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A high-efficiency degradation bacterium ii83 of lignocellulosic substances and its application
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A lignocellulose-based, high-efficiency degrading bacteria technology, applied in the direction of bacteria, microorganisms, and methods based on microorganisms, can solve the problems of low enzyme activity and achieve stable enzyme activity, stable enzyme production, and rapid value-added effects
Active Publication Date: 2018-10-16
黑龙江省农业科学院牡丹江分院
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However, the use of fungi to degrade lignocellulose generally has the problem of low enzyme activity
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specific Embodiment approach 1
[0024] Specific embodiment one: a lignocellulosic material efficient degrading bacterium II83 of the present embodiment, it is Klebsiella pneumoniae (Klebsiella pneumoniae) II83, is preserved in the common microorganism center of China Committee for Microorganism Culture Collection, preservation address It is No. 3, No. 1 Courtyard, Beichen West Road, Chaoyang District, Beijing. The preservation date is December 12, 2014, and the preservation number is CGMCC No.10168.
[0025] The Klebsiella pneumoniae (Klebsiella pneumoniae) II83 of the present embodiment is a Gram-negative bacterium, and the shape of the bacterial strain is rod-shaped, and the size of the bacterial cell is 0.62-0.71×1.5-1.6 μm, does not form spores, and has no flagella , with capsules,; formed round, opaque, milky white, smooth protrusions, and neatly bordered colonies on LB medium (such as figure 1 shown).
[0026] The results of the physiological and biochemical reactions of the strain included 18 items a...
specific Embodiment approach 2
[0087] Embodiment 2: The application of a lignocellulosic substance-degrading bacteria II83 in this embodiment is used for degrading lignocellulosic substances.
[0088] Bacterial strain of the present invention is carried out following functional detection:
[0089] The specific embodiment of Klebsiella pneumoniae (Klebsiella pneumoniae) II83 was carried out to measure the weight loss rate and lignin, cellulose and hemicellulose degradation of bagged fungus waste and corn stalks to verify its unique functions. details as follows:
[0090] 1. Determination of weight loss rate of bagged fungus waste and corn stalks
[0092] Corn stalks were taken from the experimental field of the laboratory in Hulan Campus, Heilongjiang University, Harbin City, Heilongjiang Province in October 2012, dried, crushed and passed through a 40-mesh sieve, and set aside.
[0094] Bags of fungus waste were provided by Mudanjiang Bran...
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Abstract
The invention relates to a lignocellulose substance efficient degradation bacterium II83 and an application thereof. The lignocellulose substance efficient degradation bacterium II83 is Klebsiella pneumoniae II83 and is collected in the China General Microbiological Culture Collection Center (CGMCC) on December 12, 2014, with collection number of CGMCC NO. 10168. The lignocellulose substance efficient degradation bacterium II83 is used for degrading lignocellulose substances. The lignocellulose substance efficient degradation bacterium II83 has the beneficial effects that the strain is rapid in proliferation, has strong adaptability and is widely applied; the weight loss ratios of waste agaric material substitutes subjected to fermentation treatment with Klebsiella pneumoniae II83 are more than 46%; and the weight loss ratios of corn straws subjected to fermentation treatment with Klebsiella pneumoniae II83 are more than 26%.
Description
technical field [0001] The invention relates to a highly efficient degrading bacterium II83 of lignocellulosic substances and its application. Background technique [0002] my country is a large agricultural country, and the amount of agricultural waste generated is extremely huge. Due to the limitations of economic benefits and technology popularization, most of the agricultural waste is extensively and inefficiently used and the state of idleness is serious, resulting in waste of resources and environmental pollution. Waste has become China's largest source of pollution. However, there is a large amount of lignocellulose in agricultural waste, which needs to be developed and utilized urgently. [0003] Lignocellulose is the second most abundant organic renewable resource after cellulose in nature, and it is also one of the most difficult components for microorganisms to degrade. In recent years, some lignin-degrading fungi have begun to be applied in practice, but further...
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