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Method for extracting, separating and purifying imperatorin and cnidium lactone from fructus cnidii

A technology for separation and purification of imperatorin, applied in the field of chemical industry, can solve the problems of difficult selection of solvent system, lack of theoretical guidance, cumbersome operation process, etc., and achieve the effect of improving extraction efficiency, high purity, and simple operation

Inactive Publication Date: 2015-12-09
LIAOCHENG UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

According to literature reports, the methods for separating and purifying Cnidium coumarin mainly include repeated silica gel column chromatography and high-speed countercurrent chromatography. Strong organic solvent; the choice of solvent system in high-speed countercurrent chromatography is difficult and lacks theoretical guidance

Method used

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  • Method for extracting, separating and purifying imperatorin and cnidium lactone from fructus cnidii
  • Method for extracting, separating and purifying imperatorin and cnidium lactone from fructus cnidii
  • Method for extracting, separating and purifying imperatorin and cnidium lactone from fructus cnidii

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Embodiment

[0033] Example: The method for extracting, separating and purifying imperatorin and osthole from Fructus Cnidii, the steps are:

[0034] (1) Extraction of Cnidium coumarin: Grind the medicinal material of Cnidium, heat and reflux extraction with 8 times the amount of 85% ethanol for 4 times, each time for 2 hours, filter, combine the ethanol extracts, and concentrate under reduced pressure to obtain the crude extract .

[0035] (2) Separation and analysis of cnidium coumarin: use analytical high performance liquid chromatography to separate and analyze the composition of the crude extract, and the chromatographic column is C 18 Column (250mm×4.6mmI.D., 5μm), the mobile phase is methanol-water (67:33, V / V); the flow rate is 1.0mL / min, the detection wavelength is 254nm, and the column temperature is room temperature.

[0036] (3) Separation and purification of cnidium coumarin: the components in the crude extract were separated and purified by semi-preparative high performanc...

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Abstract

The invention relates to a method for extracting, separating and purifying imperatorin and cnidium lactone from fructus cnidii. The method takes the medicinal material fructus cnidii as a material, and comprises the following steps: (1) extraction; (2) high performance liquid chromatography separation and analysis; (3) semipreparative high performance liquid chromatography separation and purification; (4) purity detection and structural identification of the compounds. The separation and purification are carried out by using a semipreparative high performance liquid chromatography, wherein a mobile phase is of methanol-water, two components with high purity are obtained, and the two components respectively are the imperatorin and cnidium lactone through identification. The technological process is green and environment-friendly, serious harm to the environment does not exist, and the comprehensive cost is low.

Description

technical field [0001] The invention belongs to the field of chemical industry, and in particular relates to a method for extracting, separating and purifying imperatorin and osthole from cnidium. Background technique [0002] Cnidium is a plant of the genus Cnidium in the family Umbelliferae Cnidium monnieri The dried ripe fruit of (L.) Cuss. has the effects of warming kidney and strengthening yang, expelling cold and expelling wind, drying dampness and killing insects. The chemical components in Fructus Cnidii are mainly coumarins and volatile oils. The reported coumarins include osthole, imperatorin, etc., among which osthole is the main active ingredient, and its content accounts for 60% of the total coumarins Left and right are the main indicators for the quality control of Fructus Cnidii and related preparations. Pharmacological studies have shown that Fructus Cnidii has anti-inflammatory, anti-mutagenic, antibacterial, anti-tumor, anti-arrhythmic, anti-osteoporosis,...

Claims

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Application Information

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IPC IPC(8): C07D493/04C07D311/16
CPCC07D493/04C07D311/16
Inventor 李爱峰亢静静柳仁民孙爱玲
Owner LIAOCHENG UNIV
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