Preparation method and application of molecularly-imprinted electrochemiluminescence sensing material
A technology of molecular imprinting and sensing materials, applied in chemiluminescence/bioluminescence, analysis by chemical reaction of materials, etc., can solve the problems of no research, complicated operation, low sensitivity, etc., and achieve low detection limit and high sensitivity. , good stability
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Embodiment 1
[0020] (1) Preparation of gold nanoparticles functionalized with 2-mercaptoethanesulfonic acid and p-aminothiophenol:
[0021] Mix 10mL of ethanol solution containing 197mg of chloroauric acid with 5mL of methanol solution containing 42mg of sodium mercaptoethanesulfonate and 8mg of p-aminothiophenol, add 2.5mL of glacial acetic acid and incubate in an ice bath for 1 hour; then add 7.5mL of 1mol / L sodium borohydride aqueous solution was prepared into a colloidal gold solution; the above colloidal gold solution was stirred for 1 h under ice bath conditions and then continued to stir for 14 h under normal temperature conditions to obtain 2-mercaptoethanesulfonic acid and p-aminothiophenol functionalized Modified gold nanoparticle dispersion; the functionalized modified gold nanoparticles are washed and centrifuged twice with methanol and ethanol respectively, and vacuum-dried to obtain 2-mercaptoethanesulfonic acid and p-aminothiophenol functionalized gold nanoparticles;
[002...
Embodiment 2
[0031] Determination of MCPA content in actual samples:
[0032] Using the working electrode modified by the sensing material of the present invention to analyze and measure MCPA in actual samples (tap water, lake water, river water, oats, wheat), MCPA was not detected, and the standard addition method was used to carry out the standard addition recovery experiment. The electrochemiluminescence and cyclic voltammetry simultaneous detection mode of the LK5100 electrochemiluminescence analysis system was selected for scanning measurement of the sample solution to be tested, the scanning voltage was 0-2.2V, and the photomultiplier tube voltage was 800V. Read the luminous intensity after electrochemiluminescence quenching, and substitute it into (F 0 -F) / F 0 =0.1006logC MCPA +1.1480, calculated C MCPA . Three parallel measurements were performed, and the calculated recovery rate was 89.2-106.6%, indicating that the electrochemiluminescence sensor constructed by the sensing mat...
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