Curdlan high-efficiency fermentation control method

Abstract

The invention discloses an efficient fermentation control method for gel polysaccharide. The method comprises the first step of first-level seed culture, the second step of second-level seed culture, the third step of fermentation and the fourth step of fermentation control, wherein in the procedure of the third step, according to zymophyte concentration detected online, a nutrient solution with the glucose content of 500 g / L is automatically supplied, the nutrient solution is automatically supplemented when the glucose content in the fermentation liquid is lower than 5 g / L, the zymophyte concentration is maintained between 100 g / L and 150 g / L, and in other words, the nutrient solution starts to be supplemented when the zymophyte concentration is lower than 100 g / L and stops being supplemented when the zymophyte concentration is higher than 150 g / L. According to the control method, the adding speed and adding amount of the nutrient solution is controlled in real time according to the numerical value of the zymophyte concentration, and the gel polysaccharide fermentation process is directly and accurately controlled in real time and becomes stable and efficient.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a high-efficiency fermentation control method of curdlan. Background technique [0002] Curdlan is a new microbial polysaccharide with many unique functions. In December 1996, after long-term safety and food toxicology tests, the FDA in the United States proved that curdlan is safe for use in food, and approved curdlan as a direct additive for food; curdlan Therefore, it is called the third food polysaccharide approved by FDA after xanthan gum and gellan gum. In addition to being used in the food industry, some scientists have recently studied the use of curdlan instead of dextran as the filler in the column for the chemical industry, and achieved better separation results. In the cosmetic industry, curdlan is also very effective as a thickener, suspending agent, stabilizer, moisturizer and rheology modifier, so it can be used in various types of cosmetics. According to analysis,...

AI Technical Summary

Problems solved by technology

[0006] To sum up, there is currently a lack of high-efficiency fermentation control methods for curdlan in this field. Because the generation of metabolic by-products cannot be effectively controlled when using glucose for fermentation, the waste of glucose and acid production during the fermentation process reduces the yield. Therefore, it is urgent to develop a method. On-line control method of curdlan fermentation with stable, high yield and low energy consumption