Mycoplasma pneumoniae antigen detection kit and detection method thereof

Abstract

The invention provides a mycoplasma pneumoniae antigen detection kit and a detection method. The mycoplasma pneumoniae antigen detection kit comprises a reagent plate, sample extraction liquid and sample pipes. The reagent plate comprises a clamping plate and a reagent strip. The outer shell of the reagent plate is the clamping plate, and the reagent strip is located in the clamping plate. The upper surface of the clamping plate is provided with a sample adding hole and a detection window. The reagent strip comprises a PVC plate, a chromatography membrane, a sample pad, a combined pad and a water absorbing material. The sample adding hole in the clamping plate is located above the sample pad. The combined pad contains a mycoplasma pneumoniae monoclonal antibody labeled with nano microspheres. The chromatography membrane comprises a detection line T and a quality control line C which are located in the detection window in the clamping plate. The line T contains the mycoplasma pneumoniae monoclonal antibody, and the line C contains a goat anti-mouse igG polyclonal antibody. According to the kit, the window detection period can be obviously shortened, treatment can be carried out in time, detection time is short, operation is easy and convenient, the detection specificity is high, and the sensitivity is high.

Description

technical field [0001] The invention belongs to the field of medical detection, and relates to a mycoplasma pneumoniae antigen detection kit and a detection method thereof. Background technique [0002] Mycoplasma pneumoniae( M. Pneumonia ) is the causative agent of mycoplasma pneumonia in humans. The pathological changes of mycoplasma pneumonia are mainly interstitial pneumonia, sometimes complicated by bronchial pneumonia, which is called primary atypical pneumonia. It is mainly transmitted by droplets, with an incubation period of 2 to 3 weeks, and the highest incidence rate is among adolescents. [0003] The clinical manifestations and chest X-ray examination of mycoplasma pneumonia are not characteristic, and the diagnosis cannot be made based on clinical manifestations and chest X-ray examination alone. Definitive diagnosis requires detection of the pathogen. Existing Mycoplasma pneumoniae ( M. Pneumonia ) detection methods mainly include: pathogen isolation cultu...

AI Technical Summary

Problems solved by technology

[0004] Chinese patent application document CN104673718A discloses "a human mycoplasma pneumoniae culture medium and its diagnostic kit", which uses the method of pathogen isolation and culture to detect pathogens, but the detection window period of this method is long (more than 7 days for virus infection), It is easy to cause false positives or false negatives; the detection process is complicated and takes a long time; the specificity is low, the naked eye judges, and cannot be quantified

[0007] In addition, the microplate reader enzyme immunoassay (EIA) also has the defect of high requirements for detection equipment, microplate reader and detection technology.

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