Method for efficiently preparing pneumocandin B0

Abstract

The invention discloses a method for efficiently preparing pneumocandin B0 by separating pneumocandin B0 from fermentation broth. As a membrane separation technique is adopted to prepare pneumocandin B0, the method is relatively applicable to industrial continuous operation. Due to the adoption of an acid precipitation method, a great deal of pigment can be reduced, and B0 loss can be reduced; through silicagel column purification, an isomeride C0 can be effectively removed, and the extraction purity can be up to 99%. The whole process is simple, convenient and feasible, low in power consumption, small in reagent toxicity, and beneficial for industrial application.

Description

technical field [0001] The invention relates to the field of medicinal chemistry, in particular to a method for efficiently preparing pneumocidine B0. Background technique [0002] Neomercontin B 0 (pneumocandinB 0 )By Glarealozoyensis produced secondary metabolites, Glarealozoyensis In addition to producing pneumocantine B during fermentation 0 In addition to the structural analogue A 0 and isomer C 0 . As the precursor compound of the antifungal drug caspofungin, the preparation of high-purity pneumocontin B 0 It is essential for the refined purification of caspofungin. [0003] Chinese invention patent 200910133118.0 discloses the preparation of pneumocandine B 0 The method, the main steps are: a) centrifugal nemocontin B 0 Fermentation broth, take the mycelia, and extract neomocontin B with methanol 0 ; b) Evaporate the methanol extract to dryness, and then use n-butanol to extract Neomocontin B 0 ; c) the n-butanol extract is evaporated to dryness, and then ...

AI Technical Summary

Problems solved by technology

Although this method uses a dynamic axial compression column to replace the traditional silica gel column, the solid-liquid separation in the process still uses traditional centrifugal and rotary evaporation. The separation process has low efficiency, high energy consumption, and large losses.

[0006] In the prior art, centrifugation or evaporation is mostly used for separation and concentration in the preparation process, and adsorption resin or activated carbon is used for impurity removal and purification. The process efficiency is low, the recovery rate is low, and the energy consumption is high.

Moreover, more toxic substances are used in the process, which makes it difficult to control the environment