A kind of preparation method and application of agricultural root-knot nematode composite biocontrol agent
A root-knot nematode and biocontrol fungicide technology, applied in the field of agricultural microorganisms, can solve problems such as crop quality decline, and achieve the effects of promoting crop growth, inhibiting plant pathogens, and having obvious market competitiveness.
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Embodiment 1
[0029] Example 1 Preparation of Agricultural Root Knot Nematode Composite Biocontrol Agent by Shallow Tray Culture
[0030] Pick 1 ring from the strains preserved on the slant of the test tube and inoculate it in the activation medium, and culture it at 30°C and 200 rpm for 36 hours until the bacteria are turbid to obtain the activated seed liquid; the activation medium (g / L): Peptone 10, yeast powder 5, glycerin 25, chitosan powder 2, potassium dihydrogen phosphate 3, magnesium sulfate 3, disodium hydrogen phosphate 3, pH 6.5.
[0031] Solid fermentation medium used: 30% dry brewer’s grains, 8% bran, 6% crushed peanut shells, 5% soybean peptone, 7.5% powdered chitosan (deacetylation degree above 90%), 2.25% crab shell powder %, NaCl 0.5%, CaCl 2 0.3%, MgSO 4 0.3%, FeSO 4 0.15%, according to the conversion of the total mass of the medium components, add purified water to reach a water content of 40%. Preparation method: first dissolve NaCl and CaCl with water 2 , MgSO ...
Embodiment 2
[0034] Embodiment 2 solid fermenter prepares agricultural root-knot nematode composite biocontrol agent
[0035] Pick 1 ring from the strains preserved on the slant of the test tube and inoculate it in the activation medium, and culture it at 28°C and 200 rpm for 48 hours until the bacteria are turbid to obtain the activated seed solution; the activation medium (g / L): Peptone 5, yeast powder 3, glycerin 20, powdered chitosan 1, potassium dihydrogen phosphate 2, magnesium sulfate 2, disodium hydrogen phosphate 2, pH 6.5.
[0036] The solid fermentation medium used: 17% dry brewer's grains, 4% bran, 3% crushed peanut shells, 2% soybean peptone, 2.55% powdered chitosan (deacetylation degree above 90%), and 1% crab shell powder %, NaCl 0.2%, CaCl 2 0.1%, MgSO 4 0.1%, FeSO 4 0.055%, according to the conversion of the total mass of the medium components, add purified water to reach a water content of 70%. Preparation method: first dissolve NaCl and CaCl with water 2 , MgSO ...
Embodiment 3
[0039] Example 3 Preparation of agricultural root-knot nematode composite biocontrol agent in ventilated fermentation tank
[0040] Pick 1 ring from the strains preserved on the slant of the test tube and inoculate it in the activation medium, and cultivate it at 28-30°C and 200 rpm for 36-48 h until the bacteria are turbid to obtain the activated seed liquid; the activation medium ( g / L): peptone 8, yeast powder 4, glycerin 22, powdered chitosan 2, potassium dihydrogen phosphate 2, magnesium sulfate 2, disodium hydrogen phosphate 3, pH 6.5.
[0041] Solid fermentation medium used: 30% dry brewer’s grains, 6% bran, 5% crushed peanut shells, 5% soybean peptone, 6% powdered chitosan (deacetylation degree above 90%), 2% crab shell powder %, NaCl 0.5%, CaCl 2 0.3%, MgSO 4 0.3%, FeSO 4 0.15%, according to the conversion of the total mass of the medium components, add tap water to a water content of 44.75%. Preparation method: first dissolve NaCl and CaCl with water 2 , MgSO ...
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