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A kind of preparation method and application of total polyphenols

A technology for total polyphenols of orchid isophylla is applied in the field of preparation of total polyphenols of blue orchid, which can solve the problems such as no research reports on polyphenolic compounds of blue orchid, and achieve easy large-scale production, good purification effect and enrichment efficiency. high effect

Inactive Publication Date: 2019-09-10
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no research report on the polyphenolic compounds of P.

Method used

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  • A kind of preparation method and application of total polyphenols
  • A kind of preparation method and application of total polyphenols
  • A kind of preparation method and application of total polyphenols

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Experimental program
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Effect test

Embodiment 1

[0051] Embodiment 1 A kind of preparation method of total polyphenols of P. heterophyllum comprises the following steps:

[0052] ⑴Extraction: 1 kg of Qinglan herb powder is refluxed once at 50°C with 5 times its mass and 90% volume concentration of ethanol aqueous solution, and the extract is obtained after 3 hours. The extract is extracted at a temperature of 50°C and a pressure of Concentrate under reduced pressure under the condition of 0.07MPa to obtain 120.4g of the ethanol extract of Indigo indica.

[0053] Among them: the whole herb powder of Qinglan heterophylla refers to the powder obtained by passing Qinglan different leaves collected from Haibei Prefecture, Guoluo Prefecture, Yushu Prefecture and Haidong area of ​​Qinghai Province after drying and crushing in the shade, and passing through a 40-mesh sieve.

[0054] (2) Removal of pigment: Dissolve the ethanol extract of Indigo isophyllum in distilled water 30 times its mass, add petroleum ether equal to the volume ...

Embodiment 2

[0060] Embodiment 2 A preparation method of total polyphenols of P. heterophylla, comprising the following steps:

[0061] ⑴Extraction: 1kg of Qinglan herb powder is refluxed and extracted twice at 60°C with 10 times its mass and 70% volume concentration of ethanol aqueous solution, each time for 4 hours, and combined to obtain the extract, which is extracted at a temperature of 60°C and concentrated under reduced pressure under the condition of 0.07MPa to obtain 218.3g of the ethanol extract of P.

[0062] Among them: the whole herb powder of Qinglan heterophylla refers to the powder obtained by passing Qinglan different leaves collected from Haibei Prefecture, Guoluo Prefecture, Yushu Prefecture and Haidong area of ​​Qinghai Province after drying and crushing in the shade, and passing through a 40-mesh sieve.

[0063] (2) Removal of pigment: Dissolve the ethanol extract of Indigo isophyllum with 30 times the mass of distilled water, add petroleum ether equal to the volume of...

Embodiment 3

[0069] Embodiment 3 A preparation method of total polyphenols of P. heterophyllum comprises the following steps:

[0070] ⑴Extraction: 2kg of Qinglan herb powder is refluxed and extracted twice at 60°C with 10 times its mass and 70% volume concentration of ethanol aqueous solution, each time for 5h, and combined to obtain an extract, which is extracted at a temperature of 70°C 1. Under the condition of 0.06 MPa, the pressure was concentrated under reduced pressure to obtain 541.4 g of the ethanol extract of P. heterophyllum.

[0071] Among them: the whole herb powder of Qinglan heterophylla refers to the powder obtained by passing Qinglan different leaves collected from Haibei Prefecture, Guoluo Prefecture, Yushu Prefecture, and Haidong area of ​​Qinghai Province after being dried and crushed in the shade, and then passed through a 60-mesh sieve.

[0072] (2) Removal of pigment: Dissolve the ethanol extract of Indigo isophyllum in distilled water 50 times its mass, add petrole...

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Abstract

The invention relates to a method for preparing total polyphenol of dracocephalum heterophyllum benth. The method comprises the following steps: (1) extracting, namely performing alcohol extraction and decompression concentration on dracocephalum heterophyllum benth herbal powder to obtain a dracocephalum heterophyllum benth alcohol extract; (2) removing pigment, namely dissolving the dracocephalum heterophyllum benth alcohol extract, and extracting the dracocephalum heterophyllum benth alcohol extract to obtain a water extract; (3) crudely preparing, namely performing extraction and decompression concentration on the water extract to obtain a dracocephalum heterophyllum benth crude product; and (4) finely preparing, namely dissolving the dracocephalum heterophyllum benth crude product into a suspension, regulating the pH value of the suspension to be 3-4 with a HCl solution of 0.5mol / L, eluting the solution with a processed macroporous resin chromatography column by adopting a wet method, and collecting 50-70% alcohol water eluent; and performing decompression concentration on the 50-70% alcohol water eluent to obtain a finished product of the total polyphenol of dracocephalum heterophyllum benth.

Description

technical field [0001] The invention relates to a preparation method of total polyphenols, in particular to a preparation method and application of total polyphenols of P. heterophyllum. Background technique [0002] different leaf green orchid ( Dracocephalum heterophyllum Benth) belongs to Labiatae ( Labiatae ) Cymbidium ( Dracocephalum ) plants, mainly distributed in temperate Asia. The plant is widely used in the folk, and has the effects of calming the liver, clearing away heat, and treating high blood pressure, lymphadenitis, and cough due to lung heat. Modern studies have shown that the chemical constituents in the different leaves mainly include phenylpropanoids, flavonoids and terpenes. So far, there is no research report on the polyphenolic compounds of P. isophyllum. [0003] Studies have shown that: the strong oxidation of free radicals can lead to gene mutations, cell damage, pigment formation, and tissue hardening. Human aging, cardiovascular and cerebr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/53A61P39/06A23L33/105
Inventor 王启兰史强强党军张莉陶燕铎王蒲卢晓华郑威
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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