Preparation method and application of transferrin modified hollow mesoporous copper sulfide/artesunate nanoparticles

A technology of mesoporous copper sulfide and artesunate, applied in the field of medicine, can solve problems such as no public reports, achieve huge economic and social benefits, improve therapeutic effect, and simple synthesis process

Active Publication Date: 2015-12-09
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far there is no public report of this technology

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  • Preparation method and application of transferrin modified hollow mesoporous copper sulfide/artesunate nanoparticles

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] In concrete implementation, the present invention can be realized by the following steps:

[0017] (1), 0.85g of CuSO 4 ·5H 2 Dissolve O in 200ml water, stir and add 5.0g polyvinylpyrrolidone, react at room temperature for 10-20min, add 1.5g sodium hydroxide, stir evenly, add 15ml hydrazine hydrate dropwise with a syringe, stir for 10-15min, then add 1.38g ammonium sulfate, Stir for 1 hour, centrifuge and wash with ultrapure water until neutral, and freeze-dry for 48 hours to obtain hollow mesoporous copper sulfide nanoparticles;

[0018] (2) Ultrasonically disperse 30 mg of hollow mesoporous copper sulfide nanoparticles in 28 ml of phosphate buffer solution with pH 7 to 9, add 120 mg of artesunate under stirring, and stir for 24 hours to obtain copper sulfide loaded with drug artesunate Nanoparticles (AS / CuS);

[0019] (3) Add 15 mg of transferrin into 10 ml of phosphate buffer solution with pH 7 to 9, stir to dissolve, then add to the above-mentioned copper sulfide...

Embodiment 2

[0021] The present invention can also be realized by the following steps in concrete implementation:

[0022] (1), 0.8g of CuSO 4 ·5H 2 Dissolve O in 180ml water, stir and add 4.5g polyvinylpyrrolidone, react at room temperature for 10-20min, add 1g sodium hydroxide, stir well, add 10ml hydrazine hydrate dropwise with a syringe, stir for 10-15min, then add 1.2g ammonium sulfate, stir 1h, wash with ultrapure water to neutrality, and freeze-dry for 46-50h to obtain hollow mesoporous copper sulfide nanoparticles;

[0023] (2) Ultrasonically disperse 25 mg of hollow mesoporous copper sulfide nanoparticles in 25 ml of phosphate buffer solution with pH 7 to 9, add 110 mg of artesunate under stirring, and continue stirring for 24 hours to obtain the vulcanization of drug-loaded artesunate. Copper nanoparticles (AS / CuS);

[0024] (3) Add 10 mg of transferrin into 8 ml of phosphate buffer solution with pH 7 to 9, stir to dissolve, then add to the above-mentioned copper sulfide nanop...

Embodiment 3

[0026] In specific implementation, the present invention can also be realized by the following steps:

[0027] (1), 0.9g of CuSO 4 ·5H 2 Dissolve O in 220ml water, stir and add 5.5g polyvinylpyrrolidone, react at room temperature for 10-20min, add 2g sodium hydroxide, stir well, add 20ml hydrazine hydrate dropwise with a syringe, stir for 10-15min, then add 1.4g ammonium sulfate, stir 1h, centrifuged with ultrapure water until neutral, and freeze-dried for 46-50h to obtain hollow mesoporous copper sulfide nanoparticles;

[0028] (2) Ultrasonically disperse 35 mg of hollow mesoporous copper sulfide nanoparticles in 30 ml of phosphate buffer solution at pH 7 to 9, add 130 mg of artesunate under stirring, and continue stirring for 24 hours to obtain the vulcanization of drug-loaded artesunate Copper nanoparticles (AS / CuS);

[0029] (3) Add 20 mg of transferrin into 12 ml of phosphate buffer solution with pH 7-9, stir to dissolve, then add to the above-mentioned copper sulfide ...

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Abstract

The invention relates to a preparation method and an application of transferrin modified hollow mesoporous copper sulfide / artesunate nanoparticles and effectively and simultaneously achieves targeting, photothermal therapy, photodynamic therapy, photoacoustic tomography, drug therapy and DMR (diffusion molecular retention) effect to realize integration of diagnosis and treatment. The method includes: synthesizing hollow mesoporous copper sulfide nanoparticles, loading artesunate in copper sulfide in a hollow mesoporous structure, and modifying with transferrin through electrostatic adherence to obtain the transferrin modified hollow mesoporous copper sulfide / artesunate nanoparticles. The synthesis process is simple, anti-cancer drugs are sent to cancerous parts by means of peri-cancerous injection, and accordingly treatment effects can be further improved, and toxic and side effects on normal tissues and cells are reduced. A function of infrared photothermal therapy is realized while photodynamic therapy and photoacoustic tomography can be carried out, and accordingly integration of photothermal therapy, photodynamic therapy, chemotherapy, tumor diagnosis and comprehensive therapy is realized. The transferrin modified hollow mesoporous copper sulfide / artesunate nanoparticles realize a great innovation of drugs for tumor treatment.

Description

technical field [0001] The invention relates to medicine, in particular to a preparation method and application of transferrin-modified hollow mesoporous copper sulfide / artesunate nanoparticles. Background technique [0002] Photothermal therapy technology is an emerging minimally invasive technology. It uses photothermal conversion materials to convert the energy of near-infrared lasers into thermal energy to achieve local high temperature, thereby killing cancer cells, and basically has no effect on normal tissue cells. It has a good application prospect in tumor therapy. As a new photothermal conversion material, CuS nanoparticles have strong near-infrared absorption and high photothermal conversion efficiency, and CuS nanoparticles can be degraded by organisms without obvious biological toxicity. Tumor therapy has received extensive attention. Copper sulfide nanoparticles can perform photocatalytic effect under near-infrared light to generate active oxygen for photodyn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/48A61K47/42A61K31/357A61K41/00A61K49/00A61P35/00
Inventor 侯琳单晓宁张振中冯倩华郝丽莎袁玉洁王用超
Owner ZHENGZHOU UNIV
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