120 degrees, place it on the upper layer, and bake for about 10 minutes to obtain food containing epi-betulinic acid.

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PUM

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Abstract

The invention relates to an application of epibetulinic acid in preventing or treating hyperlipidemia. According to the invention, epibetulinic acid which is commercially available or is prepared by the existing method, or pharmaceutical salts, aquo-complexes or anhydrides of epibetulinic acid are adopted, the epibetulinic acid is prepared into medicines, health products or functional foods, and excipients or carriers common in the pharmacy or the food field, such as diluents, disintegrating agents, and lubricating agents, are adopted. In the application, the epibetulinic acid is administered to LO2 cells, and after 24h, the contents of triglyceride and cholesterol are detected; the result shows that the epibetulinic acid has obvious effects in reducing cholesterol and triglyceride, and obvious statistical differences and good dose-effect relationship are also obtained.

Description

technical field [0001] The invention relates to the field of natural medicines, and relates to the use of betulinic acid to prevent or treat hyperlipidemia. Background technique [0002] Hyperlipidemia refers to excessive blood lipid levels, a metabolic disease in which one or more lipids in plasma are higher than normal due to abnormal fat metabolism or operation. Mainly include elevated triglycerides and cholesterol Elevated biliary triglycerides. The increase of blood lipid level will make the incidence of cardiovascular disease and mortality increase with the increase of serum total cholesterol and LDL cholesterol level. In addition, elevated triglycerides and cholesterol are also closely related to atherosclerosis, non-alcoholic fat, and type II diabetes. Therefore, it is of great clinical significance to develop good drugs for the treatment of hyperlipidemia. The liver is the main organ of lipid metabolism, so normal liver cells LO2 and liver cancer cells HepG2 are ...

Claims

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Application Information

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IPC IPC(8): A61K31/56A61P3/06A21D2/14A21D2/36A21D13/08
Inventor 李萍马世平万晓梦谢治深吴蒙徐晓军
Owner CHINA PHARM UNIV
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