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A kind of hybrid Liriodendron lhrgl1 gene and its application

A technology for hybridization of Liriodendron tulipifera and genes, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problems of unknown structure and function, slow progress, late start of research, etc. Effects of developmental delay

Active Publication Date: 2018-03-13
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the late start and slow progress of the research on woody plants, the number of genes encoding the DELLA protein in the hybrid tulip tree genome is still unclear, and its structure and function are unknown

Method used

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  • A kind of hybrid Liriodendron lhrgl1 gene and its application
  • A kind of hybrid Liriodendron lhrgl1 gene and its application
  • A kind of hybrid Liriodendron lhrgl1 gene and its application

Examples

Experimental program
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Embodiment 1

[0024] The material used in this example is the hybrid Liriodendron tulipifera leaves on the campus of Nanjing Forestry University, which were quickly frozen in liquid nitrogen after harvest and stored in an ultra-low temperature refrigerator (-80°C).

[0025] 1) Extraction of total RNA from hybrid tulip tree leaves

[0026] According to the instructions of the NORGEN Plant Total RNA Extraction Kit, the specific operation is as follows: add appropriate amount of materials and liquid nitrogen to the treated mortar, after fully grinding, add 600 μL LysisSolution; transfer the lysed liquid in the mortar to In a new centrifuge tube; after tightly closing the cap, invert and mix several times, let it stand at room temperature for 2 min to fully lyse, centrifuge at 12000 rpm for 2 min, and transfer the supernatant to a new centrifuge tube; add an equal volume 70% ethanol, vortex and mix; transfer the mixture to the column provided in the kit (connected to the 2mL collection tube), c...

Embodiment 2

[0050] Research indicates LhRGL1 and wxya It plays an important role in various tissues and organs of Liriodendron hybrida. However, the expression levels of the two genes were the highest in the stem, indicating that the GA signaling pathway was active during the growth and development of the stem, and DELLA protein played an important regulatory role in the elongation and growth of the stem.

[0051] The plant material used in this embodiment is Arabidopsis ( Arabidopsis thaliana )Col (Columbia) wild-type seeds, kindly donated by Professor Thomas Laux from Germany.

[0052] The Escherichia coli strain used in this embodiment is E. coli JM109; Agrobacterium strains GV3101 and EHA105 were used to transform Arabidopsis thaliana; the plant expression vector used in the experiment was pBI121. All strains were purchased from Related Biological Company.

[0053] 1) LhRGL1 Construction of gene pBI121 vector

[0054] With embodiment 1 obtaining LhRGL1 The full-length O...

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Abstract

The invention discloses a hybrid Liriodendron LhRGL1 gene and its application. The nucleotide sequence of the LhRGL1 gene is shown in SEQ ID NO.1, and the amino acid sequence of its expressed protein is shown in SEQ ID NO.2. In the present invention, through the cloning and identification of the hybrid Liriodendron LhRGL1 gene, gene expression analysis, and verification of its function, it is found that the growth and development of the LhPIN1 gene-transferred plant is retarded, the plant is dwarfed, and the number of branches increases. It can be seen that the gene will be used in plant breeding. There are a wide range of uses.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, and in particular relates to a hybrid Liriodendron LhRGL1 gene and an application thereof. Background technique [0002] Hybrid Liriodendron is a famous forest tree genetic breeder in my country, Professor Ye Peizhong, who uses the Chinese Liriodendron ( Liriodendron chinensis (Hemsl.) Sarg. ) and Liriodendron tulipifera ( Liriodendron tulipifera Linn. ) is an interspecific hybrid obtained through artificial hybridization, which has fast growth, beautiful tree posture, peculiar leaf shape, strong resistance, and fine texture texture. It is an important tree species for landscaping and industrial timber. Although hybrid Liriodendron has many advantages, hybrid seed production is limited by season and efficiency, which affects the popularization and application of hybrid Liriodendron. Due to the large number of plant somatic embryos, once the somatic embryos are formed, they can...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82C12N5/10A01H5/00A01H6/00
Inventor 陈金慧王鹏凯闫姗施季森董艺妮盛宇郑晨
Owner NANJING FORESTRY UNIV