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Industrial method for preparing anthocyanidin from sambucus williamsii fruits

An anthocyanin and elderberry technology, which is applied in chemical instruments and methods, organic chemistry, azo dyes, etc., can solve the problems that the product purity is not significantly improved and the impurities adsorbed are many.

Active Publication Date: 2016-02-03
DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the commonly used purification method is mainly carried out by using macroporous adsorption resin. The advantage of macroporous adsorption resin is that it has a large adsorption capacity, can be used repeatedly, and can save costs at a certain cost; the disadvantage is that there are more impurities adsorbed. The product purity improvement is not very significant

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] After mixing 10 kg of elderberry fruit with 50 kg of aqueous solution with a pH value of 2.5, extract it with a high-voltage pulse electric field extractor. The extraction temperature is 35°C, the extraction time is 60 minutes, the electric field strength is 13kv / cm, and the number of pulses is 20. Collect the extract; the extract is pretreated with a microfiltration membrane and then treated with an ultrafiltration membrane. The materials of the microfiltration membrane and the ultrafiltration membrane are all selected from vinylidene fluoride (PVDF), and the pore size of the microfiltration membrane is 0.1um. The operating pressure is 0.15MPa, the feed liquid temperature is 35°C, the pore size of the ultrafiltration membrane is 30000Dal, the feed liquid temperature is 35°C, the ultrafiltration pressure is 0.30MPa, and the ultrafiltration time is 60min, the filtrate is collected; the filtrate Treat with macroporous adsorption resin, choose D101 for macroporous adsorptio...

Embodiment 2

[0019] Mix 10 kg of elderberry fruit with 50 kg of aqueous solution with a pH value of 2.9 and then use a high-voltage pulse electric field extractor to extract. The extraction temperature is 30°C, the extraction time is 50 minutes, the electric field strength is 15kv / cm, and the pulse number is 17. Collect the extract; the extract is pretreated with a microfiltration membrane and then treated with an ultrafiltration membrane. The materials of the microfiltration membrane and the ultrafiltration membrane are all selected from vinylidene fluoride (PVDF), and the pore size of the microfiltration membrane is 0.2um. The operating pressure is 0.15MPa, the feed liquid temperature is 30°C, the pore size of the ultrafiltration membrane is 50000Dal, the feed liquid temperature is 30°C, the ultrafiltration pressure is 0.25MPa, and the ultrafiltration time is 50min, the filtrate is collected; the filtrate Treat with macroporous adsorption resin, choose D101 for macroporous adsorption resi...

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Abstract

The present invention belongs to the field of natural organic chemistry, and relates to an extraction and separation method for anthocyanidin derived from wild sambucus williamsii. According to the method, anthocyanidin is extracted by using high voltage pulse electric field extraction equipment, and then anthocyanidin purification is performed by using membrane equipment and a macroporous adsorption resin so as to obtain the high-purity anthocyanidin product. The industrial method of the present invention has the following advantages that: 1, with the high voltage pulse electric field technology, the extraction solvent can well enter the internal of the plant cells so as to achieve the efficient anthocyanidin extraction; and 2, with the application of the membrane separation technology and the macroporous adsorption resin purification technology to perform the anthocyanidin purification, the purification effect is significant compared with the single use of the macroporous adsorption resin. The prepared anthocyanidin of the present invention can be widely used in the fields of health food, cosmetics, and the like.

Description

technical field [0001] The invention belongs to the field of natural organic chemistry, and relates to a method for preparing anthocyanins from Daxinganling wild elderberry fruit. Background technique [0002] Anthocyanin is a water-soluble pigment that can change color with the acidity and alkalinity of the cell fluid. The cell fluid is acidic and reddish, and the cytosol is alkaline and blue. Anthocyanin is one of the main pigments that make up the color of petals and fruits. Anthocyanins are secondary metabolites of plants, which play an important role in physiology. Anthocyanins are pure natural anti-aging nutritional supplements. Studies have proved that they are the most effective plant antioxidants found by humans today. Its antioxidant properties Fifty times higher than vitamin E and twenty times higher than vitamin C. Its bioavailability to the human body is 100%, and it can be detected in the blood 20 minutes after taking it. It has a significant effect on enhanc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62C09B61/00C09B67/54
CPCC07D311/62C09B61/00
Inventor 姚德坤苏学辉
Owner DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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