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Method for extracting compound amino acid from polygonatum odoratum through enzymolysis and ultrasonic treatment

An amino acid and ultrasonic technology, applied in the biological field, can solve the problems of the amino acid extraction rate not reaching the ideal effect, the extraction process conditions not reaching the optimal value, the destruction of the functional components of the extracted material, etc. effect of operation

Inactive Publication Date: 2015-12-16
CHONGQING DOUHAO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the commonly used methods for extracting amino acids include acid-base extraction, heating reflux extraction, leaching, etc. The extraction solvents are generally water, alcohol, ether, etc. The extraction rate of these methods is not high and the functional components of the extracted substances are destroyed, and the effect is not good. ideal
Ultrasonic extraction is a newly developed new technology, which can not only improve the extraction rate, but also protect the functional components of the extracted substance, but the extraction process conditions such as ultrasonic power, extraction time, concentration of extractant, and solid-liquid ratio affect the extraction rate. The optimal value was not reached, resulting in the extraction rate of amino acids not reaching the desired effect
[0004] At present, no patent has been found to extract amino acids from Polygonatum odoratum by combined enzymatic hydrolysis and ultrasonic treatment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A method for extracting amino acids from Polygonatum Polygonatum by enzymolysis ultrasound, comprising the following steps:

[0037] (1) Pretreatment: wash Polygonatum odoratum, drain the water, dry at 60-80°C, then cut into small pieces, pulverize with a pulverizer, and pass through a 40-mesh sieve to obtain Polygonatum odoratum powder for later use;

[0038] (2) Ultrasonic treatment: Take Polygonatum odoratum powder obtained in step (1) and add water or organic solvent according to the ratio of solid-liquid ratio of 1g:15mL, and ultrasonically treat for 45min at a power of 250W;

[0039] (3) Decolorization: add activated carbon to the solution obtained in step (2) for decolorization, the mass volume ratio of activated carbon to the solution is 1g:120mL, treat at 100rpm at 75°C for 35min, filter, and concentrate the filtrate to 1 / 5 of the original volume under reduced pressure ;

[0040] (4) Impurity removal: Add absolute ethanol to the solution obtained in step (3), ...

Embodiment 2

[0046] A method for extracting amino acids from Polygonatum Polygonatum by enzymolysis ultrasound, comprising the following steps:

[0047] (1) Pretreatment: wash Polygonatum odoratum, drain the water, dry at 60-80°C, then cut into small pieces, pulverize with a pulverizer, and pass through a 40-mesh sieve to obtain Polygonatum odoratum powder for later use;

[0048] (2) Ultrasonic treatment: Take Polygonatum odoratum powder obtained in step (1) and add water or organic solvent according to the ratio of solid-liquid ratio of 1g:10mL, and ultrasonically treat for 40min at a power of 250W;

[0049] (3) Decolorization: add activated carbon to the solution obtained in step (2) for decolorization, the mass volume ratio of activated carbon to the solution is 1g:100mL, treat at 100rpm at 70°C for 30min, filter, and concentrate the filtrate under reduced pressure to 1 / 5 of the original volume ;

[0050] (4) Impurity removal: add absolute ethanol to the solution obtained in step (3), ...

Embodiment 3

[0055] A method for extracting amino acids from Polygonatum Polygonatum by enzymolysis ultrasound, comprising the following steps:

[0056] (1) Pretreatment: wash Polygonatum odoratum, drain the water, dry at 60-80°C, then cut into small pieces, pulverize with a pulverizer, and pass through a 40-mesh sieve to obtain Polygonatum odoratum powder for later use;

[0057] (2) Ultrasonic treatment: Take Polygonatum odoratum powder obtained in step (1) and add water or organic solvent according to the ratio of solid to liquid ratio of 1g:20mL, and ultrasonically treat for 50min at a power of 250W;

[0058] (3) Decolorization: add activated carbon to the solution obtained in step (2) for decolorization, the mass volume ratio of activated carbon to the solution is 1g:150mL, treat at 100rpm at 80°C for 40min, filter, and concentrate the filtrate under reduced pressure to 1 / 5 of the original volume ;

[0059] (4) Impurity removal: add absolute ethanol to the solution obtained in step (3...

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Abstract

The invention discloses a method for extracting compound amino acid from polygonatum odoratum through enzymolysis and ultrasonic treatment. Polygonatum odoratum is used as a raw material. The method comprises the concrete steps of (1) pretreating; (2) carrying out enzymolysis and ultrasonic treatment; (3) decolorizing; (4) removing impurities; (5) purifying; and (6) dissolving a crystal, adding activated carbon, stirring and filtering, placing the solution into a refrigerator to filter, separating out an amino acid crystal, and drying to obtain the compound amino acid. The method has the beneficial effects that the compound amino acid is extracted from polygonatum odoratum because a compound enzyme and ultrasonic waves are combined to act on the polygonatum odoratum, the acting condition of the enzyme is mild, and the enzyme also plays a role in breaking a wall, so that the amino acid in the polygonatum odoratum is thoroughly dissolved out; the ultrasonic wave extraction process is a physical process, so that no chemical changes are generated in the extraction process, the extraction ratio is high, functional components of an extract are effectively protected, and the breaking time is shortened; and the method disclosed by the invention is simple and easy to operate and suitable for industrial production.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for extracting amino acids from Polygonatum Polygonatum by enzymatic hydrolysis and ultrasound. Background technique [0002] Polygonatum odoratum, also known as bell dish, tail ginseng, ground pipe, sweet grass root, is a perennial herb of Liliaceae. Modern pharmacological research results show that the rhizome of Polygonatum Polygonatum has the effects of expanding coronary arteries, lowering blood fat, lowering blood sugar, improving liver microcirculation, anti-tumor, anti-aging and enhancing immunity, and is a nourishing and strengthening agent. Polygonatum Polygonatum is rich in trace elements, vitamins, amino acids, steroidal saponins, flavonoids, alkaloids, polysaccharides and other components, and amino acids, as the basic unit of protein, directly participate in the metabolism and other physiological activities in organisms, and can be used in medicine as Nutrient...

Claims

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Application Information

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IPC IPC(8): C12P13/04
Inventor 宁发子张娜罗雪文
Owner CHONGQING DOUHAO BIOTECH