Preparation method and application of molecularly-imprinted electrochemiluminescence sensing material

A technology of molecular imprinting and sensing materials, applied in chemiluminescence/bioluminescence, analysis by chemical reaction of materials, etc., can solve the problems of no research, complicated operation, low sensitivity, etc., and achieve low detection limit and high sensitivity. , good stability

Inactive Publication Date: 2016-01-20
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Problems solved by technology

At present, the commonly used detection method is chromatography, which is complicated to operate and has low sensitivity.
Molecularly imprinted sensing materials are currently being used for the detection of multiple components, with simple operation and high accuracy, but there is no research on 2-methyl-4-chlorophenoxyacetic acid molecularly imprinted electrochemiluminescence sensing materials.

Method used

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  • Preparation method and application of molecularly-imprinted electrochemiluminescence sensing material

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Experimental program
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Effect test

Embodiment 1

[0020] (1) Preparation of gold nanoparticles functionalized with 2-mercaptoethanesulfonic acid and p-aminothiophenol:

[0021] Mix 10mL of ethanol solution containing 197mg of chloroauric acid with 5mL of methanol solution containing 42mg of sodium mercaptoethanesulfonate and 8mg of p-aminothiophenol, add 2.5mL of glacial acetic acid and incubate in an ice bath for 1 hour; then add 7.5mL of 1mol / L sodium borohydride aqueous solution was prepared into a colloidal gold solution; the above colloidal gold solution was stirred for 1 h under ice bath conditions and then continued to stir for 14 h under normal temperature conditions to obtain 2-mercaptoethanesulfonic acid and p-aminothiophenol functionalized Modified gold nanoparticle dispersion; the functionalized modified gold nanoparticles are washed and centrifuged twice with methanol and ethanol respectively, and vacuum-dried to obtain 2-mercaptoethanesulfonic acid and p-aminothiophenol functionalized gold nanoparticles;

[002...

Embodiment 2

[0031] Determination of MCPA content in actual samples:

[0032] Using the working electrode modified by the sensing material of the present invention to analyze and measure MCPA in actual samples (tap water, lake water, river water, oats, wheat), MCPA was not detected, and the standard addition method was used to carry out the standard addition recovery experiment. The electrochemiluminescence and cyclic voltammetry simultaneous detection mode of the LK5100 electrochemiluminescence analysis system was selected for scanning measurement of the sample solution to be tested, the scanning voltage was 0-2.2V, and the photomultiplier tube voltage was 800V. Read the luminous intensity after electrochemiluminescence quenching, and substitute it into (F 0 -F) / F 0 =0.1006logC MCPA +1.1480, calculated C MCPA . Three parallel measurements were performed, and the calculated recovery rate was 89.2-106.6%, indicating that the electrochemiluminescence sensor constructed by the sensing mat...

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Abstract

The invention discloses a preparation method and application of a molecularly-imprinted electrochemiluminescence sensing material based on quantum dot electrochemiluminescence for adsorbing trace 2-methyl--chlorophenoxy acetic acid (MCPA). The preparation method of the sensing material comprises the steps that gold nanoparticles and a CdSe-ZnS quantum dot function material which are functionally modified with sodium 2-mercaptoethanesulfonate and p-hydroxy thiophenol are prepared, the MCPA and the prepared function material is mixed, the surface of a gold electrode is modified with the material through electrochemical polymerization film forming, and an electrode modified with diphenylamine-crosslinked nanogold and a quantum dot network array molecularly-imprinted electrochemiluminescence material is prepared after the MCPA is eluted. The preparation method has the advantages that the quantum dot molecularly-imprinted electrochemiluminescence sensing material is synthesized through one step by adopting an electropolymerization method; the sensing material combines the electrochemiluminescence of quantum dots and the specific recognition of molecularly-imprinted polymers, the defects existing in an existing method for preparing the solid luminescence electrode are overcome, and the wide application prospect is achieved.

Description

technical field [0001] The invention belongs to the technical field of preparation of photoelectrochemical sensing materials, and in particular relates to a preparation method of an adsorbed trace amount of 2-methyl-4-chlorophenoxyacetic acid molecularly imprinted electrochemiluminescence sensing material based on quantum dot electrochemiluminescence and application. Background technique [0002] In recent years, with the continuous development of nanotechnology, quantum dots have developed rapidly as a new type of luminescent material. Their excellent luminescent properties have attracted the attention of many scientific researchers. They are widely used in sensing detection, biological imaging, biological probes and other fields. . It has been reported in the literature that quantum dot modified electrodes can be used for the detection of electrochemiluminescence. However, these methods lack specificity and cannot selectively adsorb the target and achieve accurate detect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76
Inventor 方国臻杨钰昆王俊平王硕
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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