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A feed additive, Vibrio hemolyticus technology, applied in microcapsules, animal feed, capsule transportation, etc., can solve the problems of lack and difficulty in control, and achieve the effects of enhancing resistance, improving economic benefits, and improving the survival rate of breeding.
Inactive Publication Date: 2015-12-09
YANGZHOU UNIV
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[0003] At present, it is difficult to control the occurrence and prevalence of diseases caused by Vibrio parahaemolyticus in the culture and production of Litopenaeus vannamei, and there is a lack of an anti-pathogenic Vibrio parahaemolyticus feed additive on the market that is convenient to use and suitable for the culture and production of Litopenaeus vannamei
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Embodiment 1
[0029] Example 1. A microcapsule feed additive for anti-L. vannamei pathogen Vibrio parahaemolyticus, the additive is made of the following three raw materials:
[0030] (1) 80% freeze-dried powder of yolk antibody against Vibrio parahaemolyticus, the pathogen of Litopenaeus vannamei;
[0031] (2) Astragalus powder 12%;
[0032] (3) β-cyclodextrin 8%;
[0033] The preparation steps of the lyophilized powder of the yolk antibody against the pathogen Vibrio parahaemolyticus of Litopenaeus vannamei are as follows:
[0034] ① The pathogen Vibrio parahaemolyticus that will cause Litopenaeus vannamei disease ( Vibrioparahaemolyticus ) The VP1 strain was inoculated on a common nutrient broth medium, cultured in a shaker at 28-30°C for 24 hours, and inactivated at 28°C for 48 hours by adding formaldehyde with a final concentration of 0.3% by volume, and centrifuged to obtain inactivated bacteria;
[0035] ② Take Astragalus membranaceus soaked in distilled water for 30-60min, decoct, filter, and ...
Embodiment 2
[0038] Example 2. A microcapsule feed additive for anti-L. vannamei pathogen Vibrio parahaemolyticus, which is made of the following three raw materials:
[0039] (1) 85% freeze-dried powder of yolk antibody against Vibrio parahaemolyticus, the pathogen of Litopenaeus vannamei;
[0040] (2) Astragalus powder 10%%;
[0041] (3) β-cyclodextrin 5%;
[0042] The preparation steps of the lyophilized powder of the yolk antibody against the pathogen of Litopenaeus vannamei pathogen Vibrio parahaemolyticus are the same as in Example 1.
Embodiment 3
[0043] Example 3, a microcapsule feed additive against the pathogen Vibrio parahaemolyticus of Litopenaeus vannamei, the additive is made of the following three raw materials:
[0044] (1) 82.5% of yolk antibody freeze-dried powder against Vibrio parahaemolyticus, the pathogen of Litopenaeus vannamei;
[0045] (2) Astragalus powder 11%;
[0046] (3) β-cyclodextrin 6.5%;
[0047] The preparation steps of the lyophilized powder of the yolk antibody against the pathogen of Litopenaeus vannamei pathogen Vibrio parahaemolyticus are the same as in Example 1.
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Abstract
The invention provides a microcapsule feed additive resisting to litopenaeus vannamei pathogenic vice hemolysis vibrio. The additive is composed of litopenaeus vannamei pathogenic vice hemolysis vibrio egg yolk antibody freeze-dried powder, astragalus powder and beta-cyclodextrin. The litopenaeus vannamei pathogenic vice hemolysis vibrio egg yolk antibody freeze-dried powder is mainly used for lowering or eliminating the possibility that the litopenaeus vannamei is infected by the vice hemolysis vibrio. The astragalus powder is used for improving the immunity of the litopenaeus vannamei. The beta-cyclodextrin is mainly used for making the egg yolk antibody freeze-dried powder play the embedding slow-release effect. According to the additive, spray drying is conducted on the obtained litopenaeus vannamei pathogenic vice hemolysis vibrio egg yolk antibody freeze-dried powder with the valence of the antibody reaching 211, the astragalus powder and the beta-cyclodextrin, microcapsule dry powder is obtained, the microcapsule feed additive and litopenaeus vannamei feed sold on the market are used for feeding litopenaeus vannamei together according to a certain proportion, the diseases caused by the pathogenic vice hemolysis vibrio can be prevented and treated in the breeding period of the litopenaeus vannamei, the breeding survival rate of the litopenaeus vannamei is raised, and the economic benefits of litopenaeus vannamei breeding and production are remarkably increased; the problem that at present the red body disease is caused by the vice hemolysis vibrio easily in the litopenaeus vannamei breeding and production processes is solved.
Description
Technical field [0001] The invention relates to a feed additive for aquaculture, in particular to a microcapsule feed additive for preventing Litopenaeus vannamei red body disease. Background technique [0002] With the expansion of the scale of Litopenaeus vannamei breeding, the ecological environment of breeding has deteriorated, and the frequency of disease has increased sharply. The pathogen Vibrio parahaemolyticus ( Vibrioparahaemolyticus ) The red body disease caused is widespread in all shrimp farming areas across the country, and it spreads quickly, which can cause the devastating death of Litopenaeus vannamei in mariculture. It has become the most common in Litopenaeus vannamei cultivation and causes the most serious economic losses. One of the diseases. Therefore, the efficient prevention and control of Vibrio parahaemolyticus, the pathogen of Litopenaeus vannamei, to minimize its damage will significantly improve the economic benefits of Litopenaeus vannamei breeding a...
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