Method for extracting tobacco oil component through ultrasonic assisted Soxhlet extraction method

A Soxhlet extraction method and ultrasonic-assisted technology, which is applied in the field of tobacco component extraction, can solve the problems such as ineffective effects, and achieve the effects of less solvent consumption, improved oil extraction rate, and simple operation

Active Publication Date: 2015-12-16
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the method of extracting tobacco oil with ultrasonic method as pretreatment is common, the effect is not obvious

Method used

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  • Method for extracting tobacco oil component through ultrasonic assisted Soxhlet extraction method
  • Method for extracting tobacco oil component through ultrasonic assisted Soxhlet extraction method
  • Method for extracting tobacco oil component through ultrasonic assisted Soxhlet extraction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] In order to further understand the present invention, the above-mentioned technical solutions will be further elaborated and described below in conjunction with specific implementation modes.

[0029] See Table 1 and Table 2 for the specific conditions of the reagents and equipment used in the following examples.

[0030] name purity manufacturer Dichloromethane Analytical Pure (AR) Tianjin Fengchuan Chemical Reagent Technology Co., Ltd. Anhydrous Sodium Sulfate Analytical Pure (AR) Tianjin Yaohua Chemical Reagent Co., Ltd. Sodium chloride Analytical Pure (AR) Tianjin Kemiou Chemical Reagent Co., Ltd.

[0031] name manufacturer RE-2000B rotary evaporator Gongyi Yuhua Instrument Co., Ltd. SHZ-D(Ⅲ) circulating water vacuum pump Henan Yuhua Instrument Co., Ltd. FW-400A inclined high-speed universal pulverizer Beijing Zhongxing Weiye Instrument Co., Ltd. electronic analytical balance Beijing...

Embodiment 2

[0064] Repeat the specific extraction steps of tobacco oil in Example 1 to extract four different tobacco samples from Pingdingshan C032, Fujian Nanping C015, Luoyang Luoning C3F and Zambia ZM-BOAT, and use GC / MS instrument to detect the components of the extracts. The components detected in four different samples were compared and analyzed.

[0065] GC / MS combined instrument and detection conditions

[0066] The gas chromatography conditions are:

[0067] Chromatographic column: HP-5MS (30m×0.25mm×0.25μm)

[0068] Programmed temperature rise: keep at 60°C for 5 minutes, raise to 135°C at a rate of 4°C / min; rise to 175°C at a rate of 1°C / min; then raise to 310°C at a rate of 10°C / min, and hold for 10 minutes.

[0069] Injection port temperature: 250°C, injection volume 1μL; carrier gas is high-purity He, carrier gas flow rate 1ml / min; solvent delay 6min; split ratio 10:1

[0070] Mass spectrometry detection conditions:

[0071] EI ion source temperature: 230°C; ion source ...

example 2

[0073] Table 3 Composition of oil in tobacco

[0074]

[0075] Continued Table 3 Composition of Oil in Tobacco

[0076]

[0077] Continued Table 3 Composition of Oil in Tobacco

[0078]

[0079] Continued Table 3 Composition of Oil in Tobacco

[0080]

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Abstract

The present invention discloses a method for extracting the tobacco oil component through an ultrasonic assisted Soxhlet extraction method. The method comprises: 1) weighing tobacco with a certain mesh number, loading into a filtration paper bag, drying, and cooling; 2) placing the filtration paper bag in the step 1) into the receiving bottle of a Soxhlet extraction device, and soaking for a certain time with a certain amount of petroleum ether; 3) treating the receiving bottle in the step 2) through an ultrasonic method; 4) carrying out Soxhlet extraction; 5) concentrating the petroleum ether extraction solution; and 6) detecting and analyzing the tobacco oil component through GC/MS. According to the present invention, compared with the standard method (YC/T176-2003), the process of the present invention has the following characteristics that: the total amount of the petroleum ether extract is averagely increased by more than or equal to 15%, the precision and the reproducibility are significant, the solvent consumption is less, and the operation is simple.

Description

technical field [0001] The invention belongs to the technical field of tobacco component extraction, and relates to the extraction of oil in tobacco, in particular to a method for extracting tobacco oil by ultrasonic-assisted Soxhlet extraction. Background technique [0002] Tobacco is the most widely planted economic crop in the world, and its chemical composition mainly includes six substances including total sugar, total nitrogen, reducing sugar, nicotine, potassium and chlorine. Tobacco oil is a kind of aroma-inducing substance with the characteristic aroma of tobacco, and it is an ideal additive for tobacco products. Its content directly reflects the quality of tobacco leaves, and increasing the oil content can improve the quality of cigarettes. Tobacco leaf oil is fully separated, on the one hand, it can maintain the "color, aroma, and taste" of cigarettes, and on the other hand, it can reduce the harm of tobacco to human health. [0003] Oil content is a general conc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06G01N30/12
Inventor 吴鸣建韦凤杰刘文涛李国富游霞马川董坤乐苏新宏李翀
Owner ZHENGZHOU UNIV
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