Method for extracting beet pectin by using protopectinase

A technology of sugar beet pectin and protopectinase, which is applied in the field of food processing and processing, can solve the problems of difficult industrial application, poor pectin quality, low extraction rate and the like, and achieves high efficiency, specificity, convenient operation and high molecular weight Effect

Inactive Publication Date: 2015-11-25
常州毅博生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, the physical methods for extracting pectin mainly include ultrasonic method, high pressure sterilization method, pressing method and subcritical water extraction method, etc. The physical method is relatively simple and efficient, but it consumes a lot of energy and is difficult to apply industrially.
Enzymatic pecti

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] a. Raw material pretreatment: Weigh 80 meshes and 10kg beet pulp, add water with 8 times the mass of beet pulp, soak and wash at 85°C for 10 minutes, remove soluble sugar and impurities, and passivate the enzymes in the beet pulp at the same time. Filter with gauze to get wet beet pulp;

[0023] b. Enzymolysis extraction: add 250L citric acid-sodium citrate buffer solution (pH=4.7) to the wet beet pulp, add 2.5L protopectinase crude enzyme solution (enzyme activity 210U / ml) from Paenibacillus polymyxa ), reacted for 3 hours at 45°C and 150rpm;

[0024] c. Filtration: The pectin extract is filtered through the plate frame and the filter element successively to obtain the pectin collagen solution; the plate frame filter has 20 layers of filter membranes, the filtration accuracy is 20 μm, and the filter element filter is PP folding-20-3 type filter element type Filter, the filtration accuracy is 0.1μm;

[0025] d. Concentration by ultrafiltration: the filtered pectin sol...

Embodiment 2

[0029] a. Raw material pretreatment: Weigh 80 meshes and 10kg beet pulp, add water with 10 times the mass of beet pulp, soak and wash at 85°C for 15 minutes, remove soluble sugar and impurities, and passivate the enzymes in the beet pulp at the same time. Filter with gauze to get wet beet pulp;

[0030] b. Enzymolysis extraction: add 200L citric acid-sodium citrate buffer solution (pH=6.0) to wet sugar beet pulp, add 2.0L protopectinase crude enzyme solution (enzyme activity 150U / ml) from Bacillus subtilis, React at 55°C and 150rpm for 4 hours;

[0031] c. Filtration: The pectin extract is filtered through the plate frame and the filter element successively to obtain the pectin collagen solution; the plate frame filter has 20 layers of filter membranes, the filtration accuracy is 20 μm, and the filter element filter is PP folding-20-3 type filter element type Filter, the filtration accuracy is 0.1μm;

[0032] d. Concentration by ultrafiltration: the filtered pectin solution ...

Embodiment 3

[0036] a. Raw material pretreatment: Weigh 10 kg beet pulp of 80 mesh, add water 5-10 times the mass of beet pulp, soak and wash at 80-90°C for 5-15 minutes, remove soluble sugar and impurities, and passivate the beet pulp itself at the same time enzyme. Filter with gauze to get wet beet pulp;

[0037] b. Enzymolysis extraction: add 150L citric acid-sodium citrate buffer solution (pH=6.5) to the wet sugar beet pulp, add 1.5L protopectinase crude enzyme solution (enzyme activity 250U / ml) from Penicillium, React at 60°C and 150rpm for 3 hours;

[0038] c. Filtration: The pectin extract is filtered through the plate frame and the filter element successively to obtain the pectin collagen solution; the plate frame filter has 20 layers of filter membranes, the filtration accuracy is 20 μm, and the filter element filter is PP folding-20-3 type filter element Type filter, the filtration accuracy is 0.1μm;

[0039] d. Concentration by ultrafiltration: the filtered pectin solution is...

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Abstract

The invention discloses a method for extracting beet pectin by using protopectinase. The method comprises the following steps: 1, weighing 10kg of 80 mesh beet pulp, adding water with the amount 5-10 times the mass of beet pulp, carrying out immersion washing at 80-90DEG C for 5-15min to remove soluble sugar and impurities and passivate enzymes in the beet pulp, and filtering by using gauze to obtain wet beet pulp; 2, adding 100-300L of a citric acid-sodium citrate buffer solution with the pH value of 4.0-6.0 to the wet beet pulp, adding 1-2.5L of a crude protopectinase solution, and reacting at 40-70DEG C under 150rpm for 2-4h; 3, sequentially respectively carrying out plate and frame filtration and filter core filtration on a pectin extraction liquid to obtain a pectin stock solution, wherein a plate and frame filter has 20 layers of a filter membrane, the filtration precision of the plate and frame filter is 20[mu]m, a filter core filter is a PP folding-20-3 type filter core filter, and the precision of the filter core filter is 0.1[mu]m; 4, carrying out concentrating filtration on the filtered pectin stock solution through using a 8-tube tubular ultrafilter membrane to thoroughly remove impurities and concentrate the pectin stock solution to 3-5%; and 5, drying the concentrated and impurity-removed pectin stock solution through using an experimental high-speed centrifugal spray drier.

Description

Technical field: [0001] The invention relates to food processing and treatment, in particular to a method for extracting sugar beet pectin by protopectinase. Background technique: [0002] It is well known that pectin substances are high molecular weight, negatively charged acidic polysaccharide molecules. The main chain of the pectin molecule is connected by D-galacturonic acid with α-1,4-glucosidic bonds, which is the so-called "smooth region". The carboxyl groups of galacturonic acid on the main chain have different degrees of methyl groups ylated, while unmethylated carboxyl groups are associated with K 1+ 、Na 1+ and C 2+ The side chain is composed of polyrhamnogalacturonic acid, and neutral sugars such as polyarabinose, polygalactose and polyarabinogalactose are phased with the rhamnose in the side chain through α-1,4-glucosidic bonds connect. [0003] Pectin substances widely exist in the roots, stems, leaves and fruits of higher plants, and the pectin content is ...

Claims

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Application Information

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IPC IPC(8): C08B37/06
Inventor 陈佳李燕
Owner 常州毅博生物科技有限公司
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