Epithelial tissue staining reagent and preparation method thereof

An epithelial tissue and dye technology, which is applied in the preparation of test samples, organic dyes, material inspection products, etc., can solve the problems such as the recognition rate and accuracy rate need to be improved, and achieves good reducibility, good solubility, and easy preparation. Effect

Active Publication Date: 2015-12-30
济宁博联生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0007] The above existing technologies all use methylene blue staining technology to identify malignant tumor cells, but the recognition rate and accuracy rate still need to be improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] An epithelial tissue staining agent, the composition includes the following components in terms of weight percentage: 5wt% of 5,6,7,8-tetrahydrofolate; 0.5wt% of methylene blue; 0.1wt% of horseradish peroxidase; Galactosamine 2wt%; N-phthaloyl chitosan 2wt%; medical grade glacial acetic acid 5wt%; deionized water balance.

[0021] Preparation method: 1) Dissolve 5,6,7,8-tetrahydrofolic acid in an aqueous solvent and let it stand for 24 hours; 2) Put methylene blue into the solution and stir for 2 hours; 3) Add horseradish peroxide to the solution Enzyme, galactosamine and N-phthaloyl chitosan; 4) Add glacial acetic acid solution until the pH of the solution is 5.

Embodiment 2

[0023] An epithelial tissue staining agent, the composition includes the following components in terms of weight percentage: cysteine ​​modified folic acid 10wt%; methylene blue 0.5wt%; horseradish peroxidase 1wt%; glucose 10wt%; N- Phthaloyl chitosan 5wt%; medical grade glacial acetic acid 2wt%; deionized water balance.

[0024] Preparation method: 1) Dissolve cysteine-modified folic acid in an aqueous solvent and let stand for 24 hours; 2) Put methylene blue into the solution and stir for 2 hours; 3) Add horseradish peroxidase, glucose and N - phthaloyl chitosan; 4) adding glacial acetic acid solution until the pH of the solution is 6.

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Abstract

The invention discloses an epithelial tissue staining reagent. The epithelial tissue staining reagent comprises components in percentage by weight as follows: 0.5%-15% of folic acid derivatives, 0.02%-10% of methylene blue, 0.1%-5% of horseradish peroxidase, 2%-10% of aminogalactose, 2%-10% of N-phthaloyl chitosan oligosaccharide, 2%-6% of medical glacial acetic acid and the balance of deionized water, wherein the folic acid derivatives preferably adopt one or more of 5,6,7,8-tetrahydrofolic acid, histidine N-acetyl-2-ethanediamide folic acid and cysteamine modified folic acid. The epithelial tissue staining reagent has the advantages as follows: the raw materials don't have irritation to skin and tract mucosa; the specificity is better; the selected folic acid derivatives are more easily combined with a folic acid receptor; saccharide derivatives have good reducibility and good solubility and are easy to prepare.

Description

technical field [0001] The invention relates to a dye composition for epithelial tissue, especially tumor tissue, and also relates to a preparation method of the dye. Background technique [0002] In recent years, the morbidity and mortality of malignant tumors are very high, and the mortality is closely related to early diagnosis and treatment. How to diagnose and treat early has become a hot research topic in the medical field in recent years. At present, the confirmation of malignant tumors is mainly based on pathological cytology examination and histological examination, which are diagnosed by changes in cell morphology and tissue structure. However, the cells of patients with early malignant tumors often only show an increase in nucleic acid that is difficult to diagnose, and there is no change in shape and structure, which is the main reason for the missed diagnosis of cytology and histology. At present, the staining and detection technology for tumor cells is develop...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09B67/42G01N1/30G01N33/52
Inventor 李家青王峰付建成
Owner 济宁博联生物科技有限公司
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