Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of bionic porous tissue engineering cartilage scaffold preparation method

A porous tissue and engineering technology, applied in the field of biomedicine, can solve problems such as compound ratio transformation, and achieve the effect of improving cartilage quality

Active Publication Date: 2018-06-29
TAISHAN MEDICAL UNIV
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, Wu Hong and others have used human umbilical cord Wharton's jelly to construct tissue engineering scaffolds, which changed the physical structure of human umbilical cord Wharton's jelly, but did not carry out compound ratio modification according to the characteristics of cartilage extracellular matrix components

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of bionic porous tissue engineering cartilage scaffold preparation method
  • A kind of bionic porous tissue engineering cartilage scaffold preparation method

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0024] Such as figure 1 As shown, a kind of biomimetic porous tissue engineering cartilage scaffold preparation method of the embodiment of the present invention comprises the following steps:

[0025] S101: separate and prepare human umbilical cord Wharton's jelly, measure it, store it at low temperature, and crush it into a suspension;

[0026] S102: Prepare decellularized Wharton's gum suspension through repeated hypotonic freezing-thawing and enzymatic digestion;

[0027] S103: Mix the decellularized Wharton gum suspension with chondroitin sulfate in different proportions, add EDAC again and mix thoroughly, place at room temperature at a distance of 5-10 cm from the light source, and cross-link under the irradiation of 258nm wavelength ultraviolet light;

[0028] S104: Place the above cross-linked scaffolds in a -20°C refrigerator and a -80°C low-temperature refrigerator successively to shape the scaffolds, vacuum freeze-dry them in a vacuum freeze dryer to obtain shaped...

Embodiment 1

[0031] Step 1, separate and prepare human umbilical cord Wharton gum, measure it, store it at low temperature, and crush it into a suspension;

[0032] Step 2, preparing decellularized Wharton gum suspension through repeated hypotonic freeze-thaw and enzyme digestion;

[0033] Step 3: Mix the decellularized Wharton’s gum suspension with chondroitin sulfate, Wharton’s gum suspension: chondroitin sulfate = 2:0.25, add EDAC again and mix well, place at room temperature 5-10cm away from the light source Cross-linking under 258nm wavelength ultraviolet light irradiation;

[0034] Step 4: Place the above cross-linked scaffolds in a -20°C refrigerator and a -80°C low-temperature refrigerator to shape the scaffolds, freeze and dry them in a vacuum freeze dryer to obtain shaped scaffolds, store them in sealed bags, and irradiate at 60°C Sterilize and store at 4°C for later use.

Embodiment 2

[0036] Step 1, separate and prepare human umbilical cord Wharton gum, measure it, store it at low temperature, and crush it into a suspension;

[0037]Step 2, preparing decellularized Wharton gum suspension through repeated hypotonic freeze-thaw and enzyme digestion;

[0038] Step 3, mix the decellularized Wharton’s gum suspension with different proportions of chondroitin sulfate, the mass ratio of Wharton’s gum suspension to chondroitin sulfate is 2.0:0.75; add EDAC again and mix well, place at room temperature Cross-linking under the irradiation of 258nm wavelength ultraviolet light at a distance of 5-10cm from the light source;

[0039] Step 4: Place the above cross-linked scaffolds in a -20°C refrigerator and a -80°C low-temperature refrigerator successively to shape the scaffolds, vacuum freeze-dry them in a vacuum freeze dryer to obtain shaped scaffolds, store and seal them in sealed bags, and irradiate at 60°C Sterilize and store at 4°C for later use.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
densityaaaaaaaaaa
porosityaaaaaaaaaa
water absorptionaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for preparing a bionic porous tissue engineering cartilage scaffold. The decellularized Wharton gum suspension is mixed with chondroitin sulfate, and the mixing ratio of the Wharton gum suspension and chondroitin sulfate is 2.0:0.25-2.0:2.0 , add ethyl dimethylamine propyl carbodimethylamine again and mix thoroughly, place at room temperature 5-10 cm away from the light source for cross-linking under the irradiation of 258nm wavelength ultraviolet light; place the cross-linked scaffolds at low temperature successively The stent was shaped in a refrigerator, vacuum freeze-dried to obtain a shaped stent, stored in a sealed bag, sterilized by irradiation at 60°C, and stored at 4°C for use. The cartilage scaffold prepared by the present invention has the characteristics of bionic and multi-porosity, can induce stem cells to differentiate into chondrocytes in vitro, promote the expression of proteoglycan and type II collagen in the cartilage matrix, and can be used for constructing tissue-engineered articular cartilage in vitro, for repairing articular cartilage Defect provides high quality scaffold material.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a method for preparing a bionic porous tissue engineering cartilage support. Background technique [0002] Articular cartilage defects caused by various reasons such as trauma and infection are very common in clinic. Since articular cartilage has no blood supply, innervation and lymphatic circulation, its self-repair ability is poor. Once the area of ​​cartilage damage is large (>2.0cm), it is basically unable to repair itself, and it can even accelerate the degeneration of articular cartilage, resulting in joint dysfunction and affecting the quality of life of patients. Although the current treatment methods have many methods, such as microfracture, cartilage mosaic plastic surgery, subchondral bone drilling, etc., the long-term effect is not ideal, and it is difficult to restore hyaline articular cartilage. [0003] Since the concept of "tissue engineering" ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/20A61L27/50
Inventor 亓建洪宋洪强陈彬陈超谢地李文华周路
Owner TAISHAN MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com