Application of dimethyldiguanide with flavonoids in treating breast cancer and products of dimethyldiguanide
A metformin and breast cancer technology, applied in the field of medicine, can solve the problem of drug combination without teaching effect
Inactive Publication Date: 2016-01-27
SHANDONG NORMAL UNIV
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Problems solved by technology
The drug gdc0941 is a PI3K inhibitor. One of the main anti-cancer mechanisms of metformin is to inhibit mTOR by activating AMPK. This invention combines the two drugs based on the same mechanism of action of metformin hydrochloride and gdc0941, but has no effect on the mechanism of action. Combinations of different drugs have no teaching effect
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[0028] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.
[0029] Materials and Instruments:
[0030] name
source
Sigma, USA
flavonoids
Sigma, USA
DMEM medium
Beijing Maichen Technology, China
Hangzhou Sijiqing, China
Beijing Maichen Technology, China
MTT
Solaibo Biotechnology, China
DMSO
Solaibo Biotechnology, China
96-well plates
Costar, United States
Apoptosis and Necrosis Detection Kit
Biyuntian Biotechnology Co., Ltd., China
PI3k antibody
Akt antibody
p53 antibody
bioworld
Bax antibody
bioworld
[0031] Bcl-2 antibody
Wuhan Mitaka
Actin antibody
Zhongshan Golden Bridge
Western and PI Lysis Buffer, PMSF
Biyuntian Biotechnology Co., L...
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The invention discloses an application of dimethyldiguanide with flavonoids in treating breast cancer and products of dimethyldiguanide and provides a pharmaceutical combination mode using dimethyldiguanide as an anticancer drug with a natural product flavonoids serving as an aid. By adding the aid flavonoids, the anticancer functional density or time of the dimethyldiguanide is decreased, and a new way to develop the dimethyldiguanide as anticancer drugs is provided. The mode is: during a process where breast cancer cells cultured in vitro are treated using the dimethyldiguanide having a concentration of 20 nM, flavonoids having a concentration of 1000 Mum, and the ability of the dimethyldiguanide to inhibit the proliferation and induced death of breast cancer cells is thus improved by controlling PI3K / Akt / p53 signal channels and Bcl-2 family proteins.
Description
technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to an application of metformin combined with flavonoids in treating breast cancer and a product thereof. Background technique [0002] Breast cancer is one of the most common cancers in women, and the incidence rate of breast cancer accounts for the first place among female malignant tumors. Since the 1990s, the growth rate of the incidence of breast cancer in China is more than twice the global average rate. Every year, newly diagnosed cases and deaths of breast cancer in China account for 12.2% and 9.6% of the world's total respectively (Fan et al., 2014). [0003] Metformin is a biguanide drug containing two methyl groups, and it is one of the widely used antihyperglycemic drugs, which is safe and cheap. More and more reports point out that metformin has the effect of treating breast cancer. The anti-cancer mechanism of metformin mainly involves the following aspe...
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Login to View More IPC IPC(8): A61K36/00A61K31/352A61K31/155A61P35/00A61P15/14
Inventor 李国荣郑兆娣朱文振
Owner SHANDONG NORMAL UNIV