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A kind of first-generation tissue culture method of Savannah cypress

A tissue culture and culture medium technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of less branch formation, less ear source, and inability to multiply rapidly and in large quantities, so as to reduce the pollution rate and improve the survival rate. rate effect

Active Publication Date: 2017-06-13
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Savannah is generally propagated by seed sowing and cuttings, but seed propagation is prone to variation, and the ear sources of cuttings are few, so it cannot be reproduced rapidly and in large quantities. Therefore, the method of tissue culture is a new way of rapid propagation of Savannah cypress
[0003] At present, in the primary tissue culture process of Savannah cypress, the culture medium, hormone type and concentration are all important, but the selection in the prior art is not reasonable enough, resulting in too few branches formed, prone to glassy flowers, browning and other phenomena

Method used

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  • A kind of first-generation tissue culture method of Savannah cypress
  • A kind of first-generation tissue culture method of Savannah cypress
  • A kind of first-generation tissue culture method of Savannah cypress

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) The preparation of medium: select MS medium as basic medium, add sucrose and agar, concentration is respectively 30g / L and 7g / L, then add 6-benzylaminoadenine (6-BA), its concentration is 0.05mg / L, adjust the pH value to 5.8-6.0, and sterilize under high pressure at 121°C for 25 minutes to get it;

[0022] (2) Explant treatment: select explants without buds, first soak them with washing powder aqueous solution, then rinse with pure water for 2 hours, then treat them with 75% alcohol for 30 seconds, and then use 0.1% mercuric chloride Soak for 5 minutes, and finally rinse 5 times with sterile water, and absorb the residual water with sterile filter paper;

[0023] (3) Inoculation and cultivation: the old wound of the explant obtained in step (2) is cut off, inserted into the obtained medium of step (1), and placed in a cultivation room for cultivation, the cultivation conditions are: light 16h / d, light intensity is 2000lx, temperature Control at (25±2)°C.

Embodiment 2

[0025] (1) The preparation of medium: select B5 medium as base medium, add sucrose and agar, concentration is 30g / L and 7g / L respectively, then add 6-benzylaminoadenine, its concentration is 1mg / L, regulates The pH value is 5.8-6.0, and it is sterilized under high pressure at 121°C for 25 minutes, and it is ready;

[0026] (2) Explant treatment: select explants without buds, first soak them with washing powder aqueous solution, then rinse with pure water for 2 hours, then treat them with 75% alcohol for 30 seconds, and then use 0.1% mercuric chloride Soak for 8 minutes, and finally rinse 5 times with sterile water, and absorb the residual water with sterile filter paper;

[0027] (3) Inoculation and cultivation: the old wound of the explant obtained in step (2) is cut off, inserted into the obtained medium of step (1), and placed in a cultivation room for cultivation, the cultivation conditions are: light 16h / d, light intensity is 2000lx, temperature Control at (25±2)°C.

Embodiment 3

[0029] (1) Preparation of medium: select 1 / 2MS medium as the basic medium, add sucrose and agar, the concentration is 30g / L and 7g / L respectively, then add naphthaleneacetic acid, its concentration is 0.05mg / L, adjust the pH The value is 5.8-6.0, and it is sterilized under high pressure at 121°C for 25 minutes to get it;

[0030] (2) Explant treatment: select explants without buds, first soak them with washing powder aqueous solution, then rinse with pure water for 2 hours, then treat them with 75% alcohol for 30 seconds, and then use 0.1% mercuric chloride Soak for 6 minutes, and finally rinse 5 times with sterile water, and absorb the residual water with sterile filter paper;

[0031] (3) Inoculation and cultivation: the old wound of the explant obtained in step (2) is cut off, inserted into the obtained medium of step (1), and placed in a cultivation room for cultivation, the cultivation conditions are: light 16h / d, light intensity is 2000lx, temperature Control at (25±2)°...

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Abstract

The invention discloses a chamaecyparis pisifera primary tissue culture method which comprises the following steps: (1) preparation of a culture medium: selecting a basal culture medium, adding sucrose and agar, then adding 6-benzylamino adenine (6-BA) or naphthylacetic acid, adjusting the pH value, and sterilizing to obtain the culture medium; (2) processing of explants: selecting explants without buds, cleaning and sterilizing; and (3) inoculated culture: cutting off old wounds of the explants obtained in the step (2), inserting into the culture medium obtained in the step (1), and putting in a culture room for culturing. Compared with the prior art, the method disclosed by the invention has the advantages of tissue culture and propagation in the prior art and is capable of obviously reducing the pollution rate of chamaecyparis pisifera, increasing the survival rate of chamaecyparis pisifera and enabling chamaecyparis pisifera to successfully form more branches and not no generate the phenomena of vitrification, browning and the like by being combined with other treatment methods through the specific culture medium as well as hormone types and concentration.

Description

technical field [0001] The invention relates to a first-generation tissue culture method of Savannah cypress, which belongs to the technical field of nursery stock propagation. Background technique [0002] Savannah cypress is a newly introduced plant with colorful leaves that is yellow in all seasons. Because of its streamlined branches and bright colors, it has been widely used in gardens in Japan, but it has not been seen in landscaping in my country. Savannah is generally propagated by seed sowing and cuttings, but seed propagation is prone to variation, and the ear sources of cuttings are less, so it cannot be reproduced quickly and in large quantities. Therefore, the method of tissue culture is a new way of rapid propagation of Savannah cypress. [0003] At present, in the primary tissue culture process of Savannah cypress, the culture medium, hormone type and concentration are all important, but the selection in the prior art is not reasonable enough, resulting in too...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 周余华周琴蒋涛王磊于健王红梅
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY