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Preparation method for multistage separation extraction of earthworm active proteins and method

A technology for active protein and multi-stage separation, which is applied in the field of multi-stage separation and extraction of earthworm active protein preparation devices, can solve the problems of large liquid holding capacity at the outlet of a foam tower, difficult protein enrichment ratio, and difficulty in draining the foam layer, and achieves improved performance. The effect of enrichment ratio and increasing recovery rate

Inactive Publication Date: 2016-01-27
TIANJIN BAIMING SCI & TECH DEV CO LTD
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AI Technical Summary

Problems solved by technology

[0004] The reason why it is difficult to increase the enrichment ratio of proteins in the foam separation aqueous solution is that the current research is only limited to the operation at room temperature
At room temperature, when the protein concentration in the solution exceeds its critical micelle concentration, it is difficult to drain the foam layer during the foam separation process, and the liquid holding capacity at the outlet of the foam tower is large, which makes it difficult to increase the protein enrichment ratio

Method used

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  • Preparation method for multistage separation extraction of earthworm active proteins and method

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preparation example Construction

[0020] A preparation method for multistage separation and extraction of earthworm active protein, comprising the following steps:

[0021] The collection detection of 1 earthworm protein homogenate feed liquid: collect earthworm protein homogenate feed liquid volume and be 2500ml, detect protein concentration and should be 30.79g / L and pH value 3.9;

[0022] II earthworm protein homogenate feed liquid pretreatment: use the pH value adjustment solution to adjust the pH value of the earthworm protein homogenate feed liquid described in the first step to 3.9, as the feed of the first stage foam separation process in step III;

[0023] III First-stage foam separation: Operate under the conditions of superficial gas velocity of 40-60ml / min and temperature of 50-60°C to break the foam layer until the foam no longer overflows from the top of the constant temperature foam separation column 1 Stop aeration, collect foam and carry out defoaming in foam collecting device 5, defoaming liq...

specific Embodiment 1

[0029] Preparation materials used in this specific example: Daping No. 2 earthworm, Baiming Technology Co., Ltd.; Coomassie Brilliant Blue G250 biological reagent.

[0030] The specific model of the preparation equipment used: rotameter 3WB, Changzhou Shuanghuan; foaming tower with sieve plate 610mm×80mm, customized by Beijing Xinwell; air pump FB-280, Shanghai Jaguar; spectrophotometer L6S, Shanghai Jingke; constant temperature water bath HH-3, Jiangsu Ronghua.

[0031] making process:

[0032] Wash 100g of fresh earthworms with water, add 250ml of distilled water to make a coarse homogenate, centrifuge at 4000r / min for 20min, and collect the supernatant; add 250ml of distilled water to the sediment to continue homogenization, centrifuge at 4000r / min for 20min, collect the supernatant; repeat extraction Until the supernatant is clarified (10 times in total). Combine the supernatants from the previous several times, adjust the pH value to 3.9, and raise the temperature to 55...

specific Embodiment 2

[0036] Preparation materials used in this specific example: Daping No. 2 earthworm, Baiming Technology Co., Ltd.; Coomassie Brilliant Blue G250 biological reagent.

[0037]The specific model of the preparation equipment used: rotameter 3WB, Changzhou Shuanghuan; foaming tower with sieve plate 610mm×80mm, customized by Beijing Xinwell; air pump FB-280, Shanghai Jaguar; spectrophotometer L6S, Shanghai Jingke; constant temperature water bath HH-3, Jiangsu Ronghua.

[0038] making process:

[0039] Wash 100g of fresh earthworms with water, add 250ml of distilled water to make a coarse homogenate, centrifuge at 4000r / min for 20min, and collect the supernatant; add 250ml of distilled water to the sediment to continue homogenization, centrifuge at 4000r / min for 20min, collect the supernatant; repeat extraction Until the supernatant is clarified (10 times in total). Combine the previous supernatants, adjust the pH value to 3.9, and raise the temperature to 50°C with a water bath. T...

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Abstract

The invention discloses a preparation method for multistage separation extraction of earthworm active proteins and a method. The preparation device comprises a constant temperature foam separating column, an air pump, an air distributer, a rotor flowmeter and a foam collection device. The preparation method comprises the following steps: firstly, collection and detection of an earthworm protein homogenate material liquid is carried out; secondly, the earthworm protein homogenate material liquid is subjected to pretreatment; thirdly, first stage foam separation is carried out; fourthly, second-stage foam separation is carried out. A two-stage foam separation technology is established at a high temperature based on influences on earthworm active protein foam separating effects, and the two-stage foam separation technology can raise the enrichment ratio and can raise the recovery rate.

Description

technical field [0001] The invention relates to the field of earthworm protein separation and extraction, in particular to a multi-stage separation and extraction earthworm active protein preparation device and method thereof. Background technique [0002] There are many kinds of active proteins with economic value in earthworms. At present, active protein extraction methods mainly include biochemical method, salting out method, membrane separation method and ion exchange resin method. However, these methods have defects such as high investment and production costs, complex operation process, etc., which increase the cost of the enterprise and reduce the profit of the enterprise. Therefore, it is necessary to explore a new process with simple operation, low cost and no pollution. [0003] Foam separation is an important separation technology that has developed rapidly in recent years. It is based on the principle of surface adsorption, through bubbling, the solute or part...

Claims

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Application Information

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IPC IPC(8): C07K1/14
Inventor 任建吴兆亮童应凯王英超孙玉涛
Owner TIANJIN BAIMING SCI & TECH DEV CO LTD
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