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The preparation method of l-aspartic acid-l-ornithine

A technology of aspartic acid and ornithine, which is applied in the direction of fixing on/in the organic carrier, fermentation, etc., can solve the problems of strong alkalinity, long time and high cost of enzymatic hydrolysis, and achieve mild reaction conditions and high conversion The effect of short time and low product cost

Active Publication Date: 2018-11-09
JING JING PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of this method are: the product yield is low (about 50%), and a large amount of resin is consumed, and a large amount of water is consumed in the resin regeneration process, and a large amount of difficult acid water to be treated is produced, and a large amount of three wastes is generated , the cost is high, and it is difficult to realize industrial production
The patent enzymatic hydrolysis has strong alkalinity, high temperature and long time, which will lead to the polymerization of ornithine to produce impurities

Method used

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  • The preparation method of l-aspartic acid-l-ornithine
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  • The preparation method of l-aspartic acid-l-ornithine

Examples

Experimental program
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Effect test

Embodiment 1

[0043] (1) Preparation of immobilized enzyme: ultra-large pore polystyrene microspheres, washed with deionized water and filtered as a carrier; take 0.08g of L-arginase, add deionized water to dissolve to 40ml, add Na 2 HPO4-NaH 2 20ml of PO4 buffer solution, stirred and heated to 36°C±1°C, added 5.0g of carrier, stirred for 18 hours, filtered and washed with deionized water to obtain immobilized enzyme, refrigerated at 4°C to 8°C; The average particle size of the ball is 100 microns, the porosity is 60%, and the specific surface area is 34m 2 / g, degree of cross-linking 12%; Na 2 HPO4-NaH 2 The concentration of PO4 buffer solution is 0.1mol / L, pH9.0.

[0044] (2) Ingredients, enzymatic hydrolysis reaction:

[0045] Ingredients: Add 20g of L-arginine and 40mg of manganese sulfate into 60ml of deionized water, stir to dissolve, add 6M hydrochloric acid to adjust the pH value to 8.5, add deionized water to make the solution volume 100ml, and then add 4g of immobilized enzyme...

Embodiment 2

[0050] (1) Preparation of immobilized enzyme: ultra-large pore polystyrene microspheres, washed with deionized water and filtered as a carrier; take 0.05g of L-arginase, add deionized water to dissolve to 40ml, add Na 2 HPO4-NaH 2 20ml of PO4 buffer solution, stirred and heated to 36°C±1°C, added 5.0g of carrier, stirred for 20 hours, filtered and washed with deionized water to obtain immobilized enzyme, refrigerated at 4°C to 8°C; The average particle size of the ball is 200 microns, the porosity is 50%, and the specific surface area is 40m 2 / g, degree of cross-linking 15%; Na 2 HPO4-NaH 2 The concentration of PO4 buffer solution is 0.05mol / L, pH8.5.

[0051] (2) Ingredients, enzymatic hydrolysis reaction:

[0052] Ingredients: Add 15g of L-arginine and 22.5mg of manganese sulfate into 60ml of deionized water, stir to dissolve, add 6M hydrochloric acid to adjust the pH value to 8.5, add deionized water to make the solution volume 100ml, and then add 4g of immobilized enz...

Embodiment 3

[0057] (1) Preparation of immobilized enzyme: ultra-large pore polystyrene microspheres, washed with deionized water and filtered as a carrier; take 0.06g of L-arginase, add deionized water to dissolve to 40ml, add Na 2 HPO4-NaH 2 20ml of PO4 buffer solution, stir and heat up to 36°C±1°C, add 5.0g carrier, stir for 22 hours, filter and wash with deionized water to obtain immobilized enzyme, refrigerated at 4°C to 8°C; The average particle size of the ball is 100 microns, the porosity is 60%, and the specific surface area is 34m 2 / g, degree of cross-linking 12%; Na 2 HPO4-NaH 2 The concentration of PO4 buffer solution is 0.2mol / L, pH8.8.

[0058] (2) Ingredients, enzymatic hydrolysis reaction:

[0059] Ingredients: Add 10g of L-arginine and 30mg of manganese sulfate into 60ml of deionized water, stir to dissolve, add 6M hydrochloric acid to adjust the pH value to 8.5, add deionized water to make the solution volume 100ml, and then add 4g of immobilized enzyme.

[0060] En...

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Abstract

The invention discloses a preparation method of L-aspartate-L-ornithine and relates to the technical field of preparation of two amino acid known sequences. The method includes the following steps that an ultra-large-hole polystyrene microsphere is used, filtering is performed after deionized water is added to wash the ultra-large-hole polystyrene microsphere, the washed ultra-large-hole polystyrene microsphere serves as a carrier, and arginase is fixed to the carrier; then, L-arginine is added into deionized water, a catalyst of manganese sulfate and manganese acetate is added, stirring is performed so that the L-arginine can be dissolved, the pH value is adjusted to 8.3-8.7 through hydrochloric acid or sulfuric acid, an L-arginine solution is obtained, immobilized enzyme is added, enzymatic hydrolysis is performed, and transformation liquid is obtained through filtering; then the transformation liquid is subjected to adsorbing elution through strong-acid cation exchange resin and strong-basicity anion exchange resin, and an L-ornithine solution is obtained; the L-ornithine solution and L-aspartate have a salt forming reaction, and an L-aspartate-L-ornithine solution is obtained; finally, decoloration and crystallization are performed so that the product can be obtained. According to the preparation method, reaction conditions are moderate, the L-aspartate-L-ornithine yield is high, cost is low, and purity is high.

Description

technical field [0001] The invention relates to the technical field of preparation of a known sequence with two amino acids. Background technique [0002] L-Aspartic acid-L-ornithine, the chemical name is (S)-2-aminosuccinic acid-(S)-2,5-diaminovalerate, the molecular formula is C 9 h 19 N 3 o 6 , the molecular weight is 265.26, and the structural formula is: [0003] [0004] L-aspartic acid-L-ornithine is mainly used for the treatment of elevated blood ammonia caused by hepatic encephalopathy and acute and chronic liver diseases (such as various types of hepatitis, liver cirrhosis, fatty liver, post-hepatitis syndrome), such as accompanied by It is especially suitable for treating the early stage of hepatic coma or the confusion state of hepatic coma when the detoxification function of the liver is impaired or hepatic encephalopathy occurs. [0005] In the existing preparation method of L-aspartic acid-L-ornithine, L-ornithine mostly exists in the form of hydrochlo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/02C12N11/08
Inventor 李斌水何瑞红赵玉红张玉峰
Owner JING JING PHARMA