Method using 1,4-dihydroxy-9,10-anthraquinone thiosemicarbazone compound as fluorescent probe to detect copper ions

A technology of anthraquinone amino reduction and fluorescent probe, which is applied in the field of optical analysis and detection, and can solve problems such as increasing immune function

Inactive Publication Date: 2016-02-24
HENAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, anthraquinones also have the effects of hemostasis and blood circulation, promoting sleep, reducing blood lipids, and increasi

Method used

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  • Method using 1,4-dihydroxy-9,10-anthraquinone thiosemicarbazone compound as fluorescent probe to detect copper ions
  • Method using 1,4-dihydroxy-9,10-anthraquinone thiosemicarbazone compound as fluorescent probe to detect copper ions
  • Method using 1,4-dihydroxy-9,10-anthraquinone thiosemicarbazone compound as fluorescent probe to detect copper ions

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Experimental program
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Effect test

Embodiment 1

[0022] Optimization of experimental conditions

[0023] 1. Selection of Probe Molecular Concentration

[0024] Increasing the concentration of probe molecules can increase the absorbance and fluorescence intensity, which is beneficial to improve the selectivity of metal ions and the linear range of the standard curve. However, when the concentration is too high, the fluorescence intensity of the blank will also increase with the increase of the probe molecule concentration. If the concentration is too low, it is difficult to detect the fluorescence absorption peak after adding ion quenching. Considering the sensitivity and linear range of the method, The concentration of the probe was chosen to be 1.0 × 10 -5 mol / L.

[0025] 2. The choice of buffer solution

[0026] Add 1 mL of phosphate buffer solution to a 10 mL colorimetric tube, and then add 0.01 mL of a molar concentration of 1.0 × 10 -3 mol / L DMF-H of 1,4-dihydroxy-9,10-anthraquinone thiosemicarbazide 2 O solution, ...

Embodiment 2

[0033] Add 1 mL of phosphate buffer solution to multiple 10 mL colorimetric tubes, and then add 0.01 mL of 1.0 × 10 molar concentration. -3 mol / L DMF-H of 1,4-dihydroxy-9,10-anthraquinone thiosemicarbazide 2 O (DMF and H 2 The volume ratio of O is 1:99) solution, and then different concentrations of copper ion solutions ([Cu 2+ ](×10 -6 mol / L): 0.3, 0.5, 0.7, 1, 2, 3, 4, 5, 7, 9, 10), dilute to 10 mL with deionized water, shake and mix for 25 minutes, and measure the fluorescence intensity of each solution. The fluorescence intensity measurement conditions are: excitation slit 5nm, emission slit 10nm, excitation wavelength 488nm, emission wavelength 577nm, the solution changes from light red to blue-purple during the measurement process, image 3It is the fluorescence absorption spectrum of copper ion solutions of different concentrations. It can be seen from the figure that the red shift of the fluorescence absorption peak at 488nm appears a new fluorescence absorption pea...

Embodiment 3

[0037] Add 1 mL of phosphate buffer solution to multiple 10 mL colorimetric tubes, and then add 0.01 mL of 1.0 × 10 molar concentration. -3 mol / L DMF-H of 1,4-dihydroxy-9,10-anthraquinone thiosemicarbazide 2 O solution, and then respectively added the molar concentration of 1.0 × 10 -3 mol / L standard solutions of different metal ions (Na + , K + , Ca 2+ , Mg 2+ , Ni 3+ , Mn 2+ , Zn 2+ , Ba 2+ , Fe 3+ , Co 2+ , Cd 2+ , Pb 2+ , Ag 2+ , Hg 2+ different ions), dilute the volume to 10 mL with deionized water, and measure the fluorescence intensity of each solution after shaking (such as Figure 5 ), the results show that when the same concentrations of various metal ions interact with the probe molecule ESN, only Cu 2+ The addition of ions reduced the fluorescence intensity of the mixed system at 577 nm, resulting in fluorescence quenching. When other metal ions interacted with the probe molecules, the fluorescence intensity did not change significantly compared with...

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Abstract

The present invention discloses a method using 1,4-dihydroxy-9,10-anthraquinone thiosemicarbazone compound as a fluorescent probe to detect copper ions, and belongs to the technical field of optical analysis detection. The recognition effect of the 1,4-dihydroxy-9,10-anthraquinone thiosemicarbazone compound and various metal ions is investigated and studied experimentally, a good copper ion selective recognition effect of probe molecule ESN is found, due to the solubility of hydroxyl and water, the probe molecule can achieve the fluorescence identification of the copper ions in an aqueous solution, a better result can be achieved under physiological pH condition, the method is green, specific in copper ion selectivity and relatively high in sensitivity, and can play fluorescence quenching role in short reaction time, and the probe molecule can be successfully applied to the detection of the copper ions in biological living cells.

Description

technical field [0001] The invention belongs to the technical field of optical analysis and detection, and in particular relates to a method for detecting copper ions by using a 1,4-dihydroxy-9,10-anthraquinone thiosemicarbazide compound as a fluorescent probe. Background technique [0002] Some life activities in the living body are the research objects that people pay great attention to in the fields of life science, medicine and chemistry, especially the metabolic process of some metal trace elements in the life process. Trace metal elements are more or less related to some human diseases. In the past, due to the lack of certain technical means, it was difficult for people to study these issues in depth. However, in recent years, with the emergence of laser confocal microscopy scanning technology, it has provided more favorable means for the research of ion-fluorescence chemical sensors, and also provided a better way for the research of ion fluorescence chemical sensors. ...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/643G01N2021/6432
Inventor 时蕾潘峰刘统信麻娜娜张贵生刘青峰
Owner HENAN NORMAL UNIV
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