Preparation of colloidal gold immunochromatography detection card for detecting sodium cyclamate
A technology of immunochromatography and cyclamate, which is applied in the field of colloidal gold immunochromatography detection card for detecting cyclamate, to achieve the effect of fast detection, high sensitivity and convenient use
Inactive Publication Date: 2016-01-27
JIANGSU WISE SCI & TECH DEV
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In September 1982, Abbott Laboratories and Energy Control Committee, on the basis of a large number of experimental facts, proved the safety of cyclamate with the latest research facts. Many international organizations also published a large number of comments one after another clearly stating that cyclamate is a safe substance, but The FDA has yet to conclusively address this issue
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[0012] 1. Preparation of Labeled Colloidal Gold
[0013] Preparation of colloidal gold solution: 1 g of chloroauric acid (HAuCl 4 ) powder dissolved in 100mL ultrapure water to prepare 1% chloroauric acid solution; then take 1L ultrapure water and heat it on a special electric furnace, add 10mL 1% trisodium citrate aqueous solution after boiling, continue heating for 5 minutes Then add 10mL of 1% chloroauric acid solution, heat it to boiling again, wait for the color to turn into wine red, start timing, stop heating after 10 minutes, take it off, cool naturally, and set the volume to 1L, which is the colloidal gold solution;
[0014] Antibody labeling: 0.1mol / lK required for determination of antibody 2 CO 3 ratio, take 100mL colloidal gold solution, add this ratio of 0.1mol / L K 2 CO 3 , mix well (about 5min), then dissolve and dilute cyclamate and the second animal protein with PBS (0.01mol / L, pH7.4) to 2mg / mL, add 2mL2mg / mL cyclamate antibody (or 2mL2mg / mL the second an...
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Abstract
The invention discloses preparation of a colloidal gold immunochromatography detection card for detecting sodium cyclamate, and is mainly used for rapid detection of the content of sodium cyclamate in food. According to the method, a sample liquid absorbing part, a colloidal gold labeling part, a detection reaction part and a water absorbing part are orderly stuck on a backing of test paper. The detection reaction part is coated by an antigen which is applied to detection and is used as a detection line, and at the same time, is coated by one bar of an IgG which resists the second species of animal protein and is used as a reference line. The rapid detection test paper strip has high specificity, can carry out semi quantitative detection, can be used at the environmental temperature of 4 to 35 DEG C, and is suitable for an individual farmer, a food hygiene and quality inspection department, customs and other animal-derived food for rapid detection on sodium cyclamate residues. The colloidal gold immunochromatography detection card has the beneficial effects of high specificity, high sensitivity, simple and convenient operation and the like, and can carry out semi-quantitative detection.
Description
technical field [0001] The invention belongs to the field of food safety testing, and specifically relates to a detection method for harmful residues in food, especially a colloidal gold immunochromatographic detection card for detecting cyclamate. Background technique [0002] Sodium cyclamate (English name: (Beet)molasses; SodiumN-cyclohexylsulfamate; Sodiumcyclamate) chemical name is sodium cyclamate, molecular formula: C 6 h 11 NHSO 3 Na, white needle, flaky crystal or crystalline powder, odorless, sweet taste, the sweetness of its dilute solution is about 30 times that of sucrose. The sweetness is 40-50 times that of sucrose, and it is a non-nutritive sweetener. 10% aqueous solution is neutral (PH value 6.5), stable to heat, light and air. Slightly bitter after heating. The decomposition temperature is about 280°C, and no caramelization reaction occurs. Slightly decomposed in acidic environment, stable in alkaline. Soluble in water (1g / 5ml) and propylene glycol (...
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IPC IPC(8): G01N33/53G01N1/28G01N33/531
Inventor 洪霞吴明慧杜霞
Owner JIANGSU WISE SCI & TECH DEV