Preservation reagent for mesenchymal stem cells and application thereof

Abstract

The invention provides a preservation reagent for mesenchymal stem cells and application of the preservation reagent to preserving the mesenchymal stem cells. The preservation reagent is prepared from 80-100 mmol / L glucose, 40-50 mmol / L mannitol, 24-28 mmol / L dipotassium hydrogen phosphate, 12-16 mmol / L potassium dihydrogen phosphate, 3-4 mmol / L adenine, 1-1.5 mmol / L inosine, 14-18 mmol / L potassium citrate, 35-45 mmol / L ammonium chloride, 75-80 mmol / L sodium chloride and cellular antioxidants. Due to the adoption of the preservation reagent for the mesenchymal stem cells, basic life metabolism of the cells is maintained through glucose, potassium citrate and other few nutrients, and meanwhile no large number of rich nutrients are added so that fast metabolism and proliferation of the cells can be avoided; by means of further assistance of adenine, inosine and mannitol, the shapes and the characters of the cells are maintained, the purposes of protecting cell membranes and enzyme activity are achieved, and the cell activity does not degrade in the preservation process; finally, oxidization damage of the cells is inhibited through the cellular antioxidants, and compared with existing common preservation liquid, the cells have better activity and quality after being preserved.

Description

technical field [0001] The invention belongs to the technical field of cell preservation solutions, and in particular relates to a preservation reagent for bone marrow mesenchymal stem cells and an application thereof. Background technique [0002] Stem cell therapy is to culture stem cells with continuous self-reproduction ability and pluripotency potential in vitro, and then transplant them into damaged or defective tissues of patients, so as to realize the supplement and repair of damaged tissues. At present, there are very few cells that can achieve the isolation and large-scale culture of human bone marrow mesenchymal stem cells. After the completion of cell culture, it is also necessary to perform phenotype detection, cell shape analysis, etc. on the cells, and then store the cells in the preservation solution. , to be infused when needed. In the stage of preservation after human bone marrow mesenchymal stem cells are cultured, it is necessary to maintain the basic li...

AI Technical Summary

Problems solved by technology

[0003] However, in the process of cell preservation, if the above-mentioned preservation solution of MSCs is used for preservation, the properties and changes of the cells can be maintained at a relatively normal level when the required preservation time is short (within 4 hours), and the degree of reduction in cell activity will not be greatly reduced. reduce the quality of treatment

However, if the storage time is further increased, when the storage is carried out for a longer period of time, first of all, the cells will have phenotypic changes, decreased activity, aggregation and other tendencies and trait changes will be more obvious, which will reduce the quality of the cells and affect the effect of treatment; However, the above-mentioned preservation solution itself does not have the ability to alleviate or reduce these problems in the preservation process. After preservation, the cell activity is greatly reduced, and it gradually turns to dormancy or apoptosis.