A kind of organic fertilizer with high content of humic acid and preparation method thereof
A technology of humic acid and organic fertilizer, applied in the preparation of organic fertilizer, organic fertilizer, biological organic part treatment, etc., can solve the problems of random discharge of filter mud, environmental pollution, etc., achieve environmental protection, high humic acid conversion rate , the effect of high humic acid content
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Embodiment 1
[0026] A kind of organic fertilizer with high content of humic acid prepared by utilizing sulfite process sugar factory filter mud, comprising the following steps:
[0027] Step A: Screening of fermentation strains, select Bacillus subtilis (No. ACCC11060), Bacillus megaterium (No. ACCC10010) and jelly-like Bacillus (also known as silicate bacteria) (Bacillus mucilaeinosus, No. ACCC11103 ) mixed as fermentation strains, these three strains were purchased from the National Culture Collection Center, and the mass ratio of the three was 1:1:1, that is, each accounted for one-third.
[0028] Step B: Use the screened fermentation strains for the production of koji, operate under sterile conditions, inoculate and cultivate the purified strains, and the obtained strains are first-class seeds; shaker amplification, the obtained first-class seeds Inoculate into a triangular flask, cultivate and amplify the number of bacteria, and cultivate for 3-5 days. The cultured strain is the secon...
Embodiment 2
[0035] On the basis of embodiment 1, the bacterium koji of this example is made up of Bacillus subtilis, gigantic bacillus and jelly-like bacillus, and the amount of bacterium koji is 3.0 kilograms of bacterium koji per ton of sulfite sugar factory filter mud, to obtain organic Fat B.
[0036] Contrast test adopts the general fungus koji on the market, and general bacterium koji consumption is 5.0 kilograms of bacterium koji per ton of sulphite sugar factory filter mud, obtains conventional organic fertilizer; Described general bacterium koji is made of yeast and Bacillus subtilis (Bacillus subtilis, No. ACCC11068) composition.
[0037] The organic fertilizer B was compared with the conventional organic fertilizer, and the results are shown in Table 2. From the comparison of the data in Table 2, it can be seen that the humic acid content of organic fertilizer B is 30.1%, and the conventional organic fertilizer is only 16.8%. Organic fertilizer B is 13.3% higher than conventio...
Embodiment 3
[0041] On the basis of Example 1, the amount of bacterium koji used in this example is 4.5 kilograms of bacterium koji for every ton of sulfitation sugar factory filter mud to obtain organic fertilizer C.
[0042] Contrast test adopts the general fungus koji on the market, and general bacterium koji consumption is 5.0 kilograms of bacterium koji per ton of sulphite sugar factory filter mud, obtains conventional organic fertilizer; Described general bacterium koji is made of yeast and Bacillus subtilis (Bacillus subtilis, No. ACCC11068) composition.
[0043]The organic fertilizer C was compared with the conventional organic fertilizer, and the results are shown in Table 3. From the comparison of the data in Table 3, it can be seen that the humic acid content of organic fertilizer C is 31.2%, and that of conventional organic fertilizer is only 16.8%. Organic fertilizer C is 14.4% higher than conventional organic fertilizer, and has a higher humic acid conversion rate .
[0044...
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