Preparation method and applications of human odor binding protein sensor of nano-pore array

Abstract

The present invention discloses a preparation method and applications of a human odor binding protein sensor of a nano-pore array. According to the present invention, the nano-pore array prepared through a standard two-step anode oxidization method is used, the nano-pore array is treated with 2-carboxyethylphosphonic acid so as to immobilize human odor binding protein, and the human odor binding protein immobilizing nano-pore array is fixed in a designed reaction dish so as to obtain the sensor; the electrochemical impedance testing method and the equivalent circuit analysis method are used to immobilize the human odor binding protein onto the nano-pore array so as to be used for detecting disease-related fatty acid (docosahexaenoic acid) molecules and aldehyde molecules (benzaldehyde); and the constructed electrochemical sensor has characteristics of high detection sensitivity and low detection lower limit.

Description

technical field [0001] The present invention relates to a preparation technology of a sensor, in particular to a method for constructing a human odor-binding protein sensor using a nanopore array, and a human odor-binding protein sensor using a nanopore array to detect disease-related fatty acids (docohexa enoic acid) molecules and aldehyde-like molecules (benzaldehyde). Background technique [0002] Human odorant binding protein is a kind of lipocalin, which is a low-molecular-weight, soluble protein, and mainly exists in the lymph fluid outside the human olfactory nerve cells. Its function is to reversibly combine with target fat-soluble molecules, and transport such small hydrophobic molecules to the olfactory receptors on olfactory cells, thereby promoting the generation of smell. Humans can detect and distinguish hundreds of millions of different odor stimuli, among which human odorant binding protein plays a very important role. At this stage, sensors based on odoran...

AI Technical Summary

Problems solved by technology

At this stage, sensors based on odorant-binding proteins have attracted the attention of many people, and research on sensors such as quartz crystal microbalance, surface plasmon resonance, and electrochemical impedance has also been carried out, but the detection limit and sensitivity still cannot meet the requirements.

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