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Method and kit for detecting staphylococcus aureus

A staphylococcus and golden yellow technology, applied in the field of microbial detection, can solve the problems of high technical ability requirements of operators and the value of popularization and application to be studied, and achieve the effects of high sensitivity and consistency, ability to inhibit miscellaneous bacteria, and easy results

Active Publication Date: 2015-12-23
蒙牛乳业(太原)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] There are many detection methods for Staphylococcus aureus, such as: microbiological methods, biological methods, immunological methods, gene probe technology, thermostable nuclease method, biosensing technology, superantigen technology, polymerase chain reaction technology, these Among detection technologies, except for microbiological methods, on the one hand, other detection technologies are only in the research and development stage, and the value of popularization and application needs to be studied; high

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  • Method and kit for detecting staphylococcus aureus
  • Method and kit for detecting staphylococcus aureus
  • Method and kit for detecting staphylococcus aureus

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Experimental program
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Embodiment

[0028] 1. Equipment and materials

[0029] (1) Constant temperature incubator: 36°C±1°C.

[0030] (2) Refrigerator: 2°C to 5°C.

[0031] (3) Constant temperature water bath: 37℃~65℃.

[0032] (4) Balance: Sensitivity 0.1g.

[0033] (5) Homogenizer (rotary knife type or clapping type).

[0034] (6) Oscillator.

[0035] (7) Sterile pipette: 1mL (with 0.01mL scale), 10mL (with 0.1mL scale), or micropipette and tip.

[0036] (8) Sterile Erlenmeyer flask: capacity 100mL, 500mL.

[0037] (9) Sterile petri dish: 90 mm in diameter.

[0038] (10) Syringe: 0.5mL.

[0039] (11) PH meter or precision PH test paper.

[0040] (12) Magnifying glass or (and) colony counter.

[0041] (13) Staphylococcus aureus PetrifilmTM test piece.

[0042] (14) Staphylococcus aureus Petrifilm™ confirmation reaction sheet.

[0043] 2. Media and reagents

[0044] (1) 10% sodium chloride tryptone soybean broth.

[0045] (2) 7.5% sodium chloride broth.

[0046] (3) Blood agar plate.

[0047] (4) ...

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Abstract

The invention discloses a method for detecting staphylococcus aureus. The method includes the steps that 1, quantitative to-be-tested samples are weighed into quantitative premade culturing media to be subjected to enrichment culturing, and enrichment liquid is obtained; 2, quantitative enrichment liquid obtained in the step 1 is taken to be arranged in a testing slip to be tested, and the testing slip can display staphylococcus aureus colonies into claret-colored colonies; 3, the culturing result obtained in the step 2 is judged to detect the staphylococcus aureus. The invention further discloses a kit for detecting the staphylococcus aureus. When the staphylococcus aureus is detected through the method and / or the kit, operation is easy, convenient and rapid, and the detecting result is accurate and reliable.

Description

technical field [0001] The present invention relates to the field of microorganism detection, specifically, the present invention relates to the field of food microorganism detection, and more specifically, the present invention relates to a method for detecting Staphylococcus aureus and a kit for detecting Staphylococcus aureus. Background technique [0002] Staphylococcus aureus is an important pathogenic bacterium of zoonotic diseases. It is named for its spherical shape and arranged in clusters of grapes under the microscope. This bacterium is widely found in air, water, dust, humans and animals. in the excrement. Therefore, there are many opportunities for food and food raw materials to be contaminated by it. [0003] At present, the detection methods of Staphylococcus aureus at home and abroad can be mainly divided into two categories, one is the traditional microbial culture isolation and identification detection method, and the other is some methods based on molecul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/14C12Q1/06
Inventor 李雪晶马文丽薛志清喻东威宋晓东
Owner 蒙牛乳业(太原)有限公司
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