Preparation technology of tea catechins in fresh tea leaves
A technology of tea catechin and preparation process, applied in the direction of organic chemistry and the like, can solve the problems of high cost of materials, no prospect of industrialization amplification, environmental pollution, etc., and achieve the effect of broad application prospects.
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Embodiment 1
[0037] About 20g of fresh tea leaves were microwaved to inactivate the enzyme for 30 seconds, pulverized into fine particles by a powder machine, and 1g of pulverized fresh tea leaves were placed in a 150mL flat-bottomed Erlenmeyer flask, and the volume of water-saturated ethyl acetate added (A , mL), extraction temperature (B, ℃), extraction time (C, min), ethanol addition amount (D, mL) on the extraction efficiency of tea catechins. The experimental factor levels are shown in Table 1 below.
[0038] Table 1 Orthogonal experiment factor level value
[0039]
[0040] The arrangement sequence and absorbance value of the orthogonal experiment are recorded in Table 2 below. After the extraction, quickly pass the extract through a funnel lined with absorbent cotton, and dilute it in a 50mL volumetric flask with ethyl acetate. The ferrous tartrate method was used for quantitative color development of tea polyphenols-tea catechins in the extract.
[0041] Table 2 Orthogonal Ex...
Embodiment 2
[0048] Considering that ethyl acetate is an organic solvent with a low boiling point, combined with the above experimental results, under the premise that the mass volume ratio of fresh tea leaves (g) to water-saturated ethyl acetate (mL) is 1:10, further investigations were carried out at 25°C, 50°C, Effect of extraction time and extraction frequency on extraction efficiency of tea polyphenols-tea catechins. figure 2 A, B, C, and D represent the influence of extraction times on the absorbance value under the conditions of extraction at 25°C for 30 minutes, extraction at 50°C for 30 minutes, extraction at 25°C for 60 minutes, and extraction at 50°C for 60 minutes. Depend on figure 2 It can be seen that increasing the temperature within 30 min is beneficial to the dissolution of the target substance, but the color value of ferrous tartrate in the extract at 25 °C under the extraction time of 60 min is higher than 50 °C. It shows that after long-term high-temperature extracti...
Embodiment 3
[0050] Based on the above-mentioned examples, extract 1 mL of the fresh tea leaf extract with water-saturated ethyl acetate, add 0.5 mL to 1.5 mL of water, and 0.5 mL to 1 mL of petroleum ether. Color measurement of absorbance. The result is as Figure 4 As shown, with the increase of water volume, the concentration of tea polyphenols-tea catechins in the water phase decreased correspondingly, but 1mL of petroleum ether could promote more tea catechins into the water phase (compared to 0.5 mL of petroleum ether). That is to say, under the premise of fixing the volume ratio of extract solution and petroleum ether, the increase of water volume can make the target product transfer more into the water phase.
[0051] At the same time, the experiment also selected commercial tea polyphenols of 30% (w / w), 50% (w / w), and 90% (w / w) to investigate the effect of ethanol addition on the extraction effect of water-saturated ethyl acetate and water system. influences( Figure 5 ). In ...
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