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Method for Determination of κ-Casein Content in Milk

A technology of casein and milk, applied in the direction of measuring devices, material analysis by electromagnetic means, instruments, etc., can solve problems that need to be improved, and achieve the effects of preventing adsorption, short centrifugation time, and high fat removal rate

Active Publication Date: 2017-11-10
INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, current methods for the determination of κ-casein in milk still need to be improved

Method used

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  • Method for Determination of κ-Casein Content in Milk
  • Method for Determination of κ-Casein Content in Milk
  • Method for Determination of κ-Casein Content in Milk

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] In this example, the kappa-casein content in raw milk was determined by the following steps:

[0078] (1) Raw milk pretreatment

[0079] Take 30ml of raw milk to be tested, put it in a 50ml centrifuge tube, and centrifuge at 6000r / min for 20 minutes to remove milk fat; then put it in a -40°C refrigerator for 20 minutes; take it out and return it to room temperature, and use 40mmol / L acetic acid aqueous solution to adjust the pH to 4.6, centrifuge at 6000r / min for 20 minutes, discard the supernatant, wash with water 3 times, centrifuge at 6000r / min for 20 minutes, discard the supernatant, and then wash with 40mmol / L boric acid The pH of the solution was adjusted to 6.6 to prepare the stock solution to be tested. Take 10ml of the stock solution to be tested, add 3.6g of urea and 0.05g of dithiothreitol (DTT), shake and mix to prepare the solution to be tested.

[0080] (2) Activation of uncoated capillary

[0081] Cut off an uncoated quartz capillary column with an in...

Embodiment 2

[0089] In this example, the kappa-casein content in raw milk was determined by the following steps:

[0090] (1) Raw milk pretreatment

[0091]Take 30ml of raw milk to be tested, put it in a 50ml centrifuge tube, and centrifuge at 3000r / min for 30 minutes to remove milk fat; then place it in a -40°C refrigerator for 20 minutes; take it out and return it to room temperature, and use 40mmol / L acetic acid aqueous solution to adjust the pH to 4.6, centrifuge at 3000r / min for 30 minutes, discard the supernatant, wash with water 3 times, centrifuge at 3000r / min for 30 minutes, discard the supernatant, and then wash with 40mmol / L boric acid The pH of the solution was adjusted to 6.6 to prepare the stock solution to be tested. Take 10ml of the stock solution to be tested, add 3.6g of urea and 0.05g of dithiothreitol (DTT), shake and mix to prepare the solution to be tested.

[0092] (2) Activation of uncoated capillary

[0093] Cut off an uncoated quartz capillary column with an i...

Embodiment 3

[0101] In this example, the kappa-casein content in raw milk was determined by the following steps:

[0102] (1) Raw milk pretreatment

[0103] Take 30ml of raw milk to be tested, put it in a 50ml centrifuge tube, and centrifuge at 10000r / min for 10 minutes to remove milk fat; then put it in a -40°C refrigerator for 10 minutes; take it out and return it to room temperature, and use 40mmol / L acetic acid aqueous solution to adjust the pH to 4.6, centrifuge at 3000r / min for 30 minutes, discard the supernatant, wash with water three times, centrifuge at 10000r / min for 10 minutes, discard the supernatant, and then wash with 40mmol / L boric acid The pH of the solution was adjusted to 6.6 to prepare the stock solution to be tested. Take 10ml of the stock solution to be tested, add 3.6g of urea and 0.05g of dithiothreitol (DTT), shake and mix to prepare the solution to be tested.

[0104] (2) Activation of uncoated capillary

[0105] Cut off an uncoated quartz capillary column with...

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Abstract

The invention proposes a reagent for coating capillaries and a method for determining the content of κ-casein in milk. According to an embodiment of the present invention, the capillary is used to determine the content of κ-casein in milk by capillary electrophoresis, and the reagent is an aqueous solution containing the following: 200mmol / L tris; 120mmol / L Hexaalkylammonium bromide; 80mmol / L polyethylene glycol; and 60mmol / L pentamethylenediamine, wherein the pH value of the reagent is 8.5. Therefore, the capillary measurement of κ-casein content obtained by using the reagent for coating the capillary in the present invention has at least one of the following advantages: good separation effect, short detection time, high repeatability and high accuracy.

Description

technical field [0001] The invention relates to the field of food. In particular, the present invention relates to a method for determining the content of kappa-casein in bovine milk. More specifically, the present invention relates to reagents for coating capillaries and methods for determining the content of kappa-casein in milk. Background technique [0002] κ-casein is the least protein in the casein components, accounting for about 12% of the total casein and 10% of the total protein in milk. The relative molecular mass of κ-casein is about 19000D, containing 169 amino acid residues and 1 phosphate group. [0003] However, the current method for determining the content of κ-casein in milk still needs to be improved. Contents of the invention [0004] The present invention aims to solve at least one of the technical problems existing in the prior art. Therefore, an object of the present invention is to provide a reagent for coating capillaries and a method for dete...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/447
Inventor 刘宇陈伟史玉东陈云母智深
Owner INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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