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Dehydrated protein gene for medicago ruthenica (L.) and application thereof

A technology of dehydrating protein and flat clover beans, applied in the fields of application, genetic engineering, plant gene improvement, etc., can solve the problems of no stress resistance function identification, etc., and achieve the effect of improving salt resistance and heat resistance

Inactive Publication Date: 2015-12-16
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are only sporadic reports on the cloning of the dehydrin gene of fennel bean, but there is no report on the identification of its stress resistance function

Method used

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  • Dehydrated protein gene for medicago ruthenica (L.) and application thereof
  • Dehydrated protein gene for medicago ruthenica (L.) and application thereof
  • Dehydrated protein gene for medicago ruthenica (L.) and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Ledder bean dehydrated protein gene, the gene is Mrdhn3 Coding genes with a nucleotide sequences shown in the first to 890 bits in seqidno.1 in an orderly list.

[0035] 1. Ledar bean dehydrated protein Mrdhn3 The cloning of genes.

[0036] 1. Low temperature coercion treatment and transcription group sequencing of lentil bean seedlings:

[0037] ⑴ Low -temperature coercion treatment:

[0038] After 10 minutes of concentrated sulfuric acid with concentrated sulfur, remove the residual sulfuric acid several times with distilled water.The above -mentioned seedlings are placed in a double -layer filter paper dine, germinating under the condition of 21 ° C and 16H / 8H light cycle; after 3D, the seedlings that germinate are transplanted to vermiculite: nutritional soil (3: 1) mixingContinue to cultivate under the above conditions in the matrix, and pour it with 1 / 2MS nutrient solution once a week; transfer the seedlings to -4 ℃ for light treatment box for low temperatur...

Embodiment 2

[0120]Example 2 Ledar bean dehydrated protein gene, the gene is Mrdhn3 Coding genes, which have functional activity genes formed by the lack of one or more alkali bases in the order of seqidno.1 in the order of order or more alkali.

[0121] Ledar bean dehydrated protein Mrdhn3 Calm and lenticon dehydration of genes of genes Mrdhn3 The core expression of genes of genes is the same as the embodiment 1.

Embodiment 3

[0122] Example 3 Ledar bean dehydrated protein gene, the gene is Mrdhn3 Code gene, it has figure 2 The protein of amino acid residue sequences shown in SEQIDNO.2 in the sequences and sequences.

[0123] Ledar bean dehydrated protein Mrdhn3 Calm and lenticon dehydration of genes of genes Mrdhn3 The core expression of genes of genes is the same as the embodiment 1.

[0124] right Mrdhn3 The protein product encoded in the CDNA code box is composed of 221 amino acid residues, and the molecular weight ( MW ) It is 24.84kd, and the theoretical point (PI) is 5.56, which is acidic protein.The total average hydrophobic index Grandaverageofhydropathicity (Gravy) is -1.444, which has high hydrophilicity.

[0125] PFAM database (http: / / pfam.janelia.org) database searches the domain of the amino acid sequence encoded by mrdhn3, indicating that MRDHN3 protein belongs to the Leaii protein family, and there is a S-fragment (solid wire frame) and 2 rich.K-fragments containing lysine (labeled in th...

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Abstract

The invention relates to a dehydrated protein gene for medicago ruthenica (L.). The gene is an MrDHN3 encoding gene and comes from the medicago ruthenica (L.). The gene has a nucleotide sequence shown as SEQ ID NO. 1 in a sequence table, or has an allele with functional activity, or has protein of an amino acid residue sequence shown as SEQ ID NO. 2 in the sequence table, or has protein which comprises an S-fragment including a series of serine (Ser) residues and two K-fragments (EKKGIMDKIKEKLPG or derivatives of the fragments) rich in lysine residues, wherein the allele is formed by deficiency or insertion or replacement of one or more basic groups in the SEQ ID NO. 1 of the sequence table, the protein is formed in the way that an amino acid residue sequence shown in the SEQ ID NO. 2 of the sequence table are subjected to replacement or deficiency or addition through one or more amino acid residues, and the protein has the same activity as the amino acid residue sequence shown in the SEQ ID NO. 2 and is derived through the SEQ ID NO. 2. The obtained gene can be used for improving the salt resistance and heat resistance of plants and microorganisms.

Description

Technical field [0001] The present invention involves the field of biological gene engineering technology, especially the lenticon dehydration protein gene and its application. Background technique [0002] Desert protein belongs to the Leaii family of late embryos (LateembryogenesisSabundantprotein).Desert protein was initially discovered in the seeds. Subsequent studies showed that dehydrated protein was widely distributed in various tissues and organs of algae, yeast, nematodes, blue bacteria, and high plants.Drought, low -temperature and high salt, etc., which cause dehydration and shed acid (ABA), can induce their expression in cells.Desert protein contains at least one conservative lysine-rich base-based k-fragment (Ekkgimdkikeklpg or its derivatives), which often forms a spiral structure of bilateral (hydrophilic lipids).Some dehydrated proteins usually include Y-fragments and S-fragments. Y-fragments usually appear in the N-end of dehydrated protein in 1-3 repeated forms ...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415A01H5/00
CPCY02A40/138
Inventor 王海庆沈迎芳窦全文陈志国
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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