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A method that can simultaneously evaluate the quality of four raw materials of traditional Chinese medicine compound preparations for nourishing blood

A technology for compound preparations and traditional Chinese medicines, applied in the field of quality evaluation of Chinese medicinal materials, can solve the problems of inconsistency in the liquid phase conditions of the composition analysis methods of medicinal materials, inability to fully reflect the quality, long extraction time, etc., and achieve simple and accurate pretreatment methods for medicinal materials Evaluation, good effect of stability

Active Publication Date: 2018-01-16
SHAN DONG DONG E E JIAO
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The ingredients in the four Chinese medicinal materials need to be extracted separately with different reagents, the method is cumbersome, the extraction time is long, and the liquid phase conditions of the analysis methods of the ingredients of each medicinal material are inconsistent;
[0005] In the Pharmacopoeia, the detection of the components of hawthorn is a single component of citrate, and the detection of the active components of red ginseng is three components of Rg1, Re, and Rb1, which cannot fully reflect the quality of these two medicinal materials.

Method used

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  • A method that can simultaneously evaluate the quality of four raw materials of traditional Chinese medicine compound preparations for nourishing blood
  • A method that can simultaneously evaluate the quality of four raw materials of traditional Chinese medicine compound preparations for nourishing blood
  • A method that can simultaneously evaluate the quality of four raw materials of traditional Chinese medicine compound preparations for nourishing blood

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Quality inspection of four kinds of raw medicinal materials

[0029] (1), the preparation of test sample

[0030] Take 5 batches of Rehmannia glutinosa, Codonopsis pilosula, hawthorn, and red ginseng medicinal materials from Henan, Shanxi, Gansu and other places, accurately weigh 2 g of Codonopsis pilosula powder that has been crushed and passed through a No. 4 sieve, and place it in a conical flask;

[0031] Cut Rehmannia glutinosa into slices with a thickness of 0.5 cm, dry in an oven at 60°C for half an hour, crush them, pass through a 40-mesh sieve, accurately weigh 5 g of rehmannia glutinosa powder, 2 g of hawthorn powder, and 5 g of red ginseng powder and place them in the In the Erlenmeyer flask, add 100ml of 75% methanol and sonicate twice, each time for 0.5 hours. After filtration, the filtrate is combined, and the filtrate is diluted 20 times with methanol, as the test solution.

[0032] (2) Preparation of standard product: Accurately weigh tangshenoside, erg...

Embodiment 2

[0056] repeatability test

[0057] Precisely weigh rehmannia glutinosa, Codonopsis pilosula, hawthorn, red ginseng medicinal materials, and make test solution samples after the medicinal material pretreatment method in the above-mentioned test scheme of the present invention is processed,

[0058] (1), the preparation of test sample

[0059] Accurately weigh 2 g of Codonopsis pilosula powder that has been crushed through a No. 4 sieve, and place it in a conical flask;

[0060] Cut Rehmannia glutinosa into slices with a thickness of 0.5 cm, dry in an oven at 60°C for half an hour, crush them, pass through a 40-mesh sieve, accurately weigh 5 g of rehmannia glutinosa powder, 2 g of hawthorn powder, and 5 g of red ginseng powder and place them in the In the Erlenmeyer flask, add 100ml of 75% methanol and sonicate twice, each time for 0.5 hours. After filtration, the filtrate is combined, and the filtrate is diluted 20 times with methanol, as the test solution.

[0061] (2) Prepa...

Embodiment 3

[0082] Sample recovery test

[0083] (1), the preparation of test sample

[0084] Accurately weigh 2 g of Codonopsis pilosula powder that has been crushed through a No. 4 sieve, and place it in a conical flask;

[0085] Cut rehmannia glutinosa into slices with a thickness of 0.5 cm, dry in an oven at 60°C for half an hour, crush them, pass through a 40-mesh sieve, accurately weigh 5 g of rehmannia glutinosa powder, 2 g of hawthorn powder, and 5 g of red ginseng powder and place them in the same Erlenmeyer flask. Accurately weigh 6 parts of Rehmannia glutinosa powder, Codonopsis powder, hawthorn powder, and red ginseng powder, add 80ul of mixed standard solution respectively, add 100ml of 75% methanol and ultrasonicate twice, each time for 0.5 hours, filter and combine the filtrates, and dissolve them with methanol The filtrate was diluted 20 times as the test solution.

[0086] (2) Preparation of standard product: Accurately weigh tangshenoside, ergosteroside, citric acid, ...

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Abstract

The invention discloses a method capable of simultaneously evaluating the quality of four kinds of raw medicinal materials in a blood-enriching traditional Chinese medicine compound preparation. The method can simultaneously evaluate the quality of the four medicinal materials. Compared with the traditional method of the Pharmacopoeia, a more comprehensive and convenient comprehensive evaluation method is proposed. The present invention uses the HPLC-MS method to determine a blood-enriching traditional Chinese medicine compound preparation—four raw medicinal materials in compound donkey-hide gelatin pulp—codonopsis, rehmannia glutinosa, hawthorn, and tangshenin, ergosteroside, citrate, and chlorogenic acid in red ginseng. , Rg1, Re, Rb1, S-Rg2, R-Rg2 content, realized the quantitative detection of four kinds of medicinal materials without separate treatment, and one sample injection to nine kinds of medicinal ingredients; Pretreatment conditions, chromatographic conditions, mobile phase composition, gradient elution conditions, mass spectrometry conditions, etc., have been proved by methodological investigation and multi-batch verification that the multi-index system evaluation method provided by the present invention has good stability, repeatability and precision. The quality of the original medicinal materials of the compound preparation can be evaluated more comprehensively and accurately.

Description

technical field [0001] The invention relates to a method for evaluating the quality of traditional Chinese medicinal materials, in particular to a method for evaluating the quality of four original medicinal materials in a compound preparation of blood-enriching traditional Chinese medicine. Background technique [0002] Compound donkey-hide gelatin paste is a compound traditional Chinese medicine oral liquid for nourishing qi and blood produced by Dong'e donkey-hide gelatin Co., Ltd. Its formula is composed of donkey-hide gelatin, rehmannia glutinosa, Codonopsis pilosula, hawthorn and red ginseng. At present, the quality standard test items of raw medicinal materials need to separately measure the content of each medicinal component in the five medicinal materials. Each medicinal material needs to be processed separately and sampled separately for testing, and the currently established component determination indicators cannot fully reflect the quality of medicinal materials...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/06
Inventor 张淹赵婷婷周祥山王春艳李士栋张路彭照琪孙阳恩
Owner SHAN DONG DONG E E JIAO
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