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Method for rooting of soybean converted adventitious buds

A technology for rooting shoots and soybeans, applied in the field of transforming adventitious shoots and rooting of soybeans, can solve the problems of unfavorable physiological metabolism of regenerated seedlings, weak survival ability of rooted seedlings, and reduced survival rate of transplanting, so as to promote the occurrence of adventitious roots, improve the quality of rooting, and make The effect of cumbersome process

Inactive Publication Date: 2016-01-13
HEBEI NORMAL UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, it is necessary to prepare a solid rooting medium, which is complicated in technology and is easy to be infected with bacteria during the transfer of soybean adventitious buds to the medium and the subsequent cultivation process. The culture environment of the solid rooting medium is in a closed culture bottle. It is beneficial to the physiological metabolism of the growth of regenerated seedlings, inhibits the hairy roots of regenerated seedlings, and compared with the normal root system, the adventitious roots cultivated in the solid rooting medium have poor quality and no root hairs, and the absorption function is poor
The rooted seedlings cultivated in a closed environment in the bottle have weak survival ability, and usually have to go through the process of domestication outside the bottle, and then colonize. reduce

Method used

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Experimental program
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Embodiment 1

[0038] The method for transforming adventitious shoots and rooting of soybeans provided in the embodiment of the present invention specifically includes the following steps:

[0039] Soak the lower end of the soybean transformed adventitious buds in rooting liquid, then plant the soaked soybean transformed adventitious buds in an unclosed seedling bowl, cover the film, and carry out light cultivation. After the new leaves grow, remove the film and continue the cultivation Until the root system is developed, the regenerated seedlings are strong.

Embodiment 2

[0041] The method for transforming adventitious shoots and rooting of soybeans provided in the embodiment of the present invention specifically includes the following steps:

[0042] S21. Soak the lower end of the adventitious shoots of soybeans with a length greater than 3cm in the rooting solution for 5 minutes;

[0043] Among them, the composition of the rooting liquid is as follows: indole butyric acid 0.5 mg / L, naphthalene acetic acid 0.5 mg / L, cephalosporin 200 mg / L, pH=5.5;

[0044] S22. Put the seedling raising substrate into an unclosed seedling raising bowl, watering, and then implanting the soaked soybean transformed adventitious buds in step S21 into the unclosed seedling raising bowl containing the seedling raising substrate, and covering it with a film;

[0045] S23. The soybean transformed adventitious buds in step S22 are cultured under light, the time of light culture is 14 hours / day, the intensity of light culture is 3000LX, and the temperature of light culture is 22°...

Embodiment 3

[0048] The method for transforming adventitious shoots and rooting of soybeans provided in the embodiment of the present invention specifically includes the following steps:

[0049] S31. Soak the lower end of the soybean transformed adventitious shoot with a length greater than 3cm in the rooting liquid for 20 minutes;

[0050] The composition of the rooting liquid is as follows: indole butyric acid 1.0 mg / L, naphthalene acetic acid 0.2 mg / L, cephalosporin 300 mg / L, pH=6.0.

[0051] S32. Put the seedling raising substrate into an unclosed seedling raising bowl, watering, and then implant the soaked soybean transformed adventitious buds in step S31 into the unclosed seedling raising bowl containing the seedling raising substrate, and cover it with a film;

[0052] S33. The soybean transformed adventitious buds in step S32 are cultured under light, the time of light culture is 15 hours / day, the intensity of light culture is 6000LX, the temperature of light culture is 23℃, and the cover ...

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Abstract

The invention provides a method for the rooting of soybean converted adventitious buds. The rooting method comprises the following steps: soaking the lower ends of soybean converted adventitious buds in a rooting liquid, then planting the soybean converted adventitious buds in an opened seedling pot after soaking, covering a membrane, carrying out light culture, after new leaves grow out, removing the membrane, and keeping on culturing until seedling becomes strong. The used rooting liquid comprises simple components, and moreover, the operation is convenient. According to the provided method, the growth of agrobacterium is difficult before the adventitious buds generate roots or after the adventitious buds are transplanted to a rooting culture medium, and the rooting rate can reach 96.24%. According to the method, the rooting happens in an opened seedling pot (opened environment), the rooting quality can be improved, then the adventitious buds are cultured to obtain strong regeneration seedlings, thus the transplant survival rate of regeneration soybean seedlings is improved, the survival rate can reach 100%, and the success rate of soybean conversion is finally increased.

Description

Technical field [0001] The present invention relates to the field of biotechnology, in particular, to a method for soybean transformation and adventitious shoot rooting. Background technique [0002] Soybeans are commonly called soybeans, and legume soybeans are annual herbs. It is native to China and is cultivated all over China, and it is also widely cultivated all over the world. Soybean is one of China's important food crops. Northeast China is the main producing area. It is a crop whose seeds are rich in plant protein. Soybeans are most commonly used to make various soy products, extract soybean oil, brew soy sauce and extract protein. Okara or ground soybeans are also commonly used in livestock feed. [0003] In the 1980s, Monsanto researchers cloned the resistance gene (EPsPs gene) from the petunia, and introduced the EPsPs gene of the petunia into the soybean genome using Agrobacterium-mediated method, thereby cultivating glyphosate resistance. Phosphine soybean varietie...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 乔亚科董秋平乔潇李桂兰张锴
Owner HEBEI NORMAL UNIVERSITY OF SCIENCE AND TECHNOLOGY