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Method for extracting and preparing solanesol from potato leaf

A technology of potato leaves and solanesol, which is applied in the field of extraction and preparation of solanesol, can solve the problems of small preparation throughput, and achieve the effects of cost reduction and simple process

Inactive Publication Date: 2015-12-16
ZHONGSHAN SENPU BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] There are many determination methods for solanesol, and there are a large number of reports at home and abroad, mainly including thin-layer chromatography, gas chromatography, thin-layer separation-coulometric titration, column chromatography separation gravimetric method, thin-layer scanning method, etc., but generally For analytical scale and semi-preparative scale, the preparative throughput is very small

Method used

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  • Method for extracting and preparing solanesol from potato leaf
  • Method for extracting and preparing solanesol from potato leaf
  • Method for extracting and preparing solanesol from potato leaf

Examples

Experimental program
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Embodiment 1

[0024] Crush the potato leaves through a 20-mesh sieve, extract by percolation with an ethanol solution 10 times the weight of the potato leaves for 6 hours, combine the ethanol extracts, recover the ethanol aqueous solution, concentrate until there is no alcohol smell, and obtain the ethanol extract; filter the ethanol extract HZ-801 macroporous resin, eluted with water, 40% ethanol, 60% ethanol, and 80% ethanol in sequence; the eluate after the last step above was eluted with 80% ethanol was collected, and the eluate prepared above Concentrate until there is no alcohol smell, and make a crude extract; the concentrated crude extract prepared above is dissolved in methanol solution successively, filtered through a 0.45 μm microporous membrane, separated by high performance liquid chromatography, and the column Shim-packPREP -ODS chromatographic column (20mm×250mm, 15μm), and then eluted with methanol-ethanol solution with a mobile phase ratio of methanol:ethanol (60:40, V / V) an...

Embodiment 2

[0026] The potato leaves are crushed through a 20-mesh sieve, extracted by percolation with an ethanol solution 5 times the weight of the potato leaves for 9 hours, and the ethanol extracts are combined. Recover the ethanol aqueous solution, concentrate until there is no alcohol smell, and obtain the ethanol extract; pass the ethanol extract through the macroporous resin HZ-801, and then elute with water, 40% ethanol, 60% ethanol, and 80% ethanol. Collect 80% ethanol eluate, and the obtained eluate is concentrated without alcohol smell. The concentrated crude extract was dissolved in methanol solution, filtered through a 0.45 μm microporous membrane, separated by preparative high-performance liquid chromatography, and chromatographic column Shim-packPREP-ODS column (20mm×250mm, 15 μm). The ratio of the mobile phase is: methanol: ethanol (60:40, V / V), the flow rate of the mobile phase is 10 mL / min, the ultraviolet detector is 210 nm for detection, and the injection volume is 30...

Embodiment 3

[0028] The potato leaves are crushed through a 20-mesh sieve, extracted by percolation with an ethanol solution 7 times the weight of the potato leaves for 8 hours, and the ethanol extracts are combined. Recover the ethanol aqueous solution, concentrate until there is no alcohol smell, and obtain the ethanol extract; pass the ethanol extract through the macroporous resin HZ-801, and then elute with water, 40% ethanol, 60% ethanol, and 80% ethanol. Collect 80% ethanol eluate, and the obtained eluate is concentrated without alcohol smell. The concentrated crude extract was dissolved in methanol solution, filtered through a 0.45 μm microporous membrane, separated by preparative high-performance liquid chromatography, and chromatographic column Shim-packPREP-ODS column (20mm×250mm, 15 μm). The ratio of the mobile phase is: methanol: ethanol (60:40, V / V), the flow rate of the mobile phase is 10 mL / min, the ultraviolet detector is 210 nm for detection, and the injection volume is 30...

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Abstract

The invention provides a method for extracting and preparing solanesol from potato leaf. The method comprises the following steps: leaching the medicinal material powder with ethanol at 80 DEG C under reflux, concentrating the extracting solution, and carrying out two-step high-efficiency purification by macroporous resin column separation and preparative high performance liquid chromatography to obtain the solanesol with the purity of greater than 98%. The method has the advantages of simple technical steps and high purity, and can easily implement scale-up production.

Description

Technical field: [0001] The invention relates to a method for extracting and preparing solanesol from potato leaves. Background technique: [0002] Solanesol is an important raw material for drug synthesis and an irreplaceable key intermediate for the synthesis of ubiquinone drugs. Coenzyme Q10, which has been produced and widely used in many developed countries, must rely on high-purity solanesol as a raw material. Coenzyme Q10 is widely used in the fields of medicine, health care and cosmetics. In recent years, with the increase in the recommended dosage of coenzyme Q10 and the increasing demand in the international market, the demand for solanesol in the international market is also increasing day by day. Therefore, the preparation of high-purity solanesol is of great significance for the in-depth study of its pharmacological effects and related applications. [0003] There are many methods for the determination of solanesol, and there are a large number of reports at h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C29/76C07C33/02
CPCC07C29/76C07C33/02
Inventor 梁勇黄宁何波
Owner ZHONGSHAN SENPU BIOTECH CO LTD
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