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Method for determining content of methyl mercury in fish meat

A technology of methylmercury and fish meat, which is applied in the field of determination of methylmercury content in fish meat, can solve the problems of high instrument cost, difficulty in routine detection of methylmercury, and insufficient stability of derivative products, and achieve high extraction efficiency and small reagent consumption , high-precision effects

Inactive Publication Date: 2015-11-25
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method described in this invention has the advantages of accurate analysis method, high sensitivity, and little interference, but the method’s instrument cost is too high, and it is difficult to realize routine detection of methylmercury. At the same time, the derivative product after sample derivation has the disadvantage of not being stable enough

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for measuring methylmercury content in fish, comprising the following steps:

[0021] (1) Microwave extraction of methylmercury

[0022] Peel and remove the thorns from the commercially available tuna flesh, freeze in the refrigerator for 10 hours, dry in a freeze dryer for 24 hours, grind the dried fish flesh into powder, weigh 0.2g and place it in a microwave extraction tube, add 5mL of dichloro Methane and 0.5 mL of hydrochloric acid with a concentration of 6 mol / L were mixed evenly, and placed in a microwave extractor for extraction for 8 min at a temperature of 50°C. After the extraction is complete, take out the extraction tube, add 2mL of 2-mercaptoethanol solution, shake and back-extract methylmercury for 15min, after completion, put the extract in a centrifuge and centrifuge at 1000r / min for 3min, take out the water phase, and wash it with 6mol / L hydrochloric acid Dilute to 10mL, shake well and set aside.

[0023] The mass fraction of each component ...

Embodiment 2

[0031] A method for measuring methylmercury content in fish, comprising the following steps:

[0032] (1) Microwave extraction of methylmercury

[0033] Peel and remove the thorns from the commercially available tuna meat, freeze in the refrigerator for 10 hours, dry in a freeze dryer for 24 hours, grind the dried fish meat into powder, weigh 0.3g and put it in a microwave extraction tube, add 10mL of dichloro Methane and 1 mL of hydrochloric acid with a concentration of 6 mol / L were mixed evenly, and placed in a microwave extractor for extraction for 10 min at a temperature of 70°C. After the extraction is complete, take out the extraction tube, add 4mL of 2-mercaptoethanol solution, shake and back-extract methylmercury for 30min, after completion, put the extract in a centrifuge and centrifuge at 2000r / min for 5min, take out the water phase, and wash it with 6mol / L hydrochloric acid Dilute to 10mL, shake well and set aside.

[0034] The mass fraction of each component of t...

Embodiment 3

[0038] A method for measuring methylmercury content in fish, comprising the following steps:

[0039]Peel and remove the thorns from the commercially available tuna flesh, freeze in the refrigerator for 10 hours, dry in a freeze dryer for 24 hours, grind the dried fish flesh into powder, weigh 0.25 g and place it in a microwave extraction tube, add 10 mL of Chloromethane and 0.5 mL of hydrochloric acid with a concentration of 6 mol / L were mixed evenly, and placed in a microwave extractor for extraction for 10 min at a temperature of 60°C. After the extraction is complete, take out the extraction tube, add 3mL of 2-mercaptoethanol solution, shake and back-extract methylmercury for 20min, after completion, put the extract in a centrifuge and centrifuge at 1500r / min for 5min, take out the water phase, and wash it with 6mol / L hydrochloric acid Dilute to 10mL, shake well and set aside.

[0040] The mass fraction of each component of the above-mentioned 2-mercaptoethanol solution i...

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Abstract

The invention discloses a method for determining the content of methyl mercury in fish meat. The method includes the steps: after a fish meat sample is pretreated, extracting methyl mercury in a microwave extraction apparatus by adopting a mixed solution of dichloromethane and hydrochloric acid, then carrying out reverse extraction of methyl mercury by a 2-mercaptoethanol solution, taking a supernatant through centrifugation, finally determining the absorbance by flame atomic absorption spectrometry, and thus obtaining the concentration of methyl mercury in the sample through a standard curve. With combination of the microwave assisted extraction and the flame atomic absorption spectrometry, the content of methyl mercury in the fish meat is determined, and the method has the advantages of being higher in accuracy, convenient to operate, and safe and simple.

Description

technical field [0001] The invention relates to the field of food safety detection, in particular to a method for measuring the content of methylmercury in fish. Background technique [0002] Methylmercury is an environmental pollutant with strong neurotoxicity. When industrial wastewater, mercury-containing pesticides, inorganic mercury salts in mercury-containing waste gas, and inorganic mercury in the natural environment enter the ocean, they are absorbed by methylcobalamin. The effect becomes the organic heavy metal methylmercury, which is enriched in fish and aquatic organisms. Due to lipophilicity, bioaccumulation and biomagnification, methylmercury is hundreds of times more toxic than other mercury compounds. After methylmercury enters the human body, it spreads throughout the organs and tissues of the whole body, mainly invading the central nervous system, and can cause language and memory impairment. It is precisely because of the great toxicity of methylmercury t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/72G01N21/33
Inventor 夏松养
Owner ZHEJIANG OCEAN UNIV
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