Unlock instant, AI-driven research and patent intelligence for your innovation.

Chamaecyparis pisifera primary tissue culture method

A technology of tissue culture and culture medium, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of less branch formation, less ear sources, and inability to reproduce rapidly and in large quantities, so as to reduce pollution rate and improve survival rate effect

Active Publication Date: 2015-12-02
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
View PDF4 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Savannah is generally propagated by seed sowing and cuttings, but seed propagation is prone to variation, and the ear sources of cuttings are few, so it cannot be reproduced rapidly and in large quantities. Therefore, the method of tissue culture is a new way of rapid propagation of Savannah cypress
[0003] At present, in the primary tissue culture process of Savannah cypress, the culture medium, hormone type and concentration are all important, but the selection in the prior art is not reasonable enough, resulting in too few branches formed, prone to glassy flowers, browning and other phenomena

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Chamaecyparis pisifera primary tissue culture method
  • Chamaecyparis pisifera primary tissue culture method
  • Chamaecyparis pisifera primary tissue culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Preparation of the medium: MS medium was selected as the basic medium, sucrose and agar were added to the concentration of 30g / L and 7g / L, and 6-benzylamino adenine (6-BA) was added to the concentration of 0.05mg / L, adjust the pH to 5.8~6.0, autoclave at 121℃ for 25min, it is obtained;

[0022] (2) Treatment of explants: Choose explants without buds, soak them with detergent, rinse with pure water for 2 hours, then treat them with 75% alcohol for 30 seconds, and then soak them with 0.1% mercury. 5min, finally rinse with sterile water for 5 times, and absorb the residual water with sterile filter paper;

[0023] (3) Inoculation culture: Cut off the old wound of the explant obtained in step (2), insert it into the culture medium obtained in step (1), and place it in a culture room for culture. The culture conditions are: light 16h / d, light intensity of 2000lx, temperature Control at (25±2)℃.

Embodiment 2

[0025] (1) Preparation of medium: choose B5 medium as the basic medium, add sucrose and agar, the concentrations are 30g / L and 7g / L, and then add 6-benzylamino adenine, the concentration is 1mg / L, adjust The pH value is 5.8~6.0, and it is sterilized under high pressure at 121℃ for 25min, and it is obtained

[0026] (2) Treatment of explants: Choose explants without buds, soak them with detergent, rinse with pure water for 2 hours, then treat them with 75% alcohol for 30 seconds, and then soak them with 0.1% mercury. 8min, finally rinse with sterile water for 5 times, and absorb the residual water with sterile filter paper;

[0027] (3) Inoculation culture: Cut off the old wound of the explant obtained in step (2), insert it into the culture medium obtained in step (1), and place it in a culture room for culture. The culture conditions are: light 16h / d, light intensity of 2000lx, temperature Control at (25±2)℃.

Embodiment 3

[0029] (1) Preparation of the medium: Choose 1 / 2MS medium as the basic medium, add sucrose and agar, the concentrations are 30g / L and 7g / L, and then add naphthalene acetic acid, the concentration is 0.05mg / L, adjust the pH The value is 5.8~6.0, sterilized under high pressure at 121℃ for 25min, it is obtained;

[0030] (2) Treatment of explants: Choose explants without buds, soak them with detergent, rinse with pure water for 2 hours, then treat them with 75% alcohol for 30 seconds, and then soak them with 0.1% mercury. 6min, finally rinse with sterile water 5 times, and absorb the residual water with sterile filter paper;

[0031] (3) Inoculation culture: Cut off the old wound of the explant obtained in step (2), insert it into the culture medium obtained in step (1), and place it in a culture room for culture. The culture conditions are: light 16h / d, light intensity of 2000lx, temperature Control at (25±2)℃.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a chamaecyparis pisifera primary tissue culture method which comprises the following steps: (1) preparation of a culture medium: selecting a basal culture medium, adding sucrose and agar, then adding 6-benzylamino adenine (6-BA) or naphthylacetic acid, adjusting the pH value, and sterilizing to obtain the culture medium; (2) processing of explants: selecting explants without buds, cleaning and sterilizing; and (3) inoculated culture: cutting off old wounds of the explants obtained in the step (2), inserting into the culture medium obtained in the step (1), and putting in a culture room for culturing. Compared with the prior art, the method disclosed by the invention has the advantages of tissue culture and propagation in the prior art and is capable of obviously reducing the pollution rate of chamaecyparis pisifera, increasing the survival rate of chamaecyparis pisifera and enabling chamaecyparis pisifera to successfully form more branches and not no generate the phenomena of vitrification, browning and the like by being combined with other treatment methods through the specific culture medium as well as hormone types and concentration.

Description

Technical field [0001] The invention relates to a first generation tissue culture method of Savannah, belonging to the technical field of seedling propagation. Background technique [0002] Savannah is a newly introduced color-leaf plant that is yellow in all seasons. Because of its streamlined branches and bright colors, it has been widely used in gardens in Japan, but it has not been seen in landscaping in my country. Savannah generally uses seed sowing and cutting methods to propagate, but seed propagation is prone to variation, cutting propagation has fewer ear sources, and can't multiply quickly, so tissue culture is a new way for Savannah to rapidly propagate. [0003] At present, in the initial tissue culture process of Savannah, the medium, hormone type and concentration are all important, but the selection in the prior art is not reasonable enough, resulting in too few branches, glass blossoms and browning. And other phenomena. Summary of the invention [0004] Purpose of...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 周余华周琴蒋涛王磊于健王红梅
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY