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A method for remediating harmful organic and/or heavy metal contaminated substrates

A technology of organic matter and heavy metals, which is applied in the field of environmental bioremediation, can solve the problems that ecological piles cannot remove heavy metals, high cost investment, slow effect of phytoremediation, etc., and achieve the effect of resource reuse and low cost

Active Publication Date: 2019-05-14
山东迈科珍生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there are many limitations to using biopiles and phytoremediation techniques
The construction and active maintenance of ecological piles require high energy consumption and high cost investment
In addition, the eco-pillar technology can only treat substrates contaminated with organic pollutants (such as petroleum hydrocarbons and polycyclic aromatic hydrocarbons)
In many cases, organic compound-contaminated soils often contain low concentrations of heavy metals, in which case biopiles cannot remove heavy metals and / or reduce their degradation efficiency due to the toxicity of heavy metals to microorganisms
The limitation of phytoremediation is that the effect of phytoremediation is very slow due to the fact that plants are not ideally suitable for the decomposition and metabolism of organic pollutants

Method used

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  • A method for remediating harmful organic and/or heavy metal contaminated substrates
  • A method for remediating harmful organic and/or heavy metal contaminated substrates
  • A method for remediating harmful organic and/or heavy metal contaminated substrates

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Embodiment 1: Restoration of the ecological pile system of TPH polluted soil

[0073] The soil of the food processing site is contaminated by mineral oil in the deep layer (2-4m below the surface layer), and the oil-contaminated soil needs to be excavated from the site at first. A total of 4870m was excavated 3 The analysis showed that the average level of TPH reached 1613ppm. Contaminated soil was stored in an unused parking lot (concrete floor). Then mix the nutrient salt (nitrogen to phosphorus ratio of 25:4) with the polluted soil, the ratio of fertilizer to soil is 5kg / m 3 . The soil was also added with TPH-degrading bacteria Pseudomonas putida (strain preservation number: ATCC: PCA5581) and TPH-degrading bacteria flora isolated from the site-contaminated soil. The degrading bacteria were isolated by adding 10g of TPH-contaminated soil to 500ml of deficient medium, cultured at 100rpm for 2 weeks, and then transferred to the same medium supplemented with diesel ...

Embodiment 2

[0088] Example 2: Remediation of polycyclic aromatic hydrocarbon-contaminated clay by ecological pile system

[0089] The PAH-contaminated soil came from a wood preservative processing plant. Analysis by a third-party laboratory shows that the level of polycyclic aromatic hydrocarbons in the pollution can reach 580ppm. The isolation method of PAH-degrading bacteria is as follows: add 10g of PAH-contaminated soil to 500ml of deficient medium, culture at 100rpm for 2 weeks, switch to a new deficient medium, and add a small amount of naphthalene, anthracene, phenanthrene, fluorene, and fluorescein every week. Anthracene as the sole carbon source. After cultivating for 5 weeks, the isolated polycyclic aromatic hydrocarbon degrading bacteria group and the known plant growth-promoting bacteria Pseudomonas (strain preservation number ATCC: 55089) and the bacterial strain Bacillus (bacterial strain) that can produce biosurfactants No. DSM: 3258) mixed and embedded into spherical col...

Embodiment 3

[0102] Embodiment 3: The ecological stack system remediates the soil polluted by oil sludge containing crude oil and heavy metals

[0103] Sludge-contaminated soil comes from oilfield companies. The soil was polluted by petroleum hydrocarbons and Cd with concentrations of 30 000ppm and 10ppm, respectively (measured by a third-party laboratory).

[0104] The isolation method of total petroleum hydrocarbon-degrading bacteria is as follows: add 10 g of petroleum hydrocarbon-contaminated soil into 500 ml defect culture medium (Abraham et al. Diesel oil was added to the culture medium weekly for 3 months, and then the inoculum was embedded with alginate. The number of microorganisms contained in each pellet is 10 8 -10 9 CFU. At the same time, polycyclic aromatic hydrocarbon degrading bacteria, plant growth-promoting bacteria and plant seeds are embedded in the colloid for plant coverage on the surface of the ecological pile.

[0105] The application ratio of embedded petroleu...

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Abstract

The present invention provides a method for the remediation of harmful organics and / or heavy metal contaminated matrix in order to remediate harmful organics and / or heavy metal contaminated soil, sediment and / or sludge. The method involves the processes of biostimulation, bioaugmentation, rhizoremediation, phytoremediation and a passive biopile. The method comprises steps of: a) designing a microbial agent comprising pollutant degrading bacteria, plant promoting bacteria and / or biosurfactant producing bacteria; b) preprocessing the matrix; c) constructing a passive biopile; and d) capping the biopile with plants for phytoremediation. The method achieves internationally-dominant green and persistent remediation while achieving bioremediation / phytoremediation degrading the organics and removing heavy metal.

Description

field of invention [0001] The invention relates to a method for remediating harmful organic matter and / or heavy metal-contaminated substrates, in particular to a method for passive biopile-plant joint remediation of soil or sediment polluted by organic pollutants and / or heavy metal mixtures, which belongs to environmental bioremediation technology field. Background technique [0002] Bioremediation can be defined as the degrading, consuming, breaking down, transforming, metabolizing and / or removing pollutants from the environment by using an ecosystem (including microorganisms, plants and enzymes), which can be in situ The repair can also be performed ectopically. Ecopile is an ex-situ bioremediation technology for soil and sediment, which has been widely used in the field of petrochemical-contaminated soil and sediment remediation. The construction of an ecological pile includes two parts: stacking contaminated soil / dry sediment into a pile, and stimulating the biodegrada...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B09C1/10
CPCB09C1/105
Inventor 刘雪梅柯瑞安·杰曼戴维·瑞恩戴维·道林
Owner 山东迈科珍生物科技有限公司
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